CAJ | 학술논문

目的:进一步探讨神经生长因子(NGF)/酪氨酸受体激酶A(TrkA)通路是否为龟板抗帕金森病(PD)大鼠多巴胺能神经元凋亡中的机制.方法:采用大鼠左侧黑质致密带注射6-羟基多巴胺(6-OHDA,0 2%)2μl造成PD模型,同时设立龟板组、模型组和正常对照组,用免疫组织化学显色方法观察PD大鼠中脑黑质NGF、 TrkA和磷酸化的糖原合成酶激酶-3β(p-GSK-3β)阳性神经元数目.免疫印迹法检测NGF、 TrkA、 p-GSK-3β蛋白表达水平的变化.结果:免疫组织化学显色显示龟板组PD大鼠中脑黑质致密部NGF、 TrkA的阳性细胞数明显多于模型组.免疫印迹法结果显示龟板组PD大鼠中脑黑质致密部NGF、 TrkA的蛋白表达水平高于模型组.结论:龟板能上调6-OHDA诱导的PD大鼠中脑黑质NGF、 TrkA和p-GSK-3β的表达,这可能是其抗PD大鼠多巴胺能神经元凋亡的分子机制.
목적:진일보탐토신경생장인자(NGF)/락안산수체격매A(TrkA)통로시부위구판항파금삼병(PD)대서다파알능신경원조망중적궤제.방법:채용대서좌측흑질치밀대주사6-간기다파알(6-OHDA,0 2%)2μl조성PD모형,동시설립구판조、모형조화정상대조조,용면역조직화학현색방법관찰PD대서중뇌흑질NGF、 TrkA화린산화적당원합성매격매-3β(p-GSK-3β)양성신경원수목.면역인적법검측NGF、 TrkA、 p-GSK-3β단백표체수평적변화.결과:면역조직화학현색현시구판조PD대서중뇌흑질치밀부NGF、 TrkA적양성세포수명현다우모형조.면역인적법결과현시구판조PD대서중뇌흑질치밀부NGF、 TrkA적단백표체수평고우모형조.결론:구판능상조6-OHDA유도적PD대서중뇌흑질NGF、 TrkA화p-GSK-3β적표체,저가능시기항PD대서다파알능신경원조망적분자궤제.
Objective:To explore whether NGF/TrkA pathway participates in antiapoptotic effect of plastrum testudinis (PT) on neurons of substantia nigra of Parkinson's disease (PD) rat induced by 6-hydroxydopamine (6-OHDA). Methods: Rat PD model was induced by injection of 6-OHDA (0. 2%, 2μl into the left compact zone of the substantia nigra. Rats were randomly divided into model control, normal control and PT treatment groups, which was administrated intragastrical-ly of 2g PT twice daily for 12 weeks. The number of NGF, TrkA and p-GSK-3β positive cells in substantia nigra was measured with SABC immunohistochemical method, and the expression of NGF, TrkA and p-GSK-3β was detected by Western blot Results:The number of NGF, TrkA and p-GSK-3β positive cells in substantia nigra was significantly increased in the PT treatment group compared with that in the model control group. The expression of NGF, TrkA and p-GSK-3β was also enhanced in the PT treatment group. Conclusion:PT can promote the expression of NGF, TrkA and pGSK-3β in the substantia nigra of PD rats induced by 6-OHDA, which may involve in its antiapoptotic effect in dopaminergic neurons of PD.