肿瘤
腫瘤
종류
TUMOR
2001年
2期
85-87
,共3页
戴大英%翟为溶%万大方%朱腾方%叶圣龙
戴大英%翟為溶%萬大方%硃騰方%葉聖龍
대대영%적위용%만대방%주등방%협골룡
肝肿瘤%癌,肝细胞%基因产物,p21WAF1%基因产物,p53%免疫组化
肝腫瘤%癌,肝細胞%基因產物,p21WAF1%基因產物,p53%免疫組化
간종류%암,간세포%기인산물,p21WAF1%기인산물,p53%면역조화
目的探讨HCC中p21WAF1与p53的表达及其意义。方法应用免疫组化法检测38例手术切取的HCC及其配对癌旁肝组织、5例正常肝组织中p21WAF1和p53的表达，并分析它们与病理形态的关系。结果 p21WAF1、p53在HCC中的表达明显高于癌旁肝组织，p21WAF1阳性14例(36.8 %)，p53阳性28例(73.7 %)，其中两者表达一致(均阳或均阴)的18例，表达不一致的20例。癌旁肝组织1例(2.6 %)、正常肝组织2例p21WAF1呈阳性表达，所有癌旁及正常肝组织中均未见p53表达。HCC中p21WAF1表达与p53表达无明显关系(P＞0.05)。在不同组织学分级、有无肝内转移或门脉癌栓之间p21WAF1、p53表达差异均无显著性(P＞0.05)。结论p21WAF1与p53在癌内表达均高于癌旁，但两者间并无相关性，提示p21WAF1表达可能受p53依赖和p53非依赖性两种途径调节。
目的探討HCC中p21WAF1與p53的錶達及其意義。方法應用免疫組化法檢測38例手術切取的HCC及其配對癌徬肝組織、5例正常肝組織中p21WAF1和p53的錶達，併分析它們與病理形態的關繫。結果 p21WAF1、p53在HCC中的錶達明顯高于癌徬肝組織，p21WAF1暘性14例(36.8 %)，p53暘性28例(73.7 %)，其中兩者錶達一緻(均暘或均陰)的18例，錶達不一緻的20例。癌徬肝組織1例(2.6 %)、正常肝組織2例p21WAF1呈暘性錶達，所有癌徬及正常肝組織中均未見p53錶達。HCC中p21WAF1錶達與p53錶達無明顯關繫(P＞0.05)。在不同組織學分級、有無肝內轉移或門脈癌栓之間p21WAF1、p53錶達差異均無顯著性(P＞0.05)。結論p21WAF1與p53在癌內錶達均高于癌徬，但兩者間併無相關性，提示p21WAF1錶達可能受p53依賴和p53非依賴性兩種途徑調節。
목적탐토HCC중p21WAF1여p53적표체급기의의。방법응용면역조화법검측38례수술절취적HCC급기배대암방간조직、5례정상간조직중p21WAF1화p53적표체，병분석타문여병리형태적관계。결과 p21WAF1、p53재HCC중적표체명현고우암방간조직，p21WAF1양성14례(36.8 %)，p53양성28례(73.7 %)，기중량자표체일치(균양혹균음)적18례，표체불일치적20례。암방간조직1례(2.6 %)、정상간조직2례p21WAF1정양성표체，소유암방급정상간조직중균미견p53표체。HCC중p21WAF1표체여p53표체무명현관계(P＞0.05)。재불동조직학분급、유무간내전이혹문맥암전지간p21WAF1、p53표체차이균무현저성(P＞0.05)。결론p21WAF1여p53재암내표체균고우암방，단량자간병무상관성，제시p21WAF1표체가능수p53의뢰화p53비의뢰성량충도경조절。
Objective To explore the expression and significance of p21WAF1 and p53 in HCC. Methods Immunohistochemical method (IHC) was used to localize and semi-quantitate the proteins of p21WAF1 and p53 and to observe the relationship between the expression of p21WAF1 and the different histopathologic characters in 38 patients of HCC and their peri-cancer tissue as well as 5 normal liver tissue. Results Of all 38 cases, both p21WAF1 and p53 expression were significantly higher in tumor than that in corresponding non-tumors liver tissue; 14 (36.8 %) of 38 cases showed p21WAF1 positive staining, 28 cases (73.7 %) were p53 positive, p21WAF1＋/p53＋ or p21WAF1-/p53－ were observed in 18, while 20 cases showed p53＋/p21WAF1－ or p53－/p21WAF1＋. p21WAF1＋ was seen in 1 of 38 (2.6 %) corresponding non-cancerous tissue and 2 of 5 normal liver tissue. p53 protein was not detected neither in the non-tumorous tissue nor in normal liver. No significant association was found between the expressions of p21WAF1 and p53(P＞0.05) in HCC. Their was no significant correlation between p21WAF1 or p53 expression and the different histopathologic characters of tumor (differentiating grades, intrahepatic metastasis and/or cancerous thrombi within portal veins). Conclusion Both p21WAF1 and p53 proteins are over expressed in HCC than that in corresponding non-tumorous liver tissue, but there is no relationship between them. Both p53-independent and p53-dependent mechanism may play a role in regulating p21WAF1 expression in HCC. p21WAF1 immunostaining cannot be used to assess the status of p53 in any given cell or tissue.
