CAJ | 학술논문

目的研究丙型肝炎患者树突状细胞表面特异性非整联蛋白同源物(DC-SIGNR)外显子4基因颈区重复序列的遗传多态性分布,探讨DC-SIGNR基因多态性与HCV病毒基因分型及HCV载量的关系. 方法采用PCR结合DNA测序对268例丙型肝炎患者DC-SIGNR重复序列多态性进行基因分型和测序分析,同时检测患者的HCV病毒载量及HCV病毒基因分型.组间两两比较用LSD法.结果 DC-SIGNR基因型或等位基因与HCV基因分型的相关性不显著.HCV载量携带7等位基因的患者为(4.6722±1.9766)log10拷贝/ml,携带9等位基因的患者为(5.3073±1.6795)log10拷贝/ml,P=0.036,两组差异有统计学意义.7/7基因型的患者HCV载量水平为(4.5974±2.0067)log10拷贝/ml,9/7基因型的患者组为(5.2771±1.8587)log10拷贝/ml,P=0.025,两组差异有统计学意义.提示HCV更易与携带较长DC-SINGR等位基因的患者结合.结论 DC-SIGNR遗传多态性可能与HCV在个体内的复制有关,但与HCV基因分型不相关.
목적 연구병형간염환자수돌상세포표면특이성비정련단백동원물(DC-SIGNR)외현자4기인경구중복서렬적유전다태성분포,탐토DC-SIGNR기인다태성여HCV병독기인분형급HCV재량적관계. 방법채용PCR결합DNA측서대268례병형간염환자DC-SIGNR중복서렬다태성진행기인분형화측서분석,동시검측환자적HCV병독재량급HCV병독기인분형.조간량량비교용LSD법.결과 DC-SIGNR기인형혹등위기인여HCV기인분형적상관성불현저.HCV재량휴대7등위기인적환자위(4.6722±1.9766)log10고패/ml,휴대9등위기인적환자위(5.3073±1.6795)log10고패/ml,P=0.036,량조차이유통계학의의.7/7기인형적환자HCV재량수평위(4.5974±2.0067)log10고패/ml,9/7기인형적환자조위(5.2771±1.8587)log10고패/ml,P=0.025,량조차이유통계학의의.제시HCV경역여휴대교장DC-SINGR등위기인적환자결합.결론 DC-SIGNR유전다태성가능여HCV재개체내적복제유관,단여HCV기인분형불상관.
Objective To investigate the association of genetic polymorphism of dendritic cellspecific ICAM-grabbing nonintegrin(DC-SIGNR)and hepatitis C infection.Methods Patients with hepatitis C(n=268)were genotyped and analysed for the repeat sequences polymorphism of DC-SIGNR using PCR and DNA sequencing.HCV virus load and HCV RNA genotypes were analyzed.Inter-group comparison was analyzed using LSD method.Results No significant correlation was found between DC-SIGNR genotypes/alleles and HCV RNA genotypes in patients.HCV-infected patients with 7-repeat(medium)alleles had lower HCV RNA levels compared to patients with 9-repeat(onger)alleles(P=0.036).HCV-infected patients with 7/7 genotype had lower HCV RNA levels compared to patients with 9/7 genotype(P=0.025).These findings suggest that optimal attachment of hepatitis C virions to DC-SIGNR may be associated with longer alleles.Conclusion The fact that DC-SIGNR polymorphism might affect HCV loads suppons the concept that DC-SIGNR contributes to HCV replication efficacy.There is no significant correlation between the genetic polymorphism of DC-SIGNR and HCV-RNA genotypes.