中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
3期
191-194
,共4页
温焕连%黎喜梅%曾文铤%朱科伦
溫煥連%黎喜梅%曾文鋌%硃科倫
온환련%려희매%증문정%주과륜
人参皂苷Rg3%抗药性,肿瘤%肝肿瘤%多药耐药相关蛋白类
人參皂苷Rg3%抗藥性,腫瘤%肝腫瘤%多藥耐藥相關蛋白類
인삼조감Rg3%항약성,종류%간종류%다약내약상관단백류
Ginsenoside Rg3%Drug resistance,neoplasms%Live neoplasms%Multidrug resistance-association proteins
目的 观察人参皂苷Rg3对逆转人肝癌细胞BEL-7402耐药作用.方法 MTT法测定人参皂苷Rg3与长春新碱(VCR)联合对肝癌细胞BEL-7402生长抑制率,反转录多聚酶链反应(RT-PCR)检测人参皂苷Rg3作用于肝癌细胞MDRImRNA的表达量.结果 不同浓度的人参皂苷Rg3( 10、20、30、40、60 mg/L)与长春新碱(0.02 mg/L)联合作用后,其对细胞增殖抑制率较单独用药时作用增加(P＜0.05),相互作用指数CDI＜1.人参皂苷Rg3 40.0μg/mL作用于肝癌细胞BEL-7402不同时间(24、48、72h)后MDR1mRNA的表达量明显下降[(79.21±2.23)％,(62.58±2.46)％,(45.46±1.13)％],与对照组差异有统计学意义(P＜0.05).结论 人参皂苷Rg3与长春新碱联合应用具有协同抑制作用,能逆转BEL7402对VCR的耐药.人参皂苷Rg3能抑制BEL-7402细胞MDR1 mRNA的表达.
目的 觀察人參皂苷Rg3對逆轉人肝癌細胞BEL-7402耐藥作用.方法 MTT法測定人參皂苷Rg3與長春新堿(VCR)聯閤對肝癌細胞BEL-7402生長抑製率,反轉錄多聚酶鏈反應(RT-PCR)檢測人參皂苷Rg3作用于肝癌細胞MDRImRNA的錶達量.結果 不同濃度的人參皂苷Rg3( 10、20、30、40、60 mg/L)與長春新堿(0.02 mg/L)聯閤作用後,其對細胞增殖抑製率較單獨用藥時作用增加(P＜0.05),相互作用指數CDI＜1.人參皂苷Rg3 40.0μg/mL作用于肝癌細胞BEL-7402不同時間(24、48、72h)後MDR1mRNA的錶達量明顯下降[(79.21±2.23)％,(62.58±2.46)％,(45.46±1.13)％],與對照組差異有統計學意義(P＜0.05).結論 人參皂苷Rg3與長春新堿聯閤應用具有協同抑製作用,能逆轉BEL7402對VCR的耐藥.人參皂苷Rg3能抑製BEL-7402細胞MDR1 mRNA的錶達.
목적 관찰인삼조감Rg3대역전인간암세포BEL-7402내약작용.방법 MTT법측정인삼조감Rg3여장춘신감(VCR)연합대간암세포BEL-7402생장억제솔,반전록다취매련반응(RT-PCR)검측인삼조감Rg3작용우간암세포MDRImRNA적표체량.결과 불동농도적인삼조감Rg3( 10、20、30、40、60 mg/L)여장춘신감(0.02 mg/L)연합작용후,기대세포증식억제솔교단독용약시작용증가(P＜0.05),상호작용지수CDI＜1.인삼조감Rg3 40.0μg/mL작용우간암세포BEL-7402불동시간(24、48、72h)후MDR1mRNA적표체량명현하강[(79.21±2.23)％,(62.58±2.46)％,(45.46±1.13)％],여대조조차이유통계학의의(P＜0.05).결론 인삼조감Rg3여장춘신감연합응용구유협동억제작용,능역전BEL7402대VCR적내약.인삼조감Rg3능억제BEL-7402세포MDR1 mRNA적표체.
Objective To determine the effect of ginsenoside Rg3 on reversal of multidrug resistance of hepatocellular carcinoma (HCC) cells bel-7402.Methods MTT method was employed to determine the inhibitory rate of ginsenoside Rg3 in combination with vincristine(VCR) on human HCC cells bel-7402.The cffcct of ginscnoside Rg3 on MDR1 mRNA cxpression in HCC cells was detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results The ginsenoside Rg3 of various concentrations ( 10,20,30,40,60 mg/L) combined with VCR(0.02 mg/L) produced longer inhibitory action than either drug alone (all P＜0.05,CDI＜1).Ginsenoside Rg3 (40.0 mg/L) led to marked reduction in MDR1 mRNA expression[ (79.21±2.23)％,(62.58±2.46％,(45.46±1.13％) ] in HCC cells bel-7402 at various time points (24 h,48 h and 72 h) as compared with control group (all P＜0.05).Conclusion Charactcrizcd by inhibiting MDR1 mRNA expression in bel-7402 cells,ginsenoside Rg3 combined with VCR has co-inhibition on reversal of HCC cells bel-7402 resistance to VCR.
