CAJ | 학술논문

目的观察纳米金(GNP)对人微血管周细胞增殖和G蛋白信号调节蛋白5(RGSS)表达的影响.方法制备浓度为50 nmol/L直径分别为25、50、100 nm的GNP溶液；体外培养人微血管周细胞株HBVP-1200；光学显微镜观察GNP作用周细胞、在细胞中沉积；噻唑蓝(MTT)比色法检测周细胞增殖；原子力显微镜( AFM)观察周细胞表面形貌；Western blot检测周细胞RGS5蛋白表达.结果 (1)不同组间的GNP( 25、50、100 nm)及对照组,其周细胞增殖率分别为:(147.9±5.9)％、(121.7±3.4)％、(107.6±2.1)％及( 100.0±0.0)％；粒径越小,周细胞增殖率越高(P＜0.05).(2)AFM观察到粒径越小的GNP处理后,周细胞表而形貌变化越明显.主要表现在细胞膜内陷、细胞表面出现较大孔洞,有细胞内吞现象. (3)Western blot检测到粒径越小的GNP,抑制RGS5蛋白表达越明显.结论 GNP促进人微血管周细胞增殖,抑制周细胞RGS5蛋白表达；并且GNP粒径越小,对周细胞影响越明显.当GNP粒径达到100 nm时,对周细胞增殖和抑制RGS5蛋白表达几乎无作用.
목적 관찰납미금(GNP)대인미혈관주세포증식화G단백신호조절단백5(RGSS)표체적영향.방법 제비농도위50 nmol/L직경분별위25、50、100 nm적GNP용액；체외배양인미혈관주세포주HBVP-1200；광학현미경관찰GNP작용주세포、재세포중침적；새서람(MTT)비색법검측주세포증식；원자력현미경( AFM)관찰주세포표면형모；Western blot검측주세포RGS5단백표체.결과 (1)불동조간적GNP( 25、50、100 nm)급대조조,기주세포증식솔분별위:(147.9±5.9)％、(121.7±3.4)％、(107.6±2.1)％급( 100.0±0.0)％；립경월소,주세포증식솔월고(P＜0.05).(2)AFM관찰도립경월소적GNP처리후,주세포표이형모변화월명현.주요표현재세포막내함、세포표면출현교대공동,유세포내탄현상. (3)Western blot검측도립경월소적GNP,억제RGS5단백표체월명현.결론 GNP촉진인미혈관주세포증식,억제주세포RGS5단백표체；병차GNP립경월소,대주세포영향월명현.당GNP립경체도100 nm시,대주세포증식화억제RGS5단백표체궤호무작용.
Objective To observe the effects of nangold(GNP) on proliferation and the expression of regulator of G-protein signaling5 (RGS5) of human microvascular pericytes.Methods The GNP solutions of 60 nmol/L with diameters of 25,50 and 100 nm were prepared.HBVP-1200 cells were cultured in vitro.The methyl thiazolium tetrazolium (MTT) was used to detect the effects of ditferent diameters of GNP on proliferation of pericytes.Atomic force microscope (AFM) was used to observe morphological changes of pericytes.The expression of RGS5 protein was detected by using Western blotting.Results GNP could euhance the proliferation of human microvascular pericytes.After treatment with different diameters of GNP (25,50,and 100 nm),the proliferation rate of pericytes was ( 147.9 ± 5.9) ％,( 121.7 ± 3.4) ％ and ( 107.6 ± 2.1 ) ％ respectively.When the diameter of GNP was smaller,the proliferation rate was increased significantly (P ＜0.05).Under AFM,the morphological changes of pericytes treated with GNP were observed,including invagination,obvious shrinked cell membrane and much rougher surface.The protein expression of RGS5 was reduced when the GNP was given 24 h later,especially when the diameter of GNP was 25 nm.Conclusion GNP could enhance the proliferation of human microvascular pericytes,and restrain the RGS5 protein expression.The smaller the diameter of GNP,the stronger effects of GNP on the pericytes.