目的 观察碘对哺乳期大鼠甲状腺和乳腺钠碘转运体(NIS)、胰岛素样生长因子Ⅰ(IGF-Ⅰ)、转化生长因子β (TGF-β)mRNA表达的影响,探讨NIS、IGF-Ⅰ 、TGF-β mRNA在哺乳期大鼠甲状腺和乳腺摄碘中的作用.方法 选择Wistar大鼠101只,其中雌性80只,雄性21只,体质量80- 100g.按体质量将雌性大鼠随机分为5组:对照组(正常饲料、饮含碘50μg/L去离子水),低碘1组、2组(低碘饲料,分别饮用去离子水、含碘5 μg/L去离子水),高碘1组、2组(正常饲料,分别饮用含碘3000、10 000 μg/L去离子水),每组16只.在喂养3个月后,将雌鼠与雄鼠按3∶1合笼交配,在产后第5、10天,分别处死各组雌鼠,取甲状腺和乳腺,采用实时荧光定量PCR检测哺乳期大鼠甲状腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA表达.结果 产后第5天,哺乳期大鼠甲状腺和乳腺的NIS、IGF-Ⅰ 、TGF-β mRNA表达组间比较差异均有统计学意义(NIS:F值分别为631.46、64.91,P均＜0.01；IGF-Ⅰ:F值分别为11.45、6.56,P均＜0.01；TGF-β:F值分别为291.83、304.53,P均＜0.01).与对照组大鼠甲状腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0066±0.0023、(0.1481±0.0711)×10-2;IGF-Ⅰ:0.0419±0.0062、0.0542±0.0044;TGF:0.1416±0.0277、0.1670±0.0499]比较,低碘1组[NIS:0.0447±0.0110、(0.3030±0.1831)× 10-2;IGF-Ⅰ:0.0662±0.0078、0.0902±0.008;TGF-β:0.5514±0.0508、0.6942±0.0367]、2组[NIS:0.0317±0.0081 、(0.3017±0.1601)×10-2;IGF-Ⅰ:0.0645±0.0054、0.0894±0.0093;TGF-β:0.5292±0.0332、0.6704±0.0277]表达明显升高(P均＜0.01)；高碘1组NIS mRNA[0.0043±0.0011、(0.1233±0.0954)×10-2]、2组NIS mRNA[0.0037 ±0.0017、(0.1058±0.0854)×10-2]表达降低(P均＜0.05),但IGF-Ⅰ [0.0521±0.0910、0.0715±0.0026;0.0516±0.0078、0.0697±0.0038] 、TGF-β mRNA[0.2087±0.0425、0.2361±0.0425;0.1971±0.0237、0.2257±0.0752]表达升高(P均＜0.05).产后第10天,哺乳期大鼠甲状腺和乳腺的NIS、IGF-Ⅰ 、TGF-β mRNA表达组间比较差异均有统计学意义(NIS:F值分别为103.55、116.32,P均＜ 0.01;IGF-Ⅰ:F值分别为67.67、11.98,P均＜0.01；TGF-β:F值分别为74.30、381.30,P均＜0.01).与对照组大鼠甲状腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0069±0.0011 、(0.1337±0.0599)× 10-2;IGF-Ⅰ:0.0390±0.0071、0.0534±0.0056;TGF-β:0.1351±0.0336、0.1534±0.0320]比较,低碘1组[NIS:0.0432±0.0165、(0.2962±0.0985)× 10-2;IGF- Ⅰ:0.0643±0.0088、0.0873±0.0055;TGF-β:0.5042±0.0912、0.6408±0.0420]、2组[NIS:0.0287±0.0111、(0.2873±0.0862)×10-2;IGF-Ⅰ:0.0621±0.0094、0.0862±0.0038;TGF-β:0.4893±0.0504、0.6372±0.0389]表达明显升高(P均＜0.01)；高碘1组NIS mRNA [0.0042±0.0029、(0.1006±0.0909)× 10-2]、2组NIS mRNA [0.0035±0.0020、(0.0890±0.0119)×10-2]表达降低(P均＜0.01),但IGF-Ⅰ [0.0516±0.0078、0.0668±0.0071;0.0508±0.0089、0.0621±0.0064] 、TGF-β mRNA [0.2007±0.0546、0.2175±0.0370;0.1959±0.0393、0.2097±0.0425]表达升高(P均＜0.05).各组大鼠产后第5天与第10天比较,无论是甲状腺还是乳腺,NIS、IGF-Ⅰ 、TGF-β mRNA表达差异无统计学意义(P均＞0.05).结论 哺乳期大鼠甲状腺和乳腺对碘存在调控机制,低碘时甲状腺和乳腺NIS 、IGF-Ⅰ 、TGF-β mRNA表达增多,摄碘能力增强,满足机体的需要；高碘时甲状腺和乳腺NIS mRNA表达减少,由于摄碘能力降低,碘的摄入减少,减轻了高碘对仔鼠的危害.
