肿瘤
腫瘤
종류
TUMOR
2009年
2期
131-134
,共4页
肺肿瘤%RhoC%RhoGDI%转移
肺腫瘤%RhoC%RhoGDI%轉移
폐종류%RhoC%RhoGDI%전이
目的:研究ras同系物(ras homologue,Rho)C蛋白及其调节蛋白Rho二磷酸鸟苷解离抑制因子(Rho guanosine diphosphate dissociation inhibitor, RhoGDI)-β和-γ在肺癌细胞中的表达情况.方法:应用Western印迹法和RT-PCR检测人正常支气管上皮细胞和不同人肺癌细胞系中RhoC、RhoGDIβ和RhoGDIγ蛋白及mRNA的表达.结果:RhoC、RhoGDIβ和RhoGDIγ的蛋白及mRNA在人肺腺癌细胞系、人肺巨细胞癌细胞系(高和低侵袭力亚型)和人支气管上皮细胞系中均有表达.3种肺癌细胞系中RhoC、RhoGDIβ和RhoGDIγ的表达均明显高于人支气管上皮细胞系.高侵袭力人肺巨细胞癌细胞系BE1中RhoC和RhoGDIγ的表达均明显高于低侵袭力人肺巨细胞癌细胞系LH7.低侵袭力人肺巨细胞癌细胞系LH7中RhoGDIβ的表达高于高侵袭力人肺巨细胞癌细胞系BE1.结论:高侵袭力人肺巨细胞癌细胞BE1中RhoC和RhoGDIγ的表达较高,低侵袭力人肺巨细胞癌细胞系LH7中RhoGDIβ的表达较高.
目的:研究ras同繫物(ras homologue,Rho)C蛋白及其調節蛋白Rho二燐痠鳥苷解離抑製因子(Rho guanosine diphosphate dissociation inhibitor, RhoGDI)-β和-γ在肺癌細胞中的錶達情況.方法:應用Western印跡法和RT-PCR檢測人正常支氣管上皮細胞和不同人肺癌細胞繫中RhoC、RhoGDIβ和RhoGDIγ蛋白及mRNA的錶達.結果:RhoC、RhoGDIβ和RhoGDIγ的蛋白及mRNA在人肺腺癌細胞繫、人肺巨細胞癌細胞繫(高和低侵襲力亞型)和人支氣管上皮細胞繫中均有錶達.3種肺癌細胞繫中RhoC、RhoGDIβ和RhoGDIγ的錶達均明顯高于人支氣管上皮細胞繫.高侵襲力人肺巨細胞癌細胞繫BE1中RhoC和RhoGDIγ的錶達均明顯高于低侵襲力人肺巨細胞癌細胞繫LH7.低侵襲力人肺巨細胞癌細胞繫LH7中RhoGDIβ的錶達高于高侵襲力人肺巨細胞癌細胞繫BE1.結論:高侵襲力人肺巨細胞癌細胞BE1中RhoC和RhoGDIγ的錶達較高,低侵襲力人肺巨細胞癌細胞繫LH7中RhoGDIβ的錶達較高.
목적:연구ras동계물(ras homologue,Rho)C단백급기조절단백Rho이린산조감해리억제인자(Rho guanosine diphosphate dissociation inhibitor, RhoGDI)-β화-γ재폐암세포중적표체정황.방법:응용Western인적법화RT-PCR검측인정상지기관상피세포화불동인폐암세포계중RhoC、RhoGDIβ화RhoGDIγ단백급mRNA적표체.결과:RhoC、RhoGDIβ화RhoGDIγ적단백급mRNA재인폐선암세포계、인폐거세포암세포계(고화저침습력아형)화인지기관상피세포계중균유표체.3충폐암세포계중RhoC、RhoGDIβ화RhoGDIγ적표체균명현고우인지기관상피세포계.고침습력인폐거세포암세포계BE1중RhoC화RhoGDIγ적표체균명현고우저침습력인폐거세포암세포계LH7.저침습력인폐거세포암세포계LH7중RhoGDIβ적표체고우고침습력인폐거세포암세포계BE1.결론:고침습력인폐거세포암세포BE1중RhoC화RhoGDIγ적표체교고,저침습력인폐거세포암세포계LH7중RhoGDIβ적표체교고.
