CAJ | 학술논문

目的研究骨髓增殖性肿瘤(MPN)患者骨髓间充质干细胞(BMSC)的生物学特性,检测其BMSC是否存在JAK2基因突变.方法体外分离培养健康志愿者和初诊MPN患者BMSC,光学显微镜下行细胞形态学观察;流式细胞术鉴定BMSC表面标志;检测BMSC成脂、成骨、成软骨分化能力;检测MPN患者BMSC以及外周血或骨髓单个核细胞是否存在JAK2 V617F、JAK2基因12号外显子(JAK2 exon12)突变.结果 MPN患者的BMSC细胞形态、表面标志、分化能力与正常对照相比无明显差异.检测到2l例真性红细胞增多症(PV)、17例原发性血小板增多症(ET)及1例原发性骨髓纤维化(PMF)患者外周血(或骨髓)标本存在JAK2 V617F突变,但未发现其BMSC存在该突变.检测到2例Pv和1例ET患者外周血(或骨髓)标本存在JAK2 exon 12突变,但未发现其BMSC存在该突变.结论 MPN患者BMSC与健康志愿者BMSC的生物学特性无明显差异;在外周血或骨髓单个核细胞JAK2基因突变阳性及阴性的MPN患者中,均未检测到其BMSC存在该突变.
목적 연구골수증식성종류(MPN)환자골수간충질간세포(BMSC)적생물학특성,검측기BMSC시부존재JAK2기인돌변.방법 체외분리배양건강지원자화초진MPN환자BMSC,광학현미경하행세포형태학관찰;류식세포술감정BMSC표면표지;검측BMSC성지、성골、성연골분화능력;검측MPN환자BMSC이급외주혈혹골수단개핵세포시부존재JAK2 V617F、JAK2기인12호외현자(JAK2 exon12)돌변.결과 MPN환자적BMSC세포형태、표면표지、분화능력여정상대조상비무명현차이.검측도2l례진성홍세포증다증(PV)、17례원발성혈소판증다증(ET)급1례원발성골수섬유화(PMF)환자외주혈(혹골수)표본존재JAK2 V617F돌변,단미발현기BMSC존재해돌변.검측도2례Pv화1례ET환자외주혈(혹골수)표본존재JAK2 exon 12돌변,단미발현기BMSC존재해돌변.결론 MPN환자BMSC여건강지원자BMSC적생물학특성무명현차이;재외주혈혹골수단개핵세포JAK2기인돌변양성급음성적MPN환자중,균미검측도기BMSC존재해돌변.
Objective To study the biological characteristics of bone marrow mesenchymal stem cells ( BMSCs) and detect JAK2 mutation in BMSCs from myeloproliferative neoplasms (MPN) patients. Methods JAK2 V617F mutation and exon 12 mutation in 70 MPN patients' blood or bone marrow samples were detected. Isolated BMSCs were then characterized their phenotype, mesenchymal differentiation capacity and existence of JAK2 mutation. Results BMSCs derived from the patients were similar with healthy donors in terms of morphology, surface antigen and differentiation ability. Of them, 38 patients' blood or bone marrow samples harbored JAK2 V617F, and identified that 3 V617F-negative-patients' samples existed JAK2 exon 12. No patients' BMSC harbored JAK2 mutation though their blood or bone marrow samples carried JAK2 mutation. Conclusion BMSCs from MPN patients had similar biological characteristics with healthy donors. BMSCs from MPN patients known to bear JAK2 mutation in blood or bone marrow cells didn' t carry the mutation.