目的 觀察碘對哺乳期大鼠甲狀腺和乳腺鈉碘轉運體(NIS)、胰島素樣生長因子Ⅰ(IGF-Ⅰ)、轉化生長因子β (TGF-β)mRNA錶達的影響,探討NIS、IGF-Ⅰ 、TGF-β mRNA在哺乳期大鼠甲狀腺和乳腺攝碘中的作用.方法 選擇Wistar大鼠101隻,其中雌性80隻,雄性21隻,體質量80- 100g.按體質量將雌性大鼠隨機分為5組:對照組(正常飼料、飲含碘50μg/L去離子水),低碘1組、2組(低碘飼料,分彆飲用去離子水、含碘5 μg/L去離子水),高碘1組、2組(正常飼料,分彆飲用含碘3000、10 000 μg/L去離子水),每組16隻.在餵養3箇月後,將雌鼠與雄鼠按3∶1閤籠交配,在產後第5、10天,分彆處死各組雌鼠,取甲狀腺和乳腺,採用實時熒光定量PCR檢測哺乳期大鼠甲狀腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA錶達.結果 產後第5天,哺乳期大鼠甲狀腺和乳腺的NIS、IGF-Ⅰ 、TGF-β mRNA錶達組間比較差異均有統計學意義(NIS:F值分彆為631.46、64.91,P均＜0.01；IGF-Ⅰ:F值分彆為11.45、6.56,P均＜0.01；TGF-β:F值分彆為291.83、304.53,P均＜0.01).與對照組大鼠甲狀腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0066±0.0023、(0.1481±0.0711)×10-2;IGF-Ⅰ:0.0419±0.0062、0.0542±0.0044;TGF:0.1416±0.0277、0.1670±0.0499]比較,低碘1組[NIS:0.0447±0.0110、(0.3030±0.1831)× 10-2;IGF-Ⅰ:0.0662±0.0078、0.0902±0.008;TGF-β:0.5514±0.0508、0.6942±0.0367]、2組[NIS:0.0317±0.0081 、(0.3017±0.1601)×10-2;IGF-Ⅰ:0.0645±0.0054、0.0894±0.0093;TGF-β:0.5292±0.0332、0.6704±0.0277]錶達明顯升高(P均＜0.01)；高碘1組NIS mRNA[0.0043±0.0011、(0.1233±0.0954)×10-2]、2組NIS mRNA[0.0037 ±0.0017、(0.1058±0.0854)×10-2]錶達降低(P均＜0.05),但IGF-Ⅰ [0.0521±0.0910、0.0715±0.0026;0.0516±0.0078、0.0697±0.0038] 、TGF-β mRNA[0.2087±0.0425、0.2361±0.0425;0.1971±0.0237、0.2257±0.0752]錶達升高(P均＜0.05).產後第10天,哺乳期大鼠甲狀腺和乳腺的NIS、IGF-Ⅰ 、TGF-β mRNA錶達組間比較差異均有統計學意義(NIS:F值分彆為103.55、116.32,P均＜ 0.01;IGF-Ⅰ:F值分彆為67.67、11.98,P均＜0.01；TGF-β:F值分彆為74.30、381.30,P均＜0.01).與對照組大鼠甲狀腺和乳腺NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0069±0.0011 、(0.1337±0.0599)× 10-2;IGF-Ⅰ:0.0390±0.0071、0.0534±0.0056;TGF-β:0.1351±0.0336、0.1534±0.0320]比較,低碘1組[NIS:0.0432±0.0165、(0.2962±0.0985)× 10-2;IGF- Ⅰ:0.0643±0.0088、0.0873±0.0055;TGF-β:0.5042±0.0912、0.6408±0.0420]、2組[NIS:0.0287±0.0111、(0.2873±0.0862)×10-2;IGF-Ⅰ:0.0621±0.0094、0.0862±0.0038;TGF-β:0.4893±0.0504、0.6372±0.0389]錶達明顯升高(P均＜0.01)；高碘1組NIS mRNA [0.0042±0.0029、(0.1006±0.0909)× 10-2]、2組NIS mRNA [0.0035±0.0020、(0.0890±0.0119)×10-2]錶達降低(P均＜0.01),但IGF-Ⅰ [0.0516±0.0078、0.0668±0.0071;0.0508±0.0089、0.0621±0.0064] 、TGF-β mRNA [0.2007±0.0546、0.2175±0.0370;0.1959±0.0393、0.2097±0.0425]錶達升高(P均＜0.05).各組大鼠產後第5天與第10天比較,無論是甲狀腺還是乳腺,NIS、IGF-Ⅰ 、TGF-β mRNA錶達差異無統計學意義(P均＞0.05).結論 哺乳期大鼠甲狀腺和乳腺對碘存在調控機製,低碘時甲狀腺和乳腺NIS 、IGF-Ⅰ 、TGF-β mRNA錶達增多,攝碘能力增彊,滿足機體的需要；高碘時甲狀腺和乳腺NIS mRNA錶達減少,由于攝碘能力降低,碘的攝入減少,減輕瞭高碘對仔鼠的危害.
