国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2011年
3期
71-75
,共5页
李洁%张代涛%张新%黄芳%李锡太%贾蕾%刘园%严寒秋%黎新宇%王全意
李潔%張代濤%張新%黃芳%李錫太%賈蕾%劉園%嚴寒鞦%黎新宇%王全意
리길%장대도%장신%황방%리석태%가뢰%류완%엄한추%려신우%왕전의
病原学%急性出血性结膜炎%柯萨奇病毒A组24型变种
病原學%急性齣血性結膜炎%柯薩奇病毒A組24型變種
병원학%급성출혈성결막염%가살기병독A조24형변충
Etiology%Acute Hemorrhagic Conjunctivitis%CoxA24 variant
目的 了解2010年北京地区急性出血性结膜炎的病原学特征.方法 收集北京市2010年7月至10月疑似急性出血性结膜炎病例,采集患者眼拭子标本207件,采用荧光定量RT-PCR方法对标本进行CoxA24v、EV70病毒核酸检测,同时使用Hep-2细胞进行病毒分离.将获得的阳性CoxAE4v毒株进行VP1区基因全长的序列分析.结果 检测207例患者眼拭子标本,获得19例CoxA24v阳性样本,阳性率为9.2%.未检出EV70病毒核酸.使用Hep-2细胞进行病毒分离,获得7个CoxA24v毒株,将7个毒株的VP1区进行序列分析,结果显示该7株毒株组内核苷酸同源性为95.8%～99.7%.与2007年云南毒株、2008年浙江毒株核苷酸同源性分别为96%～99.5%和95.7%～100%,与2003年和2009年中国山东毒株核苷酸同源性分别为94.3%～94.6%和70.9%～73.4%.系统进化树分析结果显示该7株毒株位于3个独立的分支上.结论 北京地区2010年急性出血性结膜炎是由CoxA24v引起,北京地区存在至少3个CoxA24v流行株的传播.
目的 瞭解2010年北京地區急性齣血性結膜炎的病原學特徵.方法 收集北京市2010年7月至10月疑似急性齣血性結膜炎病例,採集患者眼拭子標本207件,採用熒光定量RT-PCR方法對標本進行CoxA24v、EV70病毒覈痠檢測,同時使用Hep-2細胞進行病毒分離.將穫得的暘性CoxAE4v毒株進行VP1區基因全長的序列分析.結果 檢測207例患者眼拭子標本,穫得19例CoxA24v暘性樣本,暘性率為9.2%.未檢齣EV70病毒覈痠.使用Hep-2細胞進行病毒分離,穫得7箇CoxA24v毒株,將7箇毒株的VP1區進行序列分析,結果顯示該7株毒株組內覈苷痠同源性為95.8%～99.7%.與2007年雲南毒株、2008年浙江毒株覈苷痠同源性分彆為96%～99.5%和95.7%～100%,與2003年和2009年中國山東毒株覈苷痠同源性分彆為94.3%～94.6%和70.9%～73.4%.繫統進化樹分析結果顯示該7株毒株位于3箇獨立的分支上.結論 北京地區2010年急性齣血性結膜炎是由CoxA24v引起,北京地區存在至少3箇CoxA24v流行株的傳播.
목적 료해2010년북경지구급성출혈성결막염적병원학특정.방법 수집북경시2010년7월지10월의사급성출혈성결막염병례,채집환자안식자표본207건,채용형광정량RT-PCR방법대표본진행CoxA24v、EV70병독핵산검측,동시사용Hep-2세포진행병독분리.장획득적양성CoxAE4v독주진행VP1구기인전장적서렬분석.결과 검측207례환자안식자표본,획득19례CoxA24v양성양본,양성솔위9.2%.미검출EV70병독핵산.사용Hep-2세포진행병독분리,획득7개CoxA24v독주,장7개독주적VP1구진행서렬분석,결과현시해7주독주조내핵감산동원성위95.8%～99.7%.여2007년운남독주、2008년절강독주핵감산동원성분별위96%～99.5%화95.7%～100%,여2003년화2009년중국산동독주핵감산동원성분별위94.3%～94.6%화70.9%～73.4%.계통진화수분석결과현시해7주독주위우3개독립적분지상.결론 북경지구2010년급성출혈성결막염시유CoxA24v인기,북경지구존재지소3개CoxA24v류행주적전파.
Objective To investigate the molecular characteristics of virus of acute hemorrhagic conjunctivitis detected in Beijing in 2010.Methods 207 conjunetival swab specimens of patients in Beijing during July and October were collected.Real-time RT-PCR method was used to type the specimens.At the same time,each specimen was,inoculated Hep-2 cells and cells were examined for the appearance of CPE.Positive cells that show CPE were detected by a real-time reverse transcription polymerase chain reaction (RT-PCR).PCR products were sequenced and analyzed.Results A total of 207 conjunctival swab specimens were detected and 19 positive CoxA24v specimens we got.No EV70 virus wag detectecd.7 positive CoxA24v PCR products were used to be sequenced.The 7 isolated strains have high homology of 95.8%～99.7%with each other.Compared with the nucleotide sequences of CoxA24v reference strain from Yunnan in 2007 and Zhejiang in 2008,the homology is 96%～99.5%and 95.7%～100%.Compared with the nucleotide sequences of CoxA24v reference strain from Shandong in 2003 and 2009,the homology is 94.3%～94.6%and 70.9%～73.4%.Phylogenetic analysis showed that the 7 isolates were located at 3 independent branch.Conclusion It was the CoxA24 variants that induced the epidemic of Acute Hemorrhagic Conjunctivitis in Beijing in 2010.And there were 3 or more individual epidemic virus strain in Beijing in 2010.
