CAJ | 학술논문

目的观察姜黄素对人食管癌EC-9706细胞增殖的影响并探讨其分子机制.方法应用50～200 μmol/L姜黄素处理食管癌EC-9706细胞株12～48 h后,应用四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制率、逆转录聚合酶链反应(RT-PCR)法检测热休克蛋白70(HSP70)mRNA的表达.结果经姜黄素干预后,人食管癌EC-9706 细胞生长减慢,MTT法检测结果显示增殖抑制率达18.3%～77.5%(P<0.05),呈良好的时间-剂量效应关系,HSP70 mRNA的表达下调.结论姜黄素能够有效抑制人食管癌细胞EC-9706的增殖,其机制可能与抑制HSP70 mRNA的表达有关.
목적 관찰강황소대인식관암EC-9706세포증식적영향병탐토기분자궤제.방법 응용50～200 μmol/L강황소처리식관암EC-9706세포주12～48 h후,응용사갑기우담서람(MTT)법검측세포증식억제솔、역전록취합매련반응(RT-PCR)법검측열휴극단백70(HSP70)mRNA적표체.결과 경강황소간예후,인식관암EC-9706 세포생장감만,MTT법검측결과현시증식억제솔체18.3%～77.5%(P<0.05),정량호적시간-제량효응관계,HSP70 mRNA적표체하조.결론 강황소능구유효억제인식관암세포EC-9706적증식,기궤제가능여억제HSP70 mRNA적표체유관.
Objective To explore the effect of curcumin on the proliferative inhibition of human esophageal cancer cell line EC-9706 and study the molecular mechanism. Methods After EC-9706 cells were treated with 50-200 μmol/L curcumin for 12-48 h,Methyl thiazolyl tetrazolium (MTT) method was used to detect the inhibition rates, and the expression of HSP70 mRNA was analyzed by RT-PCR. Results After curcumin treatment, the growth of EC-9706 cells was inhibited significantly in a time and dose-dependent manner, and the inhibition rates were 18.3%-77.5%(P<0.05).The expression of HSP70 mRNA was down-regulated (P<0.05). Conclusions Curcumin could effectively inhibit the proliferation of EC-9706 cells.Its mechanism may be inhibition of HSP70 mRNA expression.