中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
7期
472-475
,共4页
徐振兴%陈进春%邱明山%滕菁%徐明
徐振興%陳進春%邱明山%滕菁%徐明
서진흥%진진춘%구명산%등정%서명
关节炎,类风湿%多态性,单核苷酸%蛋白质酪氨酸磷酸酶%聚合酶链反应
關節炎,類風濕%多態性,單覈苷痠%蛋白質酪氨痠燐痠酶%聚閤酶鏈反應
관절염,류풍습%다태성,단핵감산%단백질락안산린산매%취합매련반응
Arthritis,rheumatoid%Polymorphism,single nucleotide%Protein-tyrosine-phosphatase%Polymerase chain reaction
目的 建立TaqMan-MGB探针实时荧光聚合酶链反应(PCR)方法,研究福建厦门地区汉族人类风湿关节炎(RA)与PTPN22基因C 1858T多态性的相关性.方法 应用病例对照法,收集100例RA患者和100名健康人血液标本,利用TaqMan-MGB探针实时荧光PCR方法检测PTPN22C1858T基因多态性.结果 ①成功建立TaqMan-MGB探针实时荧光PCR技术检测PTPN22C1858T基因多态性.②PTPN22基因1858位点在福建厦门地区汉族人中仅存在C等位基因,未检测到T等位基因.结论 厦门地区汉族人不存在PTPN22 1858T等位基因,PTPN22 C1858T多态性与RA可能不存在关联.
目的 建立TaqMan-MGB探針實時熒光聚閤酶鏈反應(PCR)方法,研究福建廈門地區漢族人類風濕關節炎(RA)與PTPN22基因C 1858T多態性的相關性.方法 應用病例對照法,收集100例RA患者和100名健康人血液標本,利用TaqMan-MGB探針實時熒光PCR方法檢測PTPN22C1858T基因多態性.結果 ①成功建立TaqMan-MGB探針實時熒光PCR技術檢測PTPN22C1858T基因多態性.②PTPN22基因1858位點在福建廈門地區漢族人中僅存在C等位基因,未檢測到T等位基因.結論 廈門地區漢族人不存在PTPN22 1858T等位基因,PTPN22 C1858T多態性與RA可能不存在關聯.
목적 건립TaqMan-MGB탐침실시형광취합매련반응(PCR)방법,연구복건하문지구한족인류풍습관절염(RA)여PTPN22기인C 1858T다태성적상관성.방법 응용병례대조법,수집100례RA환자화100명건강인혈액표본,이용TaqMan-MGB탐침실시형광PCR방법검측PTPN22C1858T기인다태성.결과 ①성공건립TaqMan-MGB탐침실시형광PCR기술검측PTPN22C1858T기인다태성.②PTPN22기인1858위점재복건하문지구한족인중부존재C등위기인,미검측도T등위기인.결론 하문지구한족인불존재PTPN22 1858T등위기인,PTPN22 C1858T다태성여RA가능불존재관련.
Objective To assess the correlation between FFPN22 C1858T polymorphism and rheumatoid arthritis(RA)in Han people in Xiamen area by TaqMan-MGB real-time PCR．Methods A casecontrol study was carried out in Xiamen Han population．Their blood samples(100 RAs and 100 controls respectively)were collected and the PTPN22 C1858T polymnrphism was tested by TaqMan-MGB real-time PCR．Results ①A technique of TaqMan-MGB teal-time PCR was established to investigate PTPN22 C1858T polymorphism；② Onlv 1858 C allele was presented in all the RAs and controls，and no T allele was detected．Conclusion There is no PTPN22 1858T allele in Han people in Xiamen area．It is suggested that there's no association between PTPN22 C1858T polymorphism and RA．
