CAJ | 학술논문

目的研究131I-Herceptin荷人乳腺癌裸鼠体内的生物学分布及荷人乳腺癌裸鼠的放射免疫显像特点.方法以对数生长期的SK-BR-3乳腺癌细胞皮下接种BALB/c-neu裸鼠建立动物模型,对荷瘤小鼠模型进行SPECT连续显像.测量小鼠注药后的4、12、24、48 h各脏器每克组织每分钟的放射性计数(cpm/g),并计算T/NT以及每克组织的放射性计数占注射剂量放射性计数的百分比(％ ID/g).结果 (1)SK-BR-3细胞皮下接种BALB/c-neu裸鼠后成瘤率96％.(2)实验组对比对照组,显像对比明显；实验组T/NT以及肿瘤组织％ID/g显著高于对照组(P＜0.01).结论 131I-Herceptin在SK-BR-3乳腺癌裸鼠肿瘤组织中的浓聚明显,具有良好的靶向作用.
목적 연구131I-Herceptin하인유선암라서체내적생물학분포급하인유선암라서적방사면역현상특점.방법 이대수생장기적SK-BR-3유선암세포피하접충BALB/c-neu라서건립동물모형,대하류소서모형진행SPECT련속현상.측량소서주약후적4、12、24、48 h각장기매극조직매분종적방사성계수(cpm/g),병계산T/NT이급매극조직적방사성계수점주사제량방사성계수적백분비(％ ID/g).결과 (1)SK-BR-3세포피하접충BALB/c-neu라서후성류솔96％.(2)실험조대비대조조,현상대비명현；실험조T/NT이급종류조직％ID/g현저고우대조조(P＜0.01).결론 131I-Herceptin재SK-BR-3유선암라서종류조직중적농취명현,구유량호적파향작용.
Objective To study the biologic distribution of 131I-Herceptin in BALB/c-neu nude mice bearing HER-2 positive SK-BR-3 human breast cancer xenografts and the radioimmunoimaging characteristics of nude mouse bearing human SK-BR-3 breast cancer xenografts. Method SK-BR-3 breast cancer cells were implanted subcutaneously to athymic mice to establish animal model.Tumor bearing mice were continuously imaged with SPECT. The radiocounting per minute (cpm) of different organ on a γ-arithmometer was measured at 4,12,24,48 h postinjection of 131I-Herceptin or 131I-mlgG,and the T/NT ratios and the uptake percentages per gram of the injection dose (％ ID/g) was gained. Results Model was established in 96％ nude mouse.Compared with the control group,there was a significantly stronger contrast enhancement of tumor imaging,bigger T/NT and ％ ID/g in experimental group ( P ＜ 0.0l ).Conclusions 131I-Herceptin concentrates obviously in implanting tumor tissues of nude mouse,hence it is a good radiopharmaceutical agent targeting SK-BR-3 xenografts.