中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
3期
12-14
,共3页
组织工程骨%骨缺损%筋膜瓣%血管化%成骨作用
組織工程骨%骨缺損%觔膜瓣%血管化%成骨作用
조직공정골%골결손%근막판%혈관화%성골작용
Tissue engineered bone%Bone defect%Fascia flap%Vascularization%Osteogenesis
目的 研究带蒂筋膜瓣包裹大段组织工程骨的长期成骨效果.方法 新西兰大白兔36只,每只兔双侧桡骨制作1.5 cm骨缺损,将组织工程骨植入骨缺损处,左侧用带蒂筋膜瓣将之包裹作为实验组,右侧不用带蒂筋膜瓣包裹作为对照组.术后早期2、4、6、8、12周行X线、ECT、组织学等手段检测;远期在术后6、12个月行X线及组织学检查,评价骨缺损修复情况.结果 术后早期标本的大体观察和组织学观察看,在各个时间段,实验组的骨痂生成和血管化成度均优于对照组,从X线片和ECT可以看出,术后2周实验组和对照组比较差异无统计学意义,以后的各个时间段,实验组的骨修复效果均明显优于对照组;远期X线和组织学显示组织工程骨与兔桡骨牢固愈合,并开始塑形且出现髓腔再通,β-TCP在体内逐渐被吸收,自身架构消失.结论 早期采用筋膜瓣包裹的方法能够显著提高组织工程骨修复大段骨缺损的能力,远期组织工程骨可以完全修复兔大段骨缺损,形成正常骨组织并发挥功能.
目的 研究帶蒂觔膜瓣包裹大段組織工程骨的長期成骨效果.方法 新西蘭大白兔36隻,每隻兔雙側橈骨製作1.5 cm骨缺損,將組織工程骨植入骨缺損處,左側用帶蒂觔膜瓣將之包裹作為實驗組,右側不用帶蒂觔膜瓣包裹作為對照組.術後早期2、4、6、8、12週行X線、ECT、組織學等手段檢測;遠期在術後6、12箇月行X線及組織學檢查,評價骨缺損脩複情況.結果 術後早期標本的大體觀察和組織學觀察看,在各箇時間段,實驗組的骨痂生成和血管化成度均優于對照組,從X線片和ECT可以看齣,術後2週實驗組和對照組比較差異無統計學意義,以後的各箇時間段,實驗組的骨脩複效果均明顯優于對照組;遠期X線和組織學顯示組織工程骨與兔橈骨牢固愈閤,併開始塑形且齣現髓腔再通,β-TCP在體內逐漸被吸收,自身架構消失.結論 早期採用觔膜瓣包裹的方法能夠顯著提高組織工程骨脩複大段骨缺損的能力,遠期組織工程骨可以完全脩複兔大段骨缺損,形成正常骨組織併髮揮功能.
목적 연구대체근막판포과대단조직공정골적장기성골효과.방법 신서란대백토36지,매지토쌍측뇨골제작1.5 cm골결손,장조직공정골식입골결손처,좌측용대체근막판장지포과작위실험조,우측불용대체근막판포과작위대조조.술후조기2、4、6、8、12주행X선、ECT、조직학등수단검측;원기재술후6、12개월행X선급조직학검사,평개골결손수복정황.결과 술후조기표본적대체관찰화조직학관찰간,재각개시간단,실험조적골가생성화혈관화성도균우우대조조,종X선편화ECT가이간출,술후2주실험조화대조조비교차이무통계학의의,이후적각개시간단,실험조적골수복효과균명현우우대조조;원기X선화조직학현시조직공정골여토뇨골뢰고유합,병개시소형차출현수강재통,β-TCP재체내축점피흡수,자신가구소실.결론 조기채용근막판포과적방법능구현저제고조직공정골수복대단골결손적능력,원기조직공정골가이완전수복토대단골결손,형성정상골조직병발휘공능.
Objective To investigate the long-term osteogenesis effects of large tissue engineered bone wraped up by blood supplied fascia. Methods Thirety -six New Zealand rabbits were subjected to operations to induce a 1.5 cm defect in rabbit' s two radiuses, which was filled subsequently with tissue engineered bone. The tissue engineering bone were wraped up by blood supplied fascia in rabbit's left radius, as the experimental group, the treatment wasn' t done to right radius, as the control group. At 2,4,6,8and 12 weeks after operation, X-ray examination, radionuclide bone scan and histological examination, were carried out to judge the osteogenesis effects. The repaired defects were evaluated by X-ray and histological examination at 6th , 12th month postoperation. Results In the early stage, gross observation and histology showed batter osteotylus generation and revascularization within 12 weeks postop-eratively experimental group than that in control group. Postoperative 2 weeks, X-ray and ECT displayed experiment group and control group do not have obvious difference, in each time later, the osteogenesis effects of the experiment group repairs was obviously superior to the control group. Longterm follow -up were performed by X -ray examination and histology at 6th, 12th month postoperatively revealed moulding of the new bones and medullary cavity recanalization, and the structure of β- TCP disappeared and gradually integrated into the new bones. Conclusions Early observation showed blood supplied fascia flap can accelerate the osteanagenesis process of tissue engineered bone. Long-term observation displayed tissue engineered bone is capable of total repair of large bone defect in rabbits by forming normal functional new bones.
