CAJ | 학술논문

目的制备聚糖纳米微泡超声造影剂,观察其基本特征及动物超声显影效果.方法高速剪切法制备纳米微泡超声造影剂,光学显微镜和透射电镜观察纳米微泡大小、形态,马尔文粒度分析仪测粒径分布,细胞计数板计数纳米微泡,Zeta电位仪测表面电位,对比超声观察大鼠肾、心脏超声显影效果并测量声强度.结果纳米微泡呈空心球形结构,分散均匀,形态规则,平均粒径(617±12)nm,浓度(7.2±0.6)×109/ml,Zeta电位(52.9±1.3)mV.24 h、45 d和90d三个时间点浓度、平均粒径和表面电位无明显差异(P＞0.05).用其可使小鼠肾、心脏超声显影明显增强,最大视频强度分别达(15.6±1.1)GU、(27.3±2.5)GU,可视增强持续时间(10±2)min.结论聚糖纳米微泡稳定性和显像效果良好,有可能成为可穿过血管内皮间隙的新一代超声造影剂.
목적 제비취당납미미포초성조영제,관찰기기본특정급동물초성현영효과.방법 고속전절법제비납미미포초성조영제,광학현미경화투사전경관찰납미미포대소、형태,마이문립도분석의측립경분포,세포계수판계수납미미포,Zeta전위의측표면전위,대비초성관찰대서신、심장초성현영효과병측량성강도.결과 납미미포정공심구형결구,분산균균,형태규칙,평균립경(617±12)nm,농도(7.2±0.6)×109/ml,Zeta전위(52.9±1.3)mV.24 h、45 d화90d삼개시간점농도、평균립경화표면전위무명현차이(P＞0.05).용기가사소서신、심장초성현영명현증강,최대시빈강도분별체(15.6±1.1)GU、(27.3±2.5)GU,가시증강지속시간(10±2)min.결론 취당납미미포은정성화현상효과량호,유가능성위가천과혈관내피간극적신일대초성조영제.
Objective To develop nanometer-scale bubbles with surfaces of N-palmitoyl chitosan(PLCS) as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo. Methods The PLCS nanobubbles were prepared using a cutting technique at differential high-frequency of shear speed. Both optical and transmission electron micrography were performed to determine the nanobubble size and morphology. Concentration, size-distribution and zeta potential of the PLCS nanobubbles were measured by cell counting chamber, Malvern lazer particle analyzer and zeta-sizer at 1-day, 45-day and 90-day. The acoustic effects of the PLCS nanobubbles on myocardium and renal tissue in 6 normal rats were observed using bolus infusion of the nanobubbles intravenously. The maximum video intensity(VI) was measured.Results The PLCS nanobubbles with nice round-shape and uniform site-distribution were demonstrated.The mean diameter,concentration and zeta potential of the PLCS nanobubbles were (617 ± 12) nm, (7.2 ±0.6) × 109/ml and (52.9 ± 1.3)mV at the 1-day,and all of parameters did not change significantly in 45-day and 90-day ( P ＞ 0. 05). A significant contrast-enhancement was noted on myocardium and renal tissue during infusion of the nanobubbles. VI on both tissues was (15.6 ± 1.1)GU and (27.3 ± 2.5)GU,respectively. The visual contrast-enhancement last up to (10 ± 2)min. Conclusions The PLCS nanometerscale bubbles have excellent physical-features and contrast-enhanced ultrasound effects in vivo. It may develop as a novel contrast ultrasound agent which could cross endothelial cell membrances.