CAJ | 학술논문

以酿酒酵母(Saccharomyces cerevisiae AS2.1392)全细胞为催化剂不对称还原苯甲酰甲酸甲酯合成(R)-扁桃酸甲酯,该催化剂催化速度快、操作稳定性好.研究了底物和产物浓度对反应初速度的影响,建立了底物和产物抑制模型,并采取分批加入底物和添加树脂的方式解除底物和产物抑制.通过考察不同树脂对底物和产物的吸附量以及对生物还原反应的影响,筛选出了一种较适合的大孔吸附树脂NKA-Ⅱ.在优化的树脂加入量和加入模式下,当底物浓度为180 mmol/L时,产物产率由35.0%提高到71.2%,对映体过量值(ee)保持在95%左右.
이양주효모(Saccharomyces cerevisiae AS2.1392)전세포위최화제불대칭환원분갑선갑산갑지합성(R)-편도산갑지,해최화제최화속도쾌、조작은정성호.연구료저물화산물농도대반응초속도적영향,건립료저물화산물억제모형,병채취분비가입저물화첨가수지적방식해제저물화산물억제.통과고찰불동수지대저물화산물적흡부량이급대생물환원반응적영향,사선출료일충교괄합적대공흡부수지NKA-Ⅱ.재우화적수지가입량화가입모식하,당저물농도위180 mmol/L시,산물산솔유35.0%제고도71.2%,대영체과량치(ee)보지재95%좌우.
Whole cells of Saccharomyces cerevisiae AS2.1392 were used to catalyze the asymmetric reduction of methyl benzoylformate to form (R)-methyl mandelate.This biocatalytic process showed application potentials for its high catalytic activity and good operational stability.The inhibition effect of substrate and product on the bioreduction was evaluated by determining the initial reaction rate,and adsorbent resins were applied to alleviate the substrate and/or product inhibition.Due to the high adsorption capacity and the promoting effect on the bioreduction,resin NKA-Ⅱ was selected as the adsorbent material.Under the optimum resin amount and the proper adding mode,molecular yield of the product increased from 35.0% to 71.2% with an ee of (R)-methyl mandelate maintained 95% at the substrate concentration of 180 mmol/L.