中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
2期
243-246
,共4页
李敏芝%田东莲%李敏%王艾红%李丽敏%郑龙%赵鹤龄
李敏芝%田東蓮%李敏%王艾紅%李麗敏%鄭龍%趙鶴齡
리민지%전동련%리민%왕애홍%리려민%정룡%조학령
洛伐他汀%脓毒症%一氧化氮合酶Ⅱ型%一氧化氮合酶Ⅲ型%主动脉,胸
洛伐他汀%膿毒癥%一氧化氮閤酶Ⅱ型%一氧化氮閤酶Ⅲ型%主動脈,胸
락벌타정%농독증%일양화담합매Ⅱ형%일양화담합매Ⅲ형%주동맥,흉
Lovastatin%Sepsis%Nitric oxide synthase type Ⅱ%Nitric oxide synthase type Ⅲ%Aorta,thoracic
目的 探讨辛伐他汀预处理对脓毒症大鼠胸主动脉诱导型一氧化氮合酶(iNOS)及内皮型一氧化氮合酶( eNOS)表达的影响.方法 清洁级雌性Wistar大鼠80只,4月龄,体重200～250 g,采用随机数字表法,将其随机分为4组:正常对照组(Ⅰ组,n=8)、假手术组(Ⅱ组,n=8)、脓毒症组(Ⅲ组,n=32)和辛伐他汀预处理组(Ⅳ组,n=32).Ⅱ组打开腹腔找到盲肠后还纳腹腔,缝合腹壁切口.Ⅲ组和Ⅳ组采用盲肠结扎穿孔法制备大鼠脓毒症模型.Ⅳ组制备脓毒症模型前经胃管灌注辛伐他汀20 mg/kg,1次/d,连续2周,Ⅰ组～Ⅲ组给予等容量生理盐水.Ⅱ组于假手术后6h处死动物,Ⅲ组和Ⅳ组于盲肠结扎穿孔后3、6、24、48 h分别取8只动物,处死,取胸主动脉,采用Western blot法测定iNOS和eNOS表达水平.结果 与Ⅰ组比较,Ⅱ组iNOS和eNOS表达差异无统计学意义(P＞0.05),Ⅲ组和Ⅳ组iNOS表达上调,eNOS表达下调(P＜0.05)；与Ⅲ组比较,Ⅳ组iNOS表达下调,eNOS表达上调(P＜0.05).结论 辛伐他汀预处理可下调脓毒症大鼠血管内皮细胞iNOS表达和上调血管内皮细胞eNOS表达,对血管内皮细胞功能有保护作用.
目的 探討辛伐他汀預處理對膿毒癥大鼠胸主動脈誘導型一氧化氮閤酶(iNOS)及內皮型一氧化氮閤酶( eNOS)錶達的影響.方法 清潔級雌性Wistar大鼠80隻,4月齡,體重200～250 g,採用隨機數字錶法,將其隨機分為4組:正常對照組(Ⅰ組,n=8)、假手術組(Ⅱ組,n=8)、膿毒癥組(Ⅲ組,n=32)和辛伐他汀預處理組(Ⅳ組,n=32).Ⅱ組打開腹腔找到盲腸後還納腹腔,縫閤腹壁切口.Ⅲ組和Ⅳ組採用盲腸結扎穿孔法製備大鼠膿毒癥模型.Ⅳ組製備膿毒癥模型前經胃管灌註辛伐他汀20 mg/kg,1次/d,連續2週,Ⅰ組～Ⅲ組給予等容量生理鹽水.Ⅱ組于假手術後6h處死動物,Ⅲ組和Ⅳ組于盲腸結扎穿孔後3、6、24、48 h分彆取8隻動物,處死,取胸主動脈,採用Western blot法測定iNOS和eNOS錶達水平.結果 與Ⅰ組比較,Ⅱ組iNOS和eNOS錶達差異無統計學意義(P＞0.05),Ⅲ組和Ⅳ組iNOS錶達上調,eNOS錶達下調(P＜0.05)；與Ⅲ組比較,Ⅳ組iNOS錶達下調,eNOS錶達上調(P＜0.05).結論 辛伐他汀預處理可下調膿毒癥大鼠血管內皮細胞iNOS錶達和上調血管內皮細胞eNOS錶達,對血管內皮細胞功能有保護作用.
목적 탐토신벌타정예처리대농독증대서흉주동맥유도형일양화담합매(iNOS)급내피형일양화담합매( eNOS)표체적영향.방법 청길급자성Wistar대서80지,4월령,체중200～250 g,채용수궤수자표법,장기수궤분위4조:정상대조조(Ⅰ조,n=8)、가수술조(Ⅱ조,n=8)、농독증조(Ⅲ조,n=32)화신벌타정예처리조(Ⅳ조,n=32).Ⅱ조타개복강조도맹장후환납복강,봉합복벽절구.Ⅲ조화Ⅳ조채용맹장결찰천공법제비대서농독증모형.Ⅳ조제비농독증모형전경위관관주신벌타정20 mg/kg,1차/d,련속2주,Ⅰ조～Ⅲ조급여등용량생리염수.Ⅱ조우가수술후6h처사동물,Ⅲ조화Ⅳ조우맹장결찰천공후3、6、24、48 h분별취8지동물,처사,취흉주동맥,채용Western blot법측정iNOS화eNOS표체수평.결과 여Ⅰ조비교,Ⅱ조iNOS화eNOS표체차이무통계학의의(P＞0.05),Ⅲ조화Ⅳ조iNOS표체상조,eNOS표체하조(P＜0.05)；여Ⅲ조비교,Ⅳ조iNOS표체하조,eNOS표체상조(P＜0.05).결론 신벌타정예처리가하조농독증대서혈관내피세포iNOS표체화상조혈관내피세포eNOS표체,대혈관내피세포공능유보호작용.
Objective To investigate the effects of simvastatin preconditioning on the expression of inducible and endothelial nitric oxide synthase ( iNOS,eNOS) in thoracic aorta in a rat model of sepsis.Methods Eighty pathogen-free female Wistar rats aged 4 months weighing 200-250 g were randomly divided into 4 groups:group normal control (group Ⅰ,n =8) ; group sham operation (group Ⅱ,n =8) ; group sepsis (group Ⅲ,n =32) and group simvastatin preconditioning (group Ⅳ,n =32).Sepsis was induced by cecal ligation and puncture (CLP) in groups Ⅲ and Ⅳ.In group Ⅳ simvastatin 20 mg/kg was given via a gastric tube once a day for 2 weeksbefore CLP.The thoracic aorta specimens were taken at 3,6,24 and 48 h after CLP (n =8 at each time point)for detection of iNOS and eNOS protein expression by Western blot analysis.Results CLP significantly up-regulated iNOS expression and down-regulated eNOS expression in group Ⅲ as compared with groups Ⅰ and Ⅱ.Simvastatin pretreatment significantly attenuated CLP-induced increase in iNOS expression and decrease in eNOS expression in group Ⅳ as compared with group Ⅲ.Conclusion Simvastatin preconditioning can protect vascular endothelial cells from septic injury by down-regulating iNOS expression and up-regulating eNOS expression in vascular endothelial cells.
