CAJ | 학술논문

以子叶节为外植体,建立起了紫穗槐的快速离体再生系统.经过四周的培养,在附加8mg·L-16-BA的MS培养基上能够获得再生频率为100%,平均每个外植体5.21个芽点的高效再生植株.以再生植株的茎节为外植体所进行的继代能够在相同的培养基上连续的产生新的不定芽,但芽点数要少于起始培养.经过3周的培养,有82.53%切下的再生茎段能够在含2.0mg·L-1IAA的MS培养基上生根.在所有进行分析过的再生植株中,它们的染色体数目都没有发生变异(2n=40).经过练苗以后,再生植株成功地定植于土壤当中并展示了一致的外部形态和生长特性.
이자협절위외식체,건립기료자수괴적쾌속리체재생계통.경과사주적배양,재부가8mg·L-16-BA적MS배양기상능구획득재생빈솔위100%,평균매개외식체5.21개아점적고효재생식주.이재생식주적경절위외식체소진행적계대능구재상동적배양기상련속적산생신적불정아,단아점수요소우기시배양.경과3주적배양,유82.53%절하적재생경단능구재함2.0mg·L-1IAA적MS배양기상생근.재소유진행분석과적재생식주중,타문적염색체수목도몰유발생변이(2n=40).경과련묘이후,재생식주성공지정식우토양당중병전시료일치적외부형태화생장특성.
Using cotyledonary nodes as explants,a rapid and efficient method for propagation of Amorpha fruticosa L.has been developed in the present paper.When grown on Murashige and Shoog medium (MS) supplemented with 8 mg·L-1 BAP for adventitious shoots induction,100% explants responded with 5.21 shoots per explant after 4 weeks culture.Explants taken from the in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same medium,but the shoot number per subculture was lower than that of the initiatial culture.82.53% excised shoots were rooted on half-strength MS medium containing 2.0 mg·L-1 IAA after 3 weeks of incubation.The regenerated plantlets possessed normal diploid chromosome number (2n=40) in all the analyzed cells.After acclimatization,all of the rooted plantlets established in soil,exhibiting uniform morphological and growth characteristics.