목적 관찰전대포유기대서갑상선화유선납전전운체(NIS)、이도소양생장인자Ⅰ(IGF-Ⅰ)、전화생장인자β (TGF-β)mRNA표체적영향,탐토NIS、IGF-Ⅰ 、TGF-β mRNA재포유기대서갑상선화유선섭전중적작용.방법 선택Wistar대서101지,기중자성80지,웅성21지,체질량80- 100g.안체질량장자성대서수궤분위5조:대조조(정상사료、음함전50μg/L거리자수),저전1조、2조(저전사료,분별음용거리자수、함전5 μg/L거리자수),고전1조、2조(정상사료,분별음용함전3000、10 000 μg/L거리자수),매조16지.재위양3개월후,장자서여웅서안3∶1합롱교배,재산후제5、10천,분별처사각조자서,취갑상선화유선,채용실시형광정량PCR검측포유기대서갑상선화유선NIS、IGF-Ⅰ 、TGF-β mRNA표체.결과 산후제5천,포유기대서갑상선화유선적NIS、IGF-Ⅰ 、TGF-β mRNA표체조간비교차이균유통계학의의(NIS:F치분별위631.46、64.91,P균＜0.01；IGF-Ⅰ:F치분별위11.45、6.56,P균＜0.01；TGF-β:F치분별위291.83、304.53,P균＜0.01).여대조조대서갑상선화유선NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0066±0.0023、(0.1481±0.0711)×10-2;IGF-Ⅰ:0.0419±0.0062、0.0542±0.0044;TGF:0.1416±0.0277、0.1670±0.0499]비교,저전1조[NIS:0.0447±0.0110、(0.3030±0.1831)× 10-2;IGF-Ⅰ:0.0662±0.0078、0.0902±0.008;TGF-β:0.5514±0.0508、0.6942±0.0367]、2조[NIS:0.0317±0.0081 、(0.3017±0.1601)×10-2;IGF-Ⅰ:0.0645±0.0054、0.0894±0.0093;TGF-β:0.5292±0.0332、0.6704±0.0277]표체명현승고(P균＜0.01)；고전1조NIS mRNA[0.0043±0.0011、(0.1233±0.0954)×10-2]、2조NIS mRNA[0.0037 ±0.0017、(0.1058±0.0854)×10-2]표체강저(P균＜0.05),단IGF-Ⅰ [0.0521±0.0910、0.0715±0.0026;0.0516±0.0078、0.0697±0.0038] 、TGF-β mRNA[0.2087±0.0425、0.2361±0.0425;0.1971±0.0237、0.2257±0.0752]표체승고(P균＜0.05).산후제10천,포유기대서갑상선화유선적NIS、IGF-Ⅰ 、TGF-β mRNA표체조간비교차이균유통계학의의(NIS:F치분별위103.55、116.32,P균＜ 0.01;IGF-Ⅰ:F치분별위67.67、11.98,P균＜0.01；TGF-β:F치분별위74.30、381.30,P균＜0.01).여대조조대서갑상선화유선NIS、IGF-Ⅰ 、TGF-β mRNA[NIS:0.0069±0.0011 、(0.1337±0.0599)× 10-2;IGF-Ⅰ:0.0390±0.0071、0.0534±0.0056;TGF-β:0.1351±0.0336、0.1534±0.0320]비교,저전1조[NIS:0.0432±0.0165、(0.2962±0.0985)× 10-2;IGF- Ⅰ:0.0643±0.0088、0.0873±0.0055;TGF-β:0.5042±0.0912、0.6408±0.0420]、2조[NIS:0.0287±0.0111、(0.2873±0.0862)×10-2;IGF-Ⅰ:0.0621±0.0094、0.0862±0.0038;TGF-β:0.4893±0.0504、0.6372±0.0389]표체명현승고(P균＜0.01)；고전1조NIS mRNA [0.0042±0.0029、(0.1006±0.0909)× 10-2]、2조NIS mRNA [0.0035±0.0020、(0.0890±0.0119)×10-2]표체강저(P균＜0.01),단IGF-Ⅰ [0.0516±0.0078、0.0668±0.0071;0.0508±0.0089、0.0621±0.0064] 、TGF-β mRNA [0.2007±0.0546、0.2175±0.0370;0.1959±0.0393、0.2097±0.0425]표체승고(P균＜0.05).각조대서산후제5천여제10천비교,무론시갑상선환시유선,NIS、IGF-Ⅰ 、TGF-β mRNA표체차이무통계학의의(P균＞0.05).결론 포유기대서갑상선화유선대전존재조공궤제,저전시갑상선화유선NIS 、IGF-Ⅰ 、TGF-β mRNA표체증다,섭전능력증강,만족궤체적수요；고전시갑상선화유선NIS mRNA표체감소,유우섭전능력강저,전적섭입감소,감경료고전대자서적위해.
