中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2008年
12期
913-917
,共5页
王立芝%谭冬梅%尉晓蔚%彭洪英%赖国旗%谭毅
王立芝%譚鼕梅%尉曉蔚%彭洪英%賴國旂%譚毅
왕립지%담동매%위효위%팽홍영%뢰국기%담의
肿瘤转移%肿瘤侵润%分泌型卷曲相关蛋白2
腫瘤轉移%腫瘤侵潤%分泌型捲麯相關蛋白2
종류전이%종류침윤%분비형권곡상관단백2
Neoplasm metastasis%Neoplasm invasiveness%Secreted frizzled-related protein 2
目的 了解分泌型卷曲相关蛋白2(sFRP2)对HepG2细胞增殖、侵袭和迁移等生物学行为的影响.方法 采用sFRP2重组腺病毒感染HepG2细胞,四甲基偶氮唑盐法检测HepG2细胞增殖,流式细胞术检测细胞周期分布,免疫组织化学法检测肿瘤转移相关因子的表达,Westernblot检测β连环素的表达,Tmnswell小室检测细胞迁移能力.结果 sFRP2明显抑制HepG2细胞增殖,限制细胞周期从Go/G<,1>期进入S期;sFRP2显著增强nepG2细胞CD44和CD82/KAI1等与肿瘤转移抑制相关蛋白的表达,而明显降低有助于肿瘤侵袭转移的细胞外基质金属蛋白酶诱导因子的表达; sFRP2可降低HepG2细胞的迁移能力.sFRP2感染前后,HepG2细胞均有β连环素的表达,且其表达差异无统计学意义.结论 sFRP2的重组腺病毒能成功感染HepG2细胞,并对H印G2细胞的增殖、侵袭和转移具有抑制效应.
目的 瞭解分泌型捲麯相關蛋白2(sFRP2)對HepG2細胞增殖、侵襲和遷移等生物學行為的影響.方法 採用sFRP2重組腺病毒感染HepG2細胞,四甲基偶氮唑鹽法檢測HepG2細胞增殖,流式細胞術檢測細胞週期分佈,免疫組織化學法檢測腫瘤轉移相關因子的錶達,Westernblot檢測β連環素的錶達,Tmnswell小室檢測細胞遷移能力.結果 sFRP2明顯抑製HepG2細胞增殖,限製細胞週期從Go/G<,1>期進入S期;sFRP2顯著增彊nepG2細胞CD44和CD82/KAI1等與腫瘤轉移抑製相關蛋白的錶達,而明顯降低有助于腫瘤侵襲轉移的細胞外基質金屬蛋白酶誘導因子的錶達; sFRP2可降低HepG2細胞的遷移能力.sFRP2感染前後,HepG2細胞均有β連環素的錶達,且其錶達差異無統計學意義.結論 sFRP2的重組腺病毒能成功感染HepG2細胞,併對H印G2細胞的增殖、侵襲和轉移具有抑製效應.
목적 료해분비형권곡상관단백2(sFRP2)대HepG2세포증식、침습화천이등생물학행위적영향.방법 채용sFRP2중조선병독감염HepG2세포,사갑기우담서염법검측HepG2세포증식,류식세포술검측세포주기분포,면역조직화학법검측종류전이상관인자적표체,Westernblot검측β련배소적표체,Tmnswell소실검측세포천이능력.결과 sFRP2명현억제HepG2세포증식,한제세포주기종Go/G<,1>기진입S기;sFRP2현저증강nepG2세포CD44화CD82/KAI1등여종류전이억제상관단백적표체,이명현강저유조우종류침습전이적세포외기질금속단백매유도인자적표체; sFRP2가강저HepG2세포적천이능력.sFRP2감염전후,HepG2세포균유β련배소적표체,차기표체차이무통계학의의.결론 sFRP2적중조선병독능성공감염HepG2세포,병대H인G2세포적증식、침습화전이구유억제효응.
Objective To investigate the effect of sFRP2 on the biological behavior of human hepatoma carcinoma HepG2 cells. Methods HepG2 cells were infected with recombinant adenovirus con-raining mouse sFRP2 gene, and then the proliferation, cell cycle distribution, expression of tumor metastasis related factors (CD44, CD82/KAII, EMMPRIN) and beta-catenin protein, and migration abifity of the cells were detected by MTT, FCM, immunohistochemistry, Western blot and Transwell inserts, respectively. Re-sults sFRP2 protein inhibited the proliferation of HepG2 cells, and increased the percentage of G<,0>/G<,1> period cells. Expression of CD44 and CD82/KAI1 proteins, which could inhibit invasion and metastasis of tumor cells, was upregulated. However, EMMPRIN protein, which could promote the above properties of tumor cells decreased in HepG2 cells infected with the recombinant adenovirus containing mouse sFRP-2 gene. Western blot demonstrated that bcta-catenin was expressed in HepG2 cells and there was no significant differ-ence between the treated and the control groups. Transwell insert test showed sFRP2 protein decreased the migration ability of HepG2 cells. Conclusion The recombinant adenovirus containing mouse sFRP-2 gene could infect HepG2 cells, sFRP2 protein could significantly reduce the capability of proliferation, invasion and metastasis of HepG2 celts.
