热带亚热带植物学报
熱帶亞熱帶植物學報
열대아열대식물학보
JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY
2001年
1期
69-74
,共6页
王昌虎%马镇荣%刘卫%吴坤林%张奕奇%凌定厚
王昌虎%馬鎮榮%劉衛%吳坤林%張奕奇%凌定厚
왕창호%마진영%류위%오곤림%장혁기%릉정후
正交设计%香蕉%直接出芽
正交設計%香蕉%直接齣芽
정교설계%향초%직접출아
利用正交设计方法[L27(313)]研究了培养基中的植物生长调节物BAP、NAA和IBA,以及不同外植体对香蕉(Musa acuminata Colla cv.Williams)直接出芽频率的影响。外植体的不同是影响香蕉直接出芽频率的最主要因素,NAA和IBA对实验结果的影响相同,均弱于BAP。实验结果的统计分析表明,诱导香蕉外植体直接出芽的优选培养基为不添加BAP、NAA和IBA的MS基本培养基,最佳外植体为芽的顶端分生组织部分。
利用正交設計方法[L27(313)]研究瞭培養基中的植物生長調節物BAP、NAA和IBA,以及不同外植體對香蕉(Musa acuminata Colla cv.Williams)直接齣芽頻率的影響。外植體的不同是影響香蕉直接齣芽頻率的最主要因素,NAA和IBA對實驗結果的影響相同,均弱于BAP。實驗結果的統計分析錶明,誘導香蕉外植體直接齣芽的優選培養基為不添加BAP、NAA和IBA的MS基本培養基,最佳外植體為芽的頂耑分生組織部分。
이용정교설계방법[L27(313)]연구료배양기중적식물생장조절물BAP、NAA화IBA,이급불동외식체대향초(Musa acuminata Colla cv.Williams)직접출아빈솔적영향。외식체적불동시영향향초직접출아빈솔적최주요인소,NAA화IBA대실험결과적영향상동,균약우BAP。실험결과적통계분석표명,유도향초외식체직접출아적우선배양기위불첨가BAP、NAA화IBA적MS기본배양기,최가외식체위아적정단분생조직부분。
Orthogonal experimental design was adopted to tissue culture in banana (Musa acuminata Colla cv. Williams) . Effects of BAP, NAA, IBA and different explants on explant direct budding were investigated in banana by using orthogonal design [L27 (313)]. Statistic analyses indicated that the main factor which mostly influenced the results was explants. BAP was the second factor, and both NAA and IBA the third. The results showed that the optimal medium was MS basal medium supplemented without the three growth regulators, and the best explant for the medium was the apical meristem of shoots.