一种有效的红树根部总RNA提取方法
일충유효적홍수근부총RNA제취방법
An Efficient Method for Preparation of the Total RNA from the Root of Mangrove
  • 万方数据
    华北农学报 화북농학보
    ACTA AGRICULTURAE BOREALI-SINICA
    2009年 z2期 13-15 ,共3页

    符秀梅%韩淑梅%朱红林%周秀%李小靖%吴辉%谢俊%陈银华

    부수매%한숙매%주홍림%주수%리소정%오휘%사준%진은화

    红树%RNA提取%CTAB法 紅樹%RNA提取%CTAB法
    홍수%RNA제취%CTAB법
    Mangrove%Preparation of RNA%Method of CTAB
    红树作为一种重要的耐盐植物,为耐盐研究提供了重要的基因资源.本研究分别采用SDS、TRIZOL、CTAB等3种不同的方法进行了红树RNA提取,经电泳分析、紫外吸收检测、cDNA合成等技术手段检测表明,改进的CTAB法所提取的红树总RNA质量最好.
    홍수작위일충중요적내염식물,위내염연구제공료중요적기인자원.본연구분별채용SDS、TRIZOL、CTAB등3충불동적방법진행료홍수RNA제취,경전영분석、자외흡수검측、cDNA합성등기술수단검측표명,개진적CTAB법소제취적홍수총RNA질량최호.
    The mangrove is an important salt-tolerance plant. In this research, three method for isolation of total RNA from the root of mangrove were done. The improved method of CTAB was verified to be the most efficient method through the way of the electrophoretic patterns of nucleic acids and absorbanee at 230,260 and 280 nm in a UV-Vis spectrophotometer.
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