Objective To observe the influence of iodine on mRNA expression of iodide transporter (NIS),insulin-like growth factor Ⅰ (IGF- Ⅰ ) and transforming growth factor beta(TGF-β) in thyroid and mammary glands of lactating rats and to explore the role of NIS,IGF- Ⅰ and TGF-β mRNA in iodine uptake in thyroid and mammary glands of lactating rats.Methods One hundred and one Wistar rats(80 female and 21 male),weighting 8 - 100 g were selected.These female rats were randomly divided into five groups according to their body weight:control group(NI,normal feed,drank deionized water containing iodine 50 μg/L) ; low iodine group 1 and 2(LI-1,LI-2,low iodine feed,drank deionized water containing iodine 0 and 5 μg/L,respectively); high iodine group 1 and 2(HI-1,HI-2,normal feed,drank deionized water containing iodine 3000 and 10 000 μg/L,respectively),16 rats in each group.After feeding for 3 months,the female and male rates were mated 3:1.The female rats in each group were sacrificed at the fifth and tenth day after postpartum.Thyroid and mammary glands were taken.The mRNA levels of NIS,IGF- Ⅰ and TGF-β in thyroid and mammary glands of lactating rats were determined by real time quantitative PCR.Results The fifth days after postartum,NIS,IGF- Ⅰ and TGF-β mRNA expression levels of thyroid and lactating mammary glands were different between groups,and the differences were statistically significant ( NIS:F =631.46,64.91,all P ＜ 0.01 ; IGF- Ⅰ:F =11.45,6.56,all P ＜ 0.01 ; TGF-β:F =291.83,304.53,all P ＜ 0.01).Compared with control group [NIS:0.0066 ± 0.0023, (0.1481 ± 0.0711 ) × 10-2; IGF- Ⅰ:0.0419 ± 0.0062,0.0542 ± 0.0044; TGF-β:0.1416 ± 0.0277,0.1670 ± 0.0499],regardless of thyroid or mammary gland,the NIS,IGF- Ⅰ and TGF-β mRNA expression of LI-1 [NIS:0.0447 ± 0.0110,(0.3030 ± 0.1831) × 10-2;IGF- Ⅰ:0.0662 ± 0.0078,0.0902 ± 0.008; IGF- Ⅰ:0.5514 ± 0.0508,0.6942 ± 0.0367],LI-2[NIS:0.0317 ±0.0081,(0.3017 ± 0.1601) × 10-2; IGF-I:0.0645 ± 0.0054,0.0894 ± 0.0093; TGF-β:0.5292 ± 0.0332,0.6704 ± 0.0277 ] was significantly increased (all P ＜ 0.01 ); the NIS mRNA expression of HI-1 [0.0043 ± 0.0011,(0.1233 ± 