CAJ | 학술논문

目的调查山西省部分地区的虫媒病毒,在当地采集蚊虫标本进行病毒的分离与鉴定.方法 2007年7月和8月在当地采集蚊虫标本,通过组织细胞培养进行病毒分离,利用分子生物学和生物信息学技术对病毒分离物进行鉴定与分析.结果分离到10株病毒分离物,鉴定结果显示分离株SX0765、SX0766、SX0767、SX0771、SX0789、SX0790、SX0793、SX0794、SX0795、SX0796均为版纳病毒;此外,分离株SX0771和SX0794还同时存在辽宁病毒基因.进一步分析显示版纳病毒新分离株与此前在北京、云南和辽宁分离到的病毒株之间存在明显差异,同源性在89.7%～94.1%之间.结论在山西省分离到10株版纳病毒和两株辽宁病毒,版纳病毒与我国其他地区分离株有明显差异,在进化上相对独立.
목적 조사산서성부분지구적충매병독,재당지채집문충표본진행병독적분리여감정.방법 2007년7월화8월재당지채집문충표본,통과조직세포배양진행병독분리,이용분자생물학화생물신식학기술대병독분리물진행감정여분석.결과 분리도10주병독분리물,감정결과현시분리주SX0765、SX0766、SX0767、SX0771、SX0789、SX0790、SX0793、SX0794、SX0795、SX0796균위판납병독;차외,분리주SX0771화SX0794환동시존재료녕병독기인.진일보분석현시판납병독신분리주여차전재북경、운남화료녕분리도적병독주지간존재명현차이,동원성재89.7%～94.1%지간.결론 재산서성분리도10주판납병독화량주료녕병독,판납병독여아국기타지구분리주유명현차이,재진화상상대독립.
Objective To investigate arbeviruses in some regions of Shanxi province, isolation and identification for arbovirus activity from mosquitoes was conducted. Methods Mosquitoes were collected from these area in 2007 and then used for virus isolation by cell culture. The virus isolates were identified by molecular biology and the sequences were analyzed by bioinformaties. Results Ten Banns virus (strains SX0765, SX0766, SX0767 ,SX0771, SX0789, SX0790, SX0793, SX0794, SX0795, SX0796) were iselated, and two Liaoning virus were also isolated from isolates SX0771, SX0794. Phylogenetic tree of the Banns virus isolates showed that ten strains are located in a distinct branch from all of the other Chinese Banns virus isolates. The homology is between 89.7 % and 94.1%. Conclusion Ten Banna virus and two Lianning virus were isolated during this arbovirus investigation in Shanxi province. New Banns virus isolates showed a distinct phylogenetic relationship with the other Chinese Banns virus strains.