CAJ | 학술논문

目的观察5-羟色胺1(5-HT1)受体激动剂对酸灌注脊髓损伤(SCI)猫膀胱排尿的影响.方法手术彻底离断雌猫脊髓12只,术后饲养2个月,氯醛糖麻醉猫后予膀胱置管,注入0.5％乙酸溶液同时行膀胱压力测定.静脉给与5-HTlA受体激动剂8-OH-DPAT(0.30 ～30.00μg/kg)或GR-46611 (0.03 ～300.00 μg/kg),最后给予5-HT1A受体拮抗剂WAY-100635(300.00 μg/kg),记录膀胱容量阈值、膀胱容量、残尿量、排尿量和血压,同时记录尿道外括约肌肌电图(EUS-EMG).结果 8-OH-DPAT作用酸灌注脊髓损伤猫后,其膀胱容量阈值、膀胱容量、残尿量等均呈剂量依赖性增加,剂量≥10μg/kg时反应明显,差异有统计学意义.而GR-46611无类似效应.WAY-100635能逆转8-OH-DPAT的大部分效应,但对GR-46611无影响.8-OH-DPAT和GR-46611对EUS-EMG均无作用.结论 5-HTlA受体激动剂可增加生理盐水灌注及酸灌注脊髓损伤猫的膀胱容量,提示以后可能作为临床上治疗脊髓损伤患者的药物,改善慢性脊髓损伤患者的膀胱过度活动及增加脊髓损伤患者的膀胱容量.
목적 관찰5-간색알1(5-HT1)수체격동제대산관주척수손상(SCI)묘방광배뇨적영향.방법 수술철저리단자묘척수12지,술후사양2개월,록철당마취묘후여방광치관,주입0.5％을산용액동시행방광압력측정.정맥급여5-HTlA수체격동제8-OH-DPAT(0.30 ～30.00μg/kg)혹GR-46611 (0.03 ～300.00 μg/kg),최후급여5-HT1A수체길항제WAY-100635(300.00 μg/kg),기록방광용량역치、방광용량、잔뇨량、배뇨량화혈압,동시기록뇨도외괄약기기전도(EUS-EMG).결과 8-OH-DPAT작용산관주척수손상묘후,기방광용량역치、방광용량、잔뇨량등균정제량의뢰성증가,제량≥10μg/kg시반응명현,차이유통계학의의.이GR-46611무유사효응.WAY-100635능역전8-OH-DPAT적대부분효응,단대GR-46611무영향.8-OH-DPAT화GR-46611대EUS-EMG균무작용.결론 5-HTlA수체격동제가증가생리염수관주급산관주척수손상묘적방광용량,제시이후가능작위림상상치료척수손상환자적약물,개선만성척수손상환자적방광과도활동급증가척수손상환자적방광용량.
Objective To evaluate the effects of 5-serotonin 1 (5-HT1) receptor agonists on acetic acid stimulated micturition in cats with chronic spinal cord injury (SCI).Methods Chloralose-anesthetized SCI cats were catheterized through the bladder dome for filling cystometry during 0.5％ acetic acid infusion.Dose-response curves for ( R)-8-OH-DPAT (0.30-30.00 μg/kg,i.v.) or GR 46611 (0.03-300.00 μg/kg,i.v.) were followed by 5-HT1A antagonist WAY-100635 (300.00 μg/kg).Threshold volume,bladder capacity,residual volume,micturition volume,and arterial pressure were measured and the external urethral sphincter electromyogram (EUS-EMG) was recorded.Results Acid-infused SCI cats responded to (R)-8-OH-DPAT but not GR-46611 with dose-dependent increases in threshold volume,capacity,and residual volume,significantly at the dose above 10 μg/kg.Effects of (R)-8-OH-DPAT were largely reversed by WAY 100635.Neither (R)-8-OH-DPAT nor GR-46611 augmented EUS-EMG activity.Conclusion Because 5-HT1A receptor agonists increase bladder capacity under either saline or acid infused conditions,they are promising candidates for reducing bladder hyperactivity and increasing bladder capacity in patients with chronic SCI.