CAJ | 학술논문

目的选择性雌激素受体调节剂对去势大鼠松质骨、皮质骨骨密度的影响. 方法将21只SD大鼠随机分为3组，每组7只，A组为对照组，B组为去势组，C组为去势+他莫昔芬组，在去势后5 wk给药. 术后16 wk处死各组大鼠，进行离体骨密度（bone mineral density， BMD）测量及组织形态学测量. 结果①与对照组相比，去势组去势后16 wk股骨头、股骨颈、股骨干、肱骨头、肱骨干、腰椎BMD明显降低(P<0.01); 骨小梁体积、骨小梁厚度、椎体皮质骨厚度、结连结密度、结密度亦明显降低（P<0.05）而游离端密度和结连游离端密度增高（P<0.01）；②他莫昔芬治疗后11 wk，各部位BMD增加（P<0.05）但没有恢复到正常水平（P<0.05）；腰椎骨小梁连接性参数恢复到对照组水平（P>0.05）. 结论①去势后16 wk大鼠松质骨、皮质骨骨量均明显下降，腰椎骨小梁三维结构破坏，骨小梁连结程度降低；②他莫昔芬治疗后，松质骨、皮质骨骨量均明显增加，但没有恢复到去势前水平；③他莫昔芬能增加腰椎椎体皮质骨厚度，增加腰椎骨小梁厚度. 增加皮质骨、松质骨骨量；还可提高腰椎微构筑的质量，使骨小梁连结程度增加.
목적선택성자격소수체조절제대거세대서송질골、피질골골밀도적영향. 방법장21지SD대서수궤분위3조，매조7지，A조위대조조，B조위거세조，C조위거세+타막석분조，재거세후5 wk급약. 술후16 wk처사각조대서，진행리체골밀도（bone mineral density， BMD）측량급조직형태학측량. 결과①여대조조상비，거세조거세후16 wk고골두、고골경、고골간、굉골두、굉골간、요추BMD명현강저(P<0.01); 골소량체적、골소량후도、추체피질골후도、결련결밀도、결밀도역명현강저（P<0.05）이유리단밀도화결련유리단밀도증고（P<0.01）；②타막석분치료후11 wk，각부위BMD증가（P<0.05）단몰유회복도정상수평（P<0.05）；요추골소량련접성삼수회복도대조조수평（P>0.05）. 결론①거세후16 wk대서송질골、피질골골량균명현하강，요추골소량삼유결구파배，골소량련결정도강저；②타막석분치료후，송질골、피질골골량균명현증가，단몰유회복도거세전수평；③타막석분능증가요추추체피질골후도，증가요추골소량후도. 증가피질골、송질골골량；환가제고요추미구축적질량，사골소량련결정도증가.
AIM　To discuss the influence of selective estrogen receptor modulator (SERM) on bone mineral density loss in cancellated bone and compact bone of ovariectomized rats. METHODS　Twenty-one female SD rats were divided into three groups (7 rats per group). Bilateral ovariectomies were done on group B and C. Sham operation was done on group A. Group C was treated with tamoxifen 5 weeks later. Sixteen weeks after operation, all rats were sacrificed. BMD of L1-L5 vertebrae, femoral head, femoral neck, femoral body, humeral head and humeral body were measured by dual energy x-ray absorptiometry (DEXA), and L2 Vertebrae were for histomorphometric analysis. RESULTS　① Sixteen weeks after ovariectomy, BMD of L1-L5 vertebrae, femoral head, femoral neck, femoral body, humeral head, humeral body decreased significantly （P<0.01）, while the density of free endpoints and free-notes struts increased significantly （P<0.01） as compared with sham group. The trabecular became thiner and irregular which changed the bone microstructure in three dimensions. ② After treatment of tamoxifen, the above parameters restored (P<0.05), but not to the level of sham group (P>0.05). CONCLUSION　① Ovariectomy may induce significant decrease of cancellated bone mass and compacts bone mass. ② Treatment tamoxifen could inhibit bone loss, but the bone mass couldn't restore to the level of sham group.