CAJ | 학술논문

目的 :研究人心房肽 (hANP) cDNA转染细胞的微囊化技术中心房肽分泌的近日节律;通过人工调控,改变心房肽分泌的近日节律 ,达到有效治疗高血压或心力衰竭的目的. 方法 :将人心房肽基因( cDNA)转染入中国仓鼠卵巢细胞( Chinese hamster ovary,CHO),然后将细胞包被在聚己内酯管内形成微囊,并检测转基因 CHO细胞长时间存活情况和分泌的心房肽.检测微囊化转基因细胞分泌心房肽的生物节律,并用褪黑素进行调整. 结果 :在培养期间,长度 20 mm,直径 2 mm的聚己内酯管内转染 CHO细胞在 2 mL培养基中 24 h分泌的心房肽水平可达 210.3 ng/L;心房肽的分泌呈近日节律性变化,日间高,而夜间低;近日节律变化的时相是 4:15,用褪黑素处理,近日节律的时相移至 7:55. 结论 :心房肽 cDNA转染的 CHO细胞包被在聚己内酯管内并能向管外分泌心房肽,将来可能适于植入人体.此项研究为心房肽治疗高血压或心力衰竭提供了一条新途径.
목적 :연구인심방태 (hANP) cDNA전염세포적미낭화기술중심방태분비적근일절률;통과인공조공,개변심방태분비적근일절률 ,체도유효치료고혈압혹심력쇠갈적목적. 방법 :장인심방태기인( cDNA)전염입중국창서란소세포( Chinese hamster ovary,CHO),연후장세포포피재취기내지관내형성미낭,병검측전기인 CHO세포장시간존활정황화분비적심방태.검측미낭화전기인세포분비심방태적생물절률,병용퇴흑소진행조정. 결과 :재배양기간,장도 20 mm,직경 2 mm적취기내지관내전염 CHO세포재 2 mL배양기중 24 h분비적심방태수평가체 210.3 ng/L;심방태적분비정근일절률성변화,일간고,이야간저;근일절률변화적시상시 4:15,용퇴흑소처리,근일절률적시상이지 7:55. 결론 :심방태 cDNA전염적 CHO세포포피재취기내지관내병능향관외분비심방태,장래가능괄우식입인체.차항연구위심방태치료고혈압혹심력쇠갈제공료일조신도경.
AIM:To investigate atrial natriuretic peptide(ANP) circadian in the encapsulated human ANP(hANP) cDNA transfected cells,to alter the ANP circadian by artificial control to achieve the objective of effectively treat hypertension or congestive heart failure(CHF). METHODS:ANP cDNA was transfected into Chinese hamster ovary(CHO) cells,which were encapsulated in polycarprolactone(PCL) tubes.The longterm survival of transfected CHO cells and the levels of ANP secreted were detected.Circadian rhythm of ANP secreted by encapsulated transfected cells was also studied,which was regulated by melatonin. RESULTS:During culturing,the ANP level secreted by transfected CHO cells in 2 mL of culture medium within 24 hours could reach 210.3 ng/L in a 20 mm-long and 2 mm-diameter PCL tube.The section of ANP displayed a circadian variation:higher in daytime,but lower at night.The acrophase of circadian rhythm was 4:15 but could be shifted to 7:55 after melatonin management. CONCLUSION:ANP cDNA transfected CHO cells that encapsulated into PCL tube can secret ANP,which might be suitable for the future implantation into human body.Our research provides a new approach in the treatment of hypertension and CHF by ANP.