中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2008年
8期
544-549
,共6页
肖俊%朱玲燕%陈崴%聂静%陈文芳%董秀清%彭文兴%黄锋先%余学清
肖俊%硃玲燕%陳崴%聶靜%陳文芳%董秀清%彭文興%黃鋒先%餘學清
초준%주령연%진외%섭정%진문방%동수청%팽문흥%황봉선%여학청
肾小球肾炎,ISA%T淋巴细胞%CD4+CD25+调节性T细胞%Th1细胞%Th2细胞%叉头框蛋白3
腎小毬腎炎,ISA%T淋巴細胞%CD4+CD25+調節性T細胞%Th1細胞%Th2細胞%扠頭框蛋白3
신소구신염,ISA%T림파세포%CD4+CD25+조절성T세포%Th1세포%Th2세포%차두광단백3
Glomemlonephritis,IgA%T lymphocytes%CD4+CD25high regulatory Tcells%Th1 cells%Th2 cells%Forkhead box protein 3
目的 探讨CD4+CD25high调节性T细胞(Treg)及辅助性T细胞亚群(Th1、Th2)比例失衡在IgA肾病(IgAN)免疫发病机制中的作用.方法 用流式细胞仪检测IgAN患者外周血Treg及Th1、Th2的比例.以胞内染色技术检测叉头框蛋白3(FOXP3)的表达.Treg及Th1、Th2的比例与IgAN各项临床病理指标的相关性分析采用Spearman或Pearson相关分析法.结果 IgAN患者外周血中Treg比例明显高于健康人p(2.14±0.82)%比(1.59±0.53)%,P＜0.05],与血IgA水平呈正相关(r=0.397,P＜0.05),与eGFR呈负相关(r=-0.376,P＜0.05).IgAN患者外周血中Th2细胞比例显著高于健康对照组[(2.57±0.72)%比(1.81±1.10)%,P＜0.05],与血IgA水平呈正相关(r=0.468,P＜0.05).IgAN患者Th1/Th2比值显著低于健康对照组(5.75±1.89比12.73±9.79,P＜0.05),但与临床各指标间没有相关性.结论 IgAN患者体内存在T细胞亚群表达紊乱.Treg在外周血中的增多以及以,Th2为优势的Th1/Th2失衡可能在IgAN的发病中起重要作用.
目的 探討CD4+CD25high調節性T細胞(Treg)及輔助性T細胞亞群(Th1、Th2)比例失衡在IgA腎病(IgAN)免疫髮病機製中的作用.方法 用流式細胞儀檢測IgAN患者外週血Treg及Th1、Th2的比例.以胞內染色技術檢測扠頭框蛋白3(FOXP3)的錶達.Treg及Th1、Th2的比例與IgAN各項臨床病理指標的相關性分析採用Spearman或Pearson相關分析法.結果 IgAN患者外週血中Treg比例明顯高于健康人p(2.14±0.82)%比(1.59±0.53)%,P＜0.05],與血IgA水平呈正相關(r=0.397,P＜0.05),與eGFR呈負相關(r=-0.376,P＜0.05).IgAN患者外週血中Th2細胞比例顯著高于健康對照組[(2.57±0.72)%比(1.81±1.10)%,P＜0.05],與血IgA水平呈正相關(r=0.468,P＜0.05).IgAN患者Th1/Th2比值顯著低于健康對照組(5.75±1.89比12.73±9.79,P＜0.05),但與臨床各指標間沒有相關性.結論 IgAN患者體內存在T細胞亞群錶達紊亂.Treg在外週血中的增多以及以,Th2為優勢的Th1/Th2失衡可能在IgAN的髮病中起重要作用.
목적 탐토CD4+CD25high조절성T세포(Treg)급보조성T세포아군(Th1、Th2)비례실형재IgA신병(IgAN)면역발병궤제중적작용.방법 용류식세포의검측IgAN환자외주혈Treg급Th1、Th2적비례.이포내염색기술검측차두광단백3(FOXP3)적표체.Treg급Th1、Th2적비례여IgAN각항림상병리지표적상관성분석채용Spearman혹Pearson상관분석법.결과 IgAN환자외주혈중Treg비례명현고우건강인p(2.14±0.82)%비(1.59±0.53)%,P＜0.05],여혈IgA수평정정상관(r=0.397,P＜0.05),여eGFR정부상관(r=-0.376,P＜0.05).IgAN환자외주혈중Th2세포비례현저고우건강대조조[(2.57±0.72)%비(1.81±1.10)%,P＜0.05],여혈IgA수평정정상관(r=0.468,P＜0.05).IgAN환자Th1/Th2비치현저저우건강대조조(5.75±1.89비12.73±9.79,P＜0.05),단여림상각지표간몰유상관성.결론 IgAN환자체내존재T세포아군표체문란.Treg재외주혈중적증다이급이,Th2위우세적Th1/Th2실형가능재IgAN적발병중기중요작용.
Objective To investigate the effects of CD4+CD25high regulatory T cells(Treg)and the imbalance of helper T lymphocyte subsets(Th1/Th2)on the immunological mechanism of IgA nephropathy(IgAN)patients. Methods The percentage of Treg and helper T cells subpopulation (Th1/Th2)in the peripheral blood of IgAN patients and healthy controls was examined by flow cytometry.The FOXP3 expression was detected through intracellular staining.The correlation of Treg or Th1/Th2 with clinical parameters of IgAN was analyzed by Spearman or Pearson rank correlation test. Results The percentages of Treg and Th2 cells were significantly higher in peripheral blood of IgAN patients compared to that of healthy controls[Treg (2.14±0.82)%vs[1.59±0.53)%,Th2(2.57±0.72)%vs(1.81±1.10)%,all P＜0.05].Th1/Th2 ratio was significantly reduced in IgAN patients(5.75±1.89 vs 12.73±9.79,P＜0.05).The percentage of circulating Treg cells was positively correlated with serunl IgA concentration(r=0.397,P＜0.05),and was negatively correlated with eGFR(r=-0.376,P＜0.05).The percentage of circulating Th2 cells was positively correlated with serum IgA(r=0.468,P＜0.05). Conclusions There is a disorder of T lymphocyte population in the peripheral blood of IgAN patients.The increased Treg and Th2 cells may play an important role in the pathogenesis of IgAN.
