国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2011年
1期
14-16
,共3页
犬弓首线虫%诱导型一氧化氮合酶%TNF-α%协同表达%实时荧光定量PCR
犬弓首線蟲%誘導型一氧化氮閤酶%TNF-α%協同錶達%實時熒光定量PCR
견궁수선충%유도형일양화담합매%TNF-α%협동표체%실시형광정량PCR
Toxocara canis%Inducible nitric oxide synthase%TNF-α%Coexpression%Real-time fluorescent quantitative PCR
目的 观察感染犬弓首线虫小鼠肝组织中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和TNF-α表达的动态变化.方法 清洁级健康雌性昆明小鼠16只,随机分为正常对照组和犬弓首线虫感染3 d组、5 d组、9 d组(n=4),实验组用犬弓首线虫感染期虫卵灌胃感染,对照组小鼠以等量无菌生理盐水灌胃.感染后第3、5、9天剖杀小鼠,取其适量肝组织,以RNAiso试剂提取肝组织总RNA,实时荧光定量RT-PCR方法检测肝组织iNOS和TNF-α表达量.结果 对照组小鼠肝脏中未检测到iNOS的转录表达,实验组犬弓首线虫感染后第3天检测到iNOS的表达,感染后第5、9天肝组织内iNOS表达量逐渐降低.对照组和犬弓首线虫感染后不同时间小鼠的肝组织内均可检测到TNF-α的转录表达,感染后第3天表达显著增强且达峰值,第5、9天表达量逐渐降低.结论 犬弓首线虫感染可诱导iNOS和TNF-α在肝组织的协同表达,且二者表达的动态变化趋势一致.
目的 觀察感染犬弓首線蟲小鼠肝組織中誘導型一氧化氮閤酶(inducible nitric oxide synthase,iNOS)和TNF-α錶達的動態變化.方法 清潔級健康雌性昆明小鼠16隻,隨機分為正常對照組和犬弓首線蟲感染3 d組、5 d組、9 d組(n=4),實驗組用犬弓首線蟲感染期蟲卵灌胃感染,對照組小鼠以等量無菌生理鹽水灌胃.感染後第3、5、9天剖殺小鼠,取其適量肝組織,以RNAiso試劑提取肝組織總RNA,實時熒光定量RT-PCR方法檢測肝組織iNOS和TNF-α錶達量.結果 對照組小鼠肝髒中未檢測到iNOS的轉錄錶達,實驗組犬弓首線蟲感染後第3天檢測到iNOS的錶達,感染後第5、9天肝組織內iNOS錶達量逐漸降低.對照組和犬弓首線蟲感染後不同時間小鼠的肝組織內均可檢測到TNF-α的轉錄錶達,感染後第3天錶達顯著增彊且達峰值,第5、9天錶達量逐漸降低.結論 犬弓首線蟲感染可誘導iNOS和TNF-α在肝組織的協同錶達,且二者錶達的動態變化趨勢一緻.
목적 관찰감염견궁수선충소서간조직중유도형일양화담합매(inducible nitric oxide synthase,iNOS)화TNF-α표체적동태변화.방법 청길급건강자성곤명소서16지,수궤분위정상대조조화견궁수선충감염3 d조、5 d조、9 d조(n=4),실험조용견궁수선충감염기충란관위감염,대조조소서이등량무균생리염수관위.감염후제3、5、9천부살소서,취기괄량간조직,이RNAiso시제제취간조직총RNA,실시형광정량RT-PCR방법검측간조직iNOS화TNF-α표체량.결과 대조조소서간장중미검측도iNOS적전록표체,실험조견궁수선충감염후제3천검측도iNOS적표체,감염후제5、9천간조직내iNOS표체량축점강저.대조조화견궁수선충감염후불동시간소서적간조직내균가검측도TNF-α적전록표체,감염후제3천표체현저증강차체봉치,제5、9천표체량축점강저.결론 견궁수선충감염가유도iNOS화TNF-α재간조직적협동표체,차이자표체적동태변화추세일치.
Objective To observe the time-course change in expression of inducible nitric oxide synthase(iNOS) and tumor necrosis factor-α(TNF-α) in the liver of mice infected with Toxocara canis. Methods The livers of 16 healthy Kunming female mice were collected uninfected or infected with T. canis on day 3, 5, 9 post infection( p.i.) , totle RNA of livers were extracted with RNAiso reagent, then time-course change of the mRNA expression of iNOS and TNF-α was detected by real time fluorescent quantitative RT-PCR.Results There was no expression of iNOS in the livers of mice uninfected. The mRNA expression of iNOS increased significantly and reached peak at day 3 p. i. , then the expression decreased gradually on the day 5 and 9 p. i.. The mRNA expression of TNF-α was detectable in the livers of both infected and uninfected mice,and the pattern of time-course change was same as that of iNOS. Conclusion The infection of T. canis can induce coexpression of iNOS and TNF-α in the liver of mice, both iNOS and TNF-α had the same pattern of time-course change of expression.