0.0954) × 10-2],HI-2[0.0037 ± 0.0017,(0.1058 ± 0.0854) × 10-2] was decreased(all P ＜ 0.05),while the expression of IGF-Ⅰ mRNA [0.0521 ± 0.0910,0.0715 ± 0.0026; 0.0516 ± 0.0078,0.0697 ± 0.0038] and TGF-β mRNA [0.2087 ± 0.0425,0.2361 ± 0.0425; 0.1971 ± 0.0237,0.2257 ± 0.0752 ] was increased (all P ＜ 0.05 ).The tenth days after postpartum,the mRNA expression levels of NIS,IGF- Ⅰ and TGF-β of thyroid and lactating mammary gland in rats were different between groups,and the differences were statistically significant (NIS:F =103.55,116.32,all P ＜ 0.01; IGF-Ⅰ:F =67.67,11.98,all P ＜ 0.01; TGF-β:F =74.30,381.30,all P ＜0.01 ).Compared with the control group[NIS:0.0069 ± 0.0011,(0.1337 ± 0.0599) × 10-2; IGF-Ⅰ:0.0390 ±0.0071,0.0534 ± 0.0056; TGF-β:0.1351 ± 0.0336,0.1534 ± 0.0320],the mRNA expression levels of NIS,IGF- Ⅰ and TGF-β of LI-1 [ NIS:0.0432 ± 0.0165,(0.2962 ± 0.0985 ) × 10-2; IGF- Ⅰ:0.0643 ± 0.0088,0.0873 ± 0.0055 ; TGF-β:0.5042 ± 0.0912,0.6408 ± 0.0420],LI-2[NIS:0.0287 ± 0.0111,(0.2873 ± 0.0862) × 10-2; IGF- Ⅰ:0.0621 ± 0.0094,0.0862 ± 0.0038; TGF-β:0.4893 ± 0.0504,0.6372 ± 0.0389] were significantly increased(all P ＜ 0.01 ); the NIS mRNA levels of HI-1 [ 0.0042 ± 0.0029,(0.1006 ± 0.0909) × 10-2],HI-2[0.0035 ± 0.0020,(0.0890 ± 0.0119) × 10-2] were decreased(all P＜ 0.05),while the expression of IGF-Ⅰ mRNA[0.0516 ± 0.0078,0.0668 ± 0.0071; 0.0508 ± 0.0089,0.0621 ± 0.0064] and TGF-β mRNA[0.2007 ± 0.0546,0.2175 ± 0.0370;0.1959 ± 0.0393,0.2097 ± 0.0425] were increased(all P ＜ 0.05 ).In thyroid and mammary glands,the comparisons of NIS,IGF,TGF-β mRNA expression of the fifth and tenth day after postartum,between each group were not statistically significant(all P ＜ 0.05).Conclusions There are regulatory mechanisms of thyroid and mammary glands of lactating rats in response to low or high iodine conditions.In low iodine,the expressions of NIS,IGF- Ⅰ and TGF-β mRNA in thyroid and mammary glands increase and iodide uptake ability is enhanced to meet the body needs.In high iodine,the expression of NIS mRNA decreases in thyroid and mammary glands.Due to the reduced ability of iodine uptake,iodine intake is reduced,thereby reducing the hazards of high iodine in filial rats.
