国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2011年
3期
113-122
,共10页
冯洁%相蕾%万钢%齐科研%孙亮%史晓红%郑陈光%吕泽平%胡才有%杨泽
馮潔%相蕾%萬鋼%齊科研%孫亮%史曉紅%鄭陳光%呂澤平%鬍纔有%楊澤
풍길%상뢰%만강%제과연%손량%사효홍%정진광%려택평%호재유%양택
ApoE基因%健康长寿%长寿相关表型%Meta分析
ApoE基因%健康長壽%長壽相關錶型%Meta分析
ApoE기인%건강장수%장수상관표형%Meta분석
ApoE gene%Healthy longevity%Longevity-related phenotype%Meta-analysis
目的 该研究在多群体中筛查验证ApoE基因多态性与长寿的关联,并调查ApoE基因变异与长寿相关表型的关联.方法 该研究采用群体1筛查,群体2、3验证的策略,共收集814个长寿老人(年龄≥90岁)和1136个无长寿家族史、当地一般人群的对照组(年龄30～65岁).通过PCR-RFLP和测序方法对ApoE基因进行基因分型.结果 在3个群体中长寿老人的ApoE ε3/ε3频率明显高于对照人群,与长寿存在正关联,这个结果与以往其他群体的研究结果并不相同;而长寿老人中ApoE ε3/ε4 频率明显低于对照群体,与长寿存在负关联.同时,在长寿老人中,ApoE ε3/ε3携带者比未携带ApoEε3/ε3者的高密度脂蛋白水平高(F=6.970,P=0.005),ApoE ε4携带者(ε3/ε4和ε4/ε4基因型总和)比非ApoEε4携带者的总胆固醇和低密度脂蛋白水平高(F=4.810,P=0.003和F=4.21 8,P=0.012).另外,该文对世界不同群体进行Meta分析,结果显示ApoE ε4携带者与长寿负关联(合并OR=0.42,95%CI:0.36～0.49),是长寿的风险因素.ε3/ε3基因型与长寿正关联(合并 OR=1.47,95%CI:1.25～1.74),是长寿的保护因素.结论 多个群体验证ApoE ε3/ε3可能是长寿的保护因素,这与以往报道ApoE ε2/ε2是长寿保护因素的结果不同;而ApoEε4是长寿的风险因素.
目的 該研究在多群體中篩查驗證ApoE基因多態性與長壽的關聯,併調查ApoE基因變異與長壽相關錶型的關聯.方法 該研究採用群體1篩查,群體2、3驗證的策略,共收集814箇長壽老人(年齡≥90歲)和1136箇無長壽傢族史、噹地一般人群的對照組(年齡30～65歲).通過PCR-RFLP和測序方法對ApoE基因進行基因分型.結果 在3箇群體中長壽老人的ApoE ε3/ε3頻率明顯高于對照人群,與長壽存在正關聯,這箇結果與以往其他群體的研究結果併不相同;而長壽老人中ApoE ε3/ε4 頻率明顯低于對照群體,與長壽存在負關聯.同時,在長壽老人中,ApoE ε3/ε3攜帶者比未攜帶ApoEε3/ε3者的高密度脂蛋白水平高(F=6.970,P=0.005),ApoE ε4攜帶者(ε3/ε4和ε4/ε4基因型總和)比非ApoEε4攜帶者的總膽固醇和低密度脂蛋白水平高(F=4.810,P=0.003和F=4.21 8,P=0.012).另外,該文對世界不同群體進行Meta分析,結果顯示ApoE ε4攜帶者與長壽負關聯(閤併OR=0.42,95%CI:0.36～0.49),是長壽的風險因素.ε3/ε3基因型與長壽正關聯(閤併 OR=1.47,95%CI:1.25～1.74),是長壽的保護因素.結論 多箇群體驗證ApoE ε3/ε3可能是長壽的保護因素,這與以往報道ApoE ε2/ε2是長壽保護因素的結果不同;而ApoEε4是長壽的風險因素.
목적 해연구재다군체중사사험증ApoE기인다태성여장수적관련,병조사ApoE기인변이여장수상관표형적관련.방법 해연구채용군체1사사,군체2、3험증적책략,공수집814개장수노인(년령≥90세)화1136개무장수가족사、당지일반인군적대조조(년령30～65세).통과PCR-RFLP화측서방법대ApoE기인진행기인분형.결과 재3개군체중장수노인적ApoE ε3/ε3빈솔명현고우대조인군,여장수존재정관련,저개결과여이왕기타군체적연구결과병불상동;이장수노인중ApoE ε3/ε4 빈솔명현저우대조군체,여장수존재부관련.동시,재장수노인중,ApoE ε3/ε3휴대자비미휴대ApoEε3/ε3자적고밀도지단백수평고(F=6.970,P=0.005),ApoE ε4휴대자(ε3/ε4화ε4/ε4기인형총화)비비ApoEε4휴대자적총담고순화저밀도지단백수평고(F=4.810,P=0.003화F=4.21 8,P=0.012).령외,해문대세계불동군체진행Meta분석,결과현시ApoE ε4휴대자여장수부관련(합병OR=0.42,95%CI:0.36～0.49),시장수적풍험인소.ε3/ε3기인형여장수정관련(합병 OR=1.47,95%CI:1.25～1.74),시장수적보호인소.결론 다개군체험증ApoE ε3/ε3가능시장수적보호인소,저여이왕보도ApoE ε2/ε2시장수보호인소적결과불동;이ApoEε4시장수적풍험인소.
Objective The present study is to identify and replicate the association of ApoE longevity in multi-populations, and observe the association of ApoE with longevity-related phenotype. Methods By multi-population design:identifying ApoE gene associated with longevity in population 1, and replicating ApoE gene associated with longevity in population 2 and 3. A total of 814 "longevity cases" were classified as participants who had survived to age 90 years or more, with a total of 1136" younger controls" less than 65 years of age. ApoE gene was genotyped by PCR-RFLP and sequencing. Results Results showed the homozygous ε3/ε3 genotype in long-lived population was significantly higher frequent than those in controls,positively associated with longevity, whereas the heterozygous ε3/ε4 genotype were significantly lower frequent than those in controls, negatively associated with longevity in 3 different populations. ApoE ε3/ε3 was associated with higher HDL-C levels(F=6.970,P=0.005),and ApoE ε4-carrier(the ε3/ε4 and ε4/ε4 genotypes) was associated with significantly higher TC and LDL-C levels(F=4.810,P =0.003 and F=4.218,P=0.012)in long-lived individuals.In addition, the meta-analysis in 14 populations in world suggested ε4-carriers were negatively associated with longevity(pool ORs=0. 42,95% CI:0. 36～0.49);whereas the ApoE ε3/ε3 genotype was positively associated (pool ORs=1.47,95% CI:1.25~1.74) with longevity. Conclusions It was confirmed ApoE gene was associated with longevity in multi-population. ApoE ε3 gene could conferred protective effect for healthy longevity which was inconsistent the previous results published , whereas ApoE ε4 gene increased the risk effect for successful aging and longevity.
