CAJ | 학술논문

背景人类和哺乳动物视觉发育关键期内视网膜处于发育阶段,其各个亚层的正确排列以及细胞的准确定位是视觉信息正常传递的基础之一.频域光学相关断层扫描(SD-OCT)可活体测量并连续观察大鼠视网膜厚度的变化.目的了解视觉发育关键期内SD大鼠视网膜厚度的变化规律.方法选择新生SD大鼠30只,其中10只于出生后14、18、21、24、42 d进行双眼眼底SD-OCT扫描,对视网膜厚度以及部分亚层包括内界膜(ILM)至内丛状层(IPL)、内核层(INL)、外核层(ONL)至视网膜色素上皮(RPE)层的厚度进行生物测量.另取20只SD大鼠,分别于14、18、21、24、42 d各处死4只大鼠并摘除双眼,制作石蜡切片行苏木精-伊红染色,对视网膜厚度以及部分亚层结构进行测量.并对两种方法测量数据的相关性进行评价.结果大鼠视网膜Cirrus HD-OCT图像由内向外可显示视网膜IPL、INL、外丛状层(OPL)、ONL、光感受器内节/外节(IS/OS)层以及RPE层等结构.比较各个时间点的视网膜厚度、ILM至IPL厚度、INL厚度以及ONL至RPE层厚度,差异均有统计学意义(F=15.425,P=0.000;F=3.973,P=0.007;F=17.529,P=0.000;F=7.038,P=0.000).随大鼠日龄的增加,视网膜厚度、INL厚度及ONL至RPE层厚度均呈递减趋势.测量视网膜组织切片各对应层次厚度,并与OCT测量结果进行线性回归分析,结果表明两种方法测量的视网膜厚度、INL厚度以及ONL至RPE层厚度均呈正相关(r=0.794,P=0.000;r=0.784,P=0.000;r=0.681,P=0.000).结论 Cirrus HD-OCT可以清晰地显示并测量大鼠视网膜各主要层次,且与组织学测量具有较好的相关性.视觉发育关键期内大鼠视网膜逐渐变薄,OCT测量主要表现为INL厚度和ONL至RPE层厚度减少.
배경인류화포유동물시각발육관건기내시망막처우발육계단,기각개아층적정학배렬이급세포적준학정위시시각신식정상전체적기출지일.빈역광학상관단층소묘(SD-OCT)가활체측량병련속관찰대서시망막후도적변화.목적료해시각발육관건기내SD대서시망막후도적변화규률.방법선택신생SD대서30지,기중10지우출생후14、18、21、24、42 d진행쌍안안저SD-OCT소묘,대시망막후도이급부분아층포괄내계막(ILM)지내총상층(IPL)、내핵층(INL)、외핵층(ONL)지시망막색소상피(RPE)층적후도진행생물측량.령취20지SD대서,분별우14、18、21、24、42 d각처사4지대서병적제쌍안,제작석사절편행소목정-이홍염색,대시망막후도이급부분아층결구진행측량.병대량충방법측량수거적상관성진행평개.결과대서시망막Cirrus HD-OCT도상유내향외가현시시망막IPL、INL、외총상층(OPL)、ONL、광감수기내절/외절(IS/OS)층이급RPE층등결구.비교각개시간점적시망막후도、ILM지IPL후도、INL후도이급ONL지RPE층후도,차이균유통계학의의(F=15.425,P=0.000;F=3.973,P=0.007;F=17.529,P=0.000;F=7.038,P=0.000).수대서일령적증가,시망막후도、INL후도급ONL지RPE층후도균정체감추세.측량시망막조직절편각대응층차후도,병여OCT측량결과진행선성회귀분석,결과표명량충방법측량적시망막후도、INL후도이급ONL지RPE층후도균정정상관(r=0.794,P=0.000;r=0.784,P=0.000;r=0.681,P=0.000).결론 Cirrus HD-OCT가이청석지현시병측량대서시망막각주요층차,차여조직학측량구유교호적상관성.시각발육관건기내대서시망막축점변박,OCT측량주요표현위INL후도화ONL지RPE층후도감소.
Background Retinal development continues during the early postnatal period in mammals.Correct arrangement of layers and precise location of various cells in the retina are vital for forming normal visual function during critical period plasticity.Spectral-domain optical coherence tomography(SD-OCT)provides highquality in vivo retinal imaging and the possibility to measure retinal thickness longitudinally. Objective The present study was to investigate the changes of retinal thickness during critical period plasticity in rats. Methods In vivo consecutive scanning of retinal image was performed in 10 SPF Sprague-Dawley rats at postnatal day 14(P14),P18,P21,P24 and P42 with SD-OCT,and retinal histopathological examination was used to detect retinal morphologic changes at the same postnatal ages in 20 matched rats.The whole retinal thickness,the thickness from inner limiting membrane(ILM)to inner plexiform layer(IPL),the thickness of inner nuclear layer(INL)and the thickness from outer nuclear layer(ONL)to retinal pigment epithelium(RPE)were measured using Cirrus HD-OCT system and HMIAS-2000 Imaging System in retinal sections.The measurement parameters by Cirrus HD-OCT and those by hematoxylin-eosin staining were compared.The use of animals followed the Statement of National Institute of Health (USA). Results In vivo high-resolution images of rat retinas with SD-OCT compared well with histology,which enabled quantitative comparison of the SD-OCT and histological data during critical period plasticity in rats.From P14 to P42,the retinal thickness gradually decreased with the increase of rat ages(F=15.425,P=0.000),and so were the thickness from ILM to IPL,the thickness of INL and the thickness from ONL to RPE(F=3.973,P=0.007;F=17.529,P=0.000;F=7.038,P=0.000).The retinal thickness,thickness of INL.thickness from ONL to RPE measured by Cirrus HD-OCT were significantly correlated with those measured by retinal sections among P14,P18,P21,P24 and P42 rats(r=0.794,P=0.000;r=0.784,P=0.000;r=0.681,P=0.000). Conclusion SD-OCT is a demonstratably valuable technology to study the structure of retinas in rats.The retinal thickness is shown to reduce in thickness throughout the development of the retina during critical period plasticity due to the decrease in thickness of INL and the distance from the ONL to RPE,as illustrated by OCT scanning.