CAJ | 학술논문

背景:骨组织工程发展很快,但目前缺乏理想的支架材料,体外构建的组织工程化活性骨在体内的成骨能力各家报道不一.目的:探讨异种完全脱蛋白骨支架材料复合自体红骨髓体内植入的成骨作用.设计:随机对照的实验研究.单位:解放军济南军区第一五二医院骨科,平顶山市第一人民医院老干部病房,平顶山煤业集团朝川矿医院骨科.材料:实验在济南军区第一五二医院动物实验室完成.4月龄日本大耳白兔40只,体质量2.0～2 5 kg,雌雄不限(平顶山市第一五二医院动物实验室提供).干预:将经物理、化学方法处理制得的小牛完全脱蛋白骨(CFDB)支架材料与免自体红骨髓复合后植入免大腿肌肉内(40只)进行实验,术后4,8周分别进行检测.主要观察指标:①常规组织学检查.②碱性磷酸酶活性测定.③植入物成骨的定量判断.结果:实验组4周和8周移植物组织块ALP活性分别为(63.48±0.873)和(69.527±0.635)IU/L,移植物组织块成骨含量分析分别为(2.50±0.38)和(4.70±0.67)分,对照组4周和8周移植物组织块ALP活性分别为(2.50±0.38)和(4.70±0.67)IU/L,移植物组织块成骨含量分析分别为(1.90±0.54)和(3.40±0.54)分,表明,实验组在同时间点的成骨能力显著高于对照组,并随植入时间的延长,新骨形成增多.结论:异种完全脱蛋白骨支架材料可作为骨组织工程支架材料,复合自体红骨髓后植入体内,其成骨能力明显增加.
배경:골조직공정발전흔쾌,단목전결핍이상적지가재료,체외구건적조직공정화활성골재체내적성골능력각가보도불일.목적:탐토이충완전탈단백골지가재료복합자체홍골수체내식입적성골작용.설계:수궤대조적실험연구.단위:해방군제남군구제일오이의원골과,평정산시제일인민의원로간부병방,평정산매업집단조천광의원골과.재료:실험재제남군구제일오이의원동물실험실완성.4월령일본대이백토40지,체질량2.0～2 5 kg,자웅불한(평정산시제일오이의원동물실험실제공).간예:장경물리、화학방법처리제득적소우완전탈단백골(CFDB)지가재료여면자체홍골수복합후식입면대퇴기육내(40지)진행실험,술후4,8주분별진행검측.주요관찰지표:①상규조직학검사.②감성린산매활성측정.③식입물성골적정량판단.결과:실험조4주화8주이식물조직괴ALP활성분별위(63.48±0.873)화(69.527±0.635)IU/L,이식물조직괴성골함량분석분별위(2.50±0.38)화(4.70±0.67)분,대조조4주화8주이식물조직괴ALP활성분별위(2.50±0.38)화(4.70±0.67)IU/L,이식물조직괴성골함량분석분별위(1.90±0.54)화(3.40±0.54)분,표명,실험조재동시간점적성골능력현저고우대조조,병수식입시간적연장,신골형성증다.결론:이충완전탈단백골지가재료가작위골조직공정지가재료,복합자체홍골수후식입체내,기성골능력명현증가.
BACKGROUND: Although bone tissue engineering has been developed rapidly, ideal scaffold materials are deficient and the ability of tissue engineered bone constructed in vitro was reported inconsistently.OBJECTIVE: To study the ectopic osteogenesis of the implantation in vitro with composite fully deproteinized bone(CFDB) compounded by autologous red marrow.DESIGN: A randomized controlled study.SETTING: Department of Orthopaedics, the 152 Hospital of Jinan Military Area Command of Chinese PLA; Ward for Retired Cadres, First People's Hospital of Pingdingshan City; Department of Orthopaedics, Chaochuan Mine Hospital of Pingdingshan Coal Industrial Group.MATERIALS: The study was completed in the Department of Orthopaedics,the 152 Hospital of Jinan Militatry Area Command of Chinese PLA. Totally 40 Japanese flap-eared white rabbits of 4 months old of either gender with a body mass from 2.0 kg to 2.5 kg were involved (provided by the Laboratory for Experimental Animals of the 152 Hospital in Pingdingshan city).INTERVENTION: Calf CFDB scaffold materials were compounded by rabbit autologous red marrow after physical and chemical managements, which were then implanted into the thigh muscles of 40 rabbits. The osteogenetic abilities of the materials compounded by autologous red marrow were analyzed at 4 weeks and 8 weeks after operations respectively.ysis of the implanted bone.RESULTS: ALP activities were(63.48 ± 0. 873) and (69. 527 ± 0. 635) IU/L respectively, and the results of osteogenetic quantitative analysis were (2.50 ±0.38) and(4.70 ±0.67) points of rabbits in the study group at week 4 and week 8 respectively. ALP activities were(2.50±0.38) and (4.70 ± 0. 67) IU/L and the results of osteogenetic quantitative analysis were( 1.90 ± 0.54 ) and(3.40 ± 0.54) points of rabbits in the control group at week 4 and week 8 respectively. The results indicated that the osteogenetic ability of the study group was significantly higher than that of the control group at the same time point, and the neogenetic bone increased along with the prolongation of the implantation time.CONCLUSION: CFDB could be applied as scaffold materials for bone tissue engineering, and its osteogenesis increases significantly after being compounded by autologous red marrow.