CAJ | 학술논문

[目的]探讨不同浓度维生素C(VitC)对3种粪便隐血试验(FOBT)检测方法的影响.[方法]以蒸馏水为对照,分别用联苯胺法、匹拉米洞法和胶体金法检测含有不同浓度VitC的粪便隐血阴性、阳性标本.[结果]体外试验显示,当VitC浓度分别为5、75、100、125、250 mg/ml时,对3种方法检测FOBT阴性标本均不造成假阳性[结果].VitC浓度为5、125、250 mg/ml时,分别对联苯胺法、匹拉米洞法检测血红蛋白含量0.2～10、50～75、125 mg/ml的阳性标本造成假阴性影响,且含血红蛋白量越多,VitC越不容易造成假阴性的影响；而对胶体金法无影响.[结论]体外实验显示不同浓度VitC,对联苯胺法、匹拉米洞法检测粪便隐血阴性标本不产生假阳性影响,对阳性标本会造成假阴性影响；对胶体金法无影响.
[목적]탐토불동농도유생소C(VitC)대3충분편은혈시험(FOBT)검측방법적영향.[방법]이증류수위대조,분별용련분알법、필랍미동법화효체금법검측함유불동농도VitC적분편은혈음성、양성표본.[결과]체외시험현시,당VitC농도분별위5、75、100、125、250 mg/ml시,대3충방법검측FOBT음성표본균불조성가양성[결과].VitC농도위5、125、250 mg/ml시,분별대련분알법、필랍미동법검측혈홍단백함량0.2～10、50～75、125 mg/ml적양성표본조성가음성영향,차함혈홍단백량월다,VitC월불용역조성가음성적영향；이대효체금법무영향.[결론]체외실험현시불동농도VitC,대련분알법、필랍미동법검측분편은혈음성표본불산생가양성영향,대양성표본회조성가음성영향；대효체금법무영향.
[Objective]To investigate the effect of different contents of vitamin C on the three test [Methods]of fecal occult blood test(FOBT).[Methods]On the contrast of distilled water,Benzidine,Pyramidon,Colloidal gold were used to test samples with positive and negative result of FOBT with different contents of vitamin C.[Results]In vitro tests,there was no pseudo-positive result in negative samplesof FOBT with 5,75,100,125,250 mg/ml of vitamin C by the three different test [Methods]; however,when using positive samples of FOBT,pseudo-negative results were found by the [Methods]of Benzidine and Pyramidon when the contents of vitamin C were 5,125,250 mg/ml and the contents of hemoglobinwere 0.2-10,50-75,125 mg/ml.What's more,it was not so easy for the vitamin C to influence the positive sample when the hemoglobin becomes more and more,there was no effect on the method of colloidal gold.[Conclusions]In vitro tests,for the [Methods]of Benzidine and Pyramidon,it has no influence on negative samples of FOBT and it can result in pseudo-negative result when using positive samples of FOBT when the content of vitamin C is smaller than or equal to 250 mg/ml; For the method of Colloidal gold,it has no influence on negative and positive samples of FOBT with different density of vitamin C.