CAJ | 학술논문

[目的] 寻求玉米秸秆预处理后的最佳酶解工艺条件.[方法] 采用碱浸泡法和氨水浸泡法对玉米秸秆进行预处理,考察预处理方法以及温度、酶用量、pH值、底物浓度等因素对玉米秸秆酶水解的影响,得出最佳酶解条件,并利用最佳条件下的水解液进行丁醇发酵.[结果] 碱法预处理玉米秸秆能有效地提高酶的水解效率.玉米秸秆经过预处理后的最佳酶水解工艺条件为:pH 值4.5～5.0,温度50 ℃,底物浓度3.33%,酶用量950 U/g 秸秆.利用秸秆水解液进行丁醇发酵后,溶剂(丁醇、丙酮、乙醇)的产率比值为10.0∶1.5∶1.0,与传统发酵(6∶3∶1)相比, 提高了丁醇所占的比值.[结论] 该研究为以木质纤维素为原料进行新能源的开发和利用验提供试验依据.
[목적] 심구옥미갈간예처리후적최가매해공예조건.[방법] 채용감침포법화안수침포법대옥미갈간진행예처리,고찰예처리방법이급온도、매용량、pH치、저물농도등인소대옥미갈간매수해적영향,득출최가매해조건,병이용최가조건하적수해액진행정순발효.[결과] 감법예처리옥미갈간능유효지제고매적수해효솔.옥미갈간경과예처리후적최가매수해공예조건위:pH 치4.5～5.0,온도50 ℃,저물농도3.33%,매용량950 U/g 갈간.이용갈간수해액진행정순발효후,용제(정순、병동、을순)적산솔비치위10.0∶1.5∶1.0,여전통발효(6∶3∶1)상비, 제고료정순소점적비치.[결론] 해연구위이목질섬유소위원료진행신능원적개발화이용험제공시험의거.
[Objective] The aim was to find the optimum enzymolysis process conditions after corn stalk pretreatment. [Method] The corn stalk was pretreated by using alkali steeping method and ammonia steeping method to study the effect of the factors such as pretreatment method, temperature, enzyme concentration, pH value and substrate concentration on the corn stalk enzymatic hydrolysis, which got the optimum enzymolysis process conditions and used the optimum condition hydrolysate to make butanol fermentation. [Result] The alkali method could effective improve the hydrolysis efficiency of enzyme. The optimum enzymatic hydrolysis process condition after corn stalk pretreatment was that the pH value was 4.5-5.0, the temperature was 50 ℃, the substrate concn. Was 3.33%, the enzyme dosage was 950 U/g stalk. The yield radio of solvents (butanol, acetone, ethanol) was 10.0∶1.5∶1.0 after making butanol fermentation by using stalk hydrolysate, which increased the radio of butanol, comparing with the traditional fermented(6∶3∶1). [Conclusion] This research provided the test basis for the development and utilization of new energy with lignocellulose as the material.