CAJ | 학술논문

目的了解携带3种β内酰胺酶基因的鲍氏不动杆菌质粒的町传递性.方法选取从烧伤创面分离出的多重耐药鲍氏不动杆菌(供体菌),将之与大肠埃希菌ATCC 25922(受体菌)进行耐药质粒接合、药物敏感试验,并采用PCR分析接合子及其子代的耐药基因型、传代稳定性. 结果鲍氏不动杆菌通过接合将其携带对磺胺甲恶唑、氨苄西林、头孢噻吩、头孢博肟、头孢呋辛、亚胺培南/两司他丁和氨苄西林/舒巴坦的耐药性质粒及3种耐药基因传递给受体菌(例如经接合,使受体菌对磺胺甲恶唑的最低抑菌浓度>2 mg/L),且可稳定传代. 结论鲍氏不动杆菌质粒上携带可接合传递并稳定传代的β内酰胺酶基冈(blaTEM-1、blaPER-1、blaOXAS-23),是烧伤感染后其具有多重耐药性的分子生物学机制之一.
목적 료해휴대3충β내선알매기인적포씨불동간균질립적정전체성.방법 선취종소상창면분리출적다중내약포씨불동간균(공체균),장지여대장애희균ATCC 25922(수체균)진행내약질립접합、약물민감시험,병채용PCR분석접합자급기자대적내약기인형、전대은정성. 결과포씨불동간균통과접합장기휴대대광알갑악서、안변서림、두포새분、두포박우、두포부신、아알배남/량사타정화안변서림/서파탄적내약성질립급3충내약기인전체급수체균(례여경접합,사수체균대광알갑악서적최저억균농도>2 mg/L),차가은정전대. 결론포씨불동간균질립상휴대가접합전체병은정전대적β내선알매기강(blaTEM-1、blaPER-1、blaOXAS-23),시소상감염후기구유다중내약성적분자생물학궤제지일.
Objective To study the transferrable character of Aciuetobacter baumanni i(AB)plasraids with 3 types of beta-lactamase gene. Methods The plasmid of multi-drug resistant AB(donor)isolated from burn wound were transferred to E.coil ATCC25922 (receptor) through conjugation,and drug sensitivity was also observed.Drug-resistant gene and Stability of filial generation and zygote were analyzed by PCR. Results The dug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cetpodoxime,Cefuroxime,Imipenem/Cilastatin and Ampicillin/SuIbactam,and three types of beta-lactamase gene were transferred to the receptor,and were also stably transmitted for passages. The minimum inhibitor concentration ot receptor to Sulfamethoxazole was>2 mg/L after conjugation with donor,and inhibitory character could be transferred to next generation. Conclusion blaTEM-1,blaPER-1 and blaOXA-23 genes carried in the ptasmid of AB can be transferred through conjugation and stably transmitted for passages,and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.