中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2008年
5期
305-307
,共3页
朱庆云%李兆申%李淑德%胡先贵%满晓华%屠振兴
硃慶雲%李兆申%李淑德%鬍先貴%滿曉華%屠振興
주경운%리조신%리숙덕%호선귀%만효화%도진흥
胰腺肿瘤%RT-PCR%免疫组织化学%Fascin蛋白
胰腺腫瘤%RT-PCR%免疫組織化學%Fascin蛋白
이선종류%RT-PCR%면역조직화학%Fascin단백
Pancreatic neoplasms%Reverse transcription polymerase chain reaction%Immunohistochemistry%Fascin protein
目的 探讨运动相关蛋白Fascin在胰腺癌的表达及其与临床病理特征的关系.方法 采用RT-PCR法检测SW1990、Patu8988、BxPC3、CfPAC1 4株胰腺癌细胞株Fascin mRNA表达;采用免疫组化方法 检测54例胰腺癌及42例相应癌旁胰腺组织Fascin蛋白表达.结果 胰腺癌细胞株SW1990、Patu8988、CfPAC1有Fascin mRNA表达,BxPC3无表达;54例胰腺癌组织Fascin蛋白表达阳性35例,占64.8%,42例癌旁胰腺组织中均无阳性表达.Fascin蛋白表达与肿瘤分化程度(P<0.01)和淋巴转移(P<0.05)呈显著正相关,但与肿瘤大小及远处转移无相关性(P>0.05).结论 Fascin蛋白在胰腺癌组织中有较高的阳性表达率,检测其表达有助于胰腺癌的诊断,并有助于判断胰腺癌恶性程度.
目的 探討運動相關蛋白Fascin在胰腺癌的錶達及其與臨床病理特徵的關繫.方法 採用RT-PCR法檢測SW1990、Patu8988、BxPC3、CfPAC1 4株胰腺癌細胞株Fascin mRNA錶達;採用免疫組化方法 檢測54例胰腺癌及42例相應癌徬胰腺組織Fascin蛋白錶達.結果 胰腺癌細胞株SW1990、Patu8988、CfPAC1有Fascin mRNA錶達,BxPC3無錶達;54例胰腺癌組織Fascin蛋白錶達暘性35例,佔64.8%,42例癌徬胰腺組織中均無暘性錶達.Fascin蛋白錶達與腫瘤分化程度(P<0.01)和淋巴轉移(P<0.05)呈顯著正相關,但與腫瘤大小及遠處轉移無相關性(P>0.05).結論 Fascin蛋白在胰腺癌組織中有較高的暘性錶達率,檢測其錶達有助于胰腺癌的診斷,併有助于判斷胰腺癌噁性程度.
목적 탐토운동상관단백Fascin재이선암적표체급기여림상병리특정적관계.방법 채용RT-PCR법검측SW1990、Patu8988、BxPC3、CfPAC1 4주이선암세포주Fascin mRNA표체;채용면역조화방법 검측54례이선암급42례상응암방이선조직Fascin단백표체.결과 이선암세포주SW1990、Patu8988、CfPAC1유Fascin mRNA표체,BxPC3무표체;54례이선암조직Fascin단백표체양성35례,점64.8%,42례암방이선조직중균무양성표체.Fascin단백표체여종류분화정도(P<0.01)화림파전이(P<0.05)정현저정상관,단여종류대소급원처전이무상관성(P>0.05).결론 Fascin단백재이선암조직중유교고적양성표체솔,검측기표체유조우이선암적진단,병유조우판단이선암악성정도.
Objective To investigate the expression of Fascin mRNA and its protein in human pancreatic carcinoma cell line and pancreatic cancer tissues and to explore the relationship between the expression of Fascin protein and the clinicopathologic parameters. Methods The expression of Fascin mRNA in pancreatic cancer cell lines (SW1990, Patu8988, BxPC3, cfPAC1) were measured by RT-PCR and the expression of Fascin protein in 54 samples of pancreatic career tissues and 42 adjacent normal pancreatic tissues was detected by immunohistochemical method. Results The expression of Fascin mRNA was confirmed in 3 of 4 pancreatic cancer cell lines (SW1990, Patu8988, cfPAC1), but not in the cell line of BxPC3; the rate of positive expression of Fascin protein in pancreatic cancer tissues was 64.81% (35/54) and there was no positive expression in adjacent normal pancreas tissues; the expression of Fascin protein correlated with the differentiation degree (P < 0.01) and with the lymphatic metastasis of the carcinoma (P <0.05), but not with the size and distant metastasis of the carcinoma (P > 0. 05). Conclusions Fascin protein was highly positively expressed in pancreatic cancer tissues, and the expression of Fascin protein may help diagnose pancreatic, carcinoma and predict the malignant degree.
