CAJ | 학술논문

报道了仙鹤藓(Atrichum undulatum(Hedw.)P.Beauv.)和仙鹤藓小形变种(Atrichum undulatum var.minus(Hedw.)Par.)的组织培养再生体系的建立.为研究仙鹤藓属(Atrichum)藓类愈伤组织的诱导和再分化,将仙鹤鲜和仙鹤藓小形变种原丝体接种在含有4%葡萄糖和0.2～2.0 mg/L 6-BA的MS培养基上,培养一个月后,成功地诱导出疏松、易碎的绿色愈伤组织.愈伤组织诱导和常规继代培养较适合的培养基为含4%葡萄糖和1～2 mg/L6-BA的MS培养基.当将继代培养5次的脱分化藓类愈伤组织转移到含4%葡萄糖但无任何激素的MS培养基上时,能再分化形成原丝体,而在无任何碳源的Benecke培养基上培养时,能再分化形成经原丝体阶段发育来的直立配子体.
보도료선학선(Atrichum undulatum(Hedw.)P.Beauv.)화선학선소형변충(Atrichum undulatum var.minus(Hedw.)Par.)적조직배양재생체계적건립.위연구선학선속(Atrichum)선류유상조직적유도화재분화,장선학선화선학선소형변충원사체접충재함유4%포도당화0.2～2.0 mg/L 6-BA적MS배양기상,배양일개월후,성공지유도출소송、역쇄적록색유상조직.유상조직유도화상규계대배양교괄합적배양기위함4%포도당화1～2 mg/L6-BA적MS배양기.당장계대배양5차적탈분화선류유상조직전이도함4%포도당단무임하격소적MS배양기상시,능재분화형성원사체,이재무임하탄원적Benecke배양기상배양시,능재분화형성경원사체계단발육래적직립배자체.
In vitro regeneration systems of Atrichum mosses, Atrichum undulatum (Hedw.) P. Beauv. andA. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli wereinduced successfully from inoculated protonema of Atrichum mosses on MS medium containing glucose(4%) and 6-BA (0.2-2.0 mg/L). The suitable culture medium for the callus induction and regular subculturewas MS medium with 1.0-2.0 mg/L 6-BA and 4% glucose. The calli of Atrichum mosses developed intoprotonema, when it was transferred to phytohormone-free MS medium with 4% glucose. Meanwhile, thecalli developed into erect gametophytes through protonema phase on carbohydrate-free Benecke medium.