中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
5期
320-322
,共3页
周国雄%张淼%丁晓凌%张海峰%曹维%瞿利帅%蒯小玲%张弘%徐正府
週國雄%張淼%丁曉凌%張海峰%曹維%瞿利帥%蒯小玲%張弘%徐正府
주국웅%장묘%정효릉%장해봉%조유%구리수%괴소령%장홍%서정부
胰腺肿瘤%半乳糖凝集素3%细胞增殖%肿瘤浸润
胰腺腫瘤%半乳糖凝集素3%細胞增殖%腫瘤浸潤
이선종류%반유당응집소3%세포증식%종류침윤
Pancreatic neoplasms%Galectin-3%Cell proliferation%Neoplasm invasiveness
目的 研究半乳凝素3( galectin-3)在胰腺癌细胞株中的表达及对人胰腺癌细胞株SW1990增殖和侵袭能力的影响.方法 采用免疫细胞化学法和RT-PCR方法检测胰腺癌细胞株SW1990、PANC1、AsPC-1的galectin-3蛋白和mRNA表达.分别应用1、2、3、5μg/ml galectin-3单抗处理SW1990细胞24、48、72 h,采用CCK-8试剂盒检测细胞的增殖,Transwell小室检测细胞的侵袭能力.结果 胰腺癌SW1990.PANC1、AsPC-1细胞均有galectin-3 mRNA和蛋白表达.galectin-3单抗呈浓度和时间依赖性抑制SW1990细胞的增殖及穿膜细胞数.培养72 h时2、3、5μg/ml galectin-3单抗的细胞生长抑制率分别为19.8％、29.9％和42.7％,与对照组比较差异均有统计学意义(P值均＜0.05).3 μg/ml galectin-3单抗组的穿膜细胞抑制率为37.1％,与对照组的10.4％差异有统计学意义(P＜0.05).结论 galectin-3在胰腺癌细胞中高表达,用抗体中和galectin-3能抑制SW1990细胞的增殖和侵袭能力
目的 研究半乳凝素3( galectin-3)在胰腺癌細胞株中的錶達及對人胰腺癌細胞株SW1990增殖和侵襲能力的影響.方法 採用免疫細胞化學法和RT-PCR方法檢測胰腺癌細胞株SW1990、PANC1、AsPC-1的galectin-3蛋白和mRNA錶達.分彆應用1、2、3、5μg/ml galectin-3單抗處理SW1990細胞24、48、72 h,採用CCK-8試劑盒檢測細胞的增殖,Transwell小室檢測細胞的侵襲能力.結果 胰腺癌SW1990.PANC1、AsPC-1細胞均有galectin-3 mRNA和蛋白錶達.galectin-3單抗呈濃度和時間依賴性抑製SW1990細胞的增殖及穿膜細胞數.培養72 h時2、3、5μg/ml galectin-3單抗的細胞生長抑製率分彆為19.8％、29.9％和42.7％,與對照組比較差異均有統計學意義(P值均＜0.05).3 μg/ml galectin-3單抗組的穿膜細胞抑製率為37.1％,與對照組的10.4％差異有統計學意義(P＜0.05).結論 galectin-3在胰腺癌細胞中高錶達,用抗體中和galectin-3能抑製SW1990細胞的增殖和侵襲能力
목적 연구반유응소3( galectin-3)재이선암세포주중적표체급대인이선암세포주SW1990증식화침습능력적영향.방법 채용면역세포화학법화RT-PCR방법검측이선암세포주SW1990、PANC1、AsPC-1적galectin-3단백화mRNA표체.분별응용1、2、3、5μg/ml galectin-3단항처리SW1990세포24、48、72 h,채용CCK-8시제합검측세포적증식,Transwell소실검측세포적침습능력.결과 이선암SW1990.PANC1、AsPC-1세포균유galectin-3 mRNA화단백표체.galectin-3단항정농도화시간의뢰성억제SW1990세포적증식급천막세포수.배양72 h시2、3、5μg/ml galectin-3단항적세포생장억제솔분별위19.8％、29.9％화42.7％,여대조조비교차이균유통계학의의(P치균＜0.05).3 μg/ml galectin-3단항조적천막세포억제솔위37.1％,여대조조적10.4％차이유통계학의의(P＜0.05).결론 galectin-3재이선암세포중고표체,용항체중화galectin-3능억제SW1990세포적증식화침습능력
Objective To investigate the expression of galectin-3 in pancreatic cancer cell and its effect on the proliferation and invasion of SW1990.Methods Immunocytochemistry and semi-quantitative RT-PCR was used to detect the expression of galectin-3 protein and mRNA in SW1990,PANC1 and ASPC-1 cell lines.Galectin-3 mono-antibody of different concentrations ( 1,2,3,5 μg/ml) was used to treat SW1990 cells for 24,48,72 h,CCK-8 kits were used to detect the proliferation in SW1990 cells; Transwell chamber was used to study the invasion in SW1990.Results Expression of galectin-3 protein and mRNA was present in SW1990,PANC1,and ASPC-1.Galectin 3 mono-antibody inhibited the proliferation and number of invasive cells in a dose and time dependant manner.The inhibitory rates at 72 h were 19.8％,29.9％ and 42.7％ in 2,3,5 μg/ml galectin 3 mono-antibody groups,the difference among them and control group was statistically significant ( P ＜ 0.05 ).The inhibite rate of permeating membrane cells in 3 μg/ml galectin-3 mono antibody was 37.1％,the difference between this group and control group was statistically significant (P ＜0.05 ).ConcLusions Galectin-3 is highly expressed in pancreatic cancer cells.Galectin-3 antibody can inhibit the proliferation and migration capability of SW1990 cells.
