CAJ | 학술논문

目的探讨艾滋病患者的白念珠菌唑类耐药株中唑类抗真菌药物(氟康唑、伊曲康唑、伏立康唑)的作用靶位基因ERG 11基因突变与耐药的关系.方法用PCR对临床分离的93株白念珠菌的Erg11基因进行扩增、测序,DNAman软件将测序结果与基因库中的X13296进行比对,将突变碱基翻译为氨基酸,确定是否发生错义突变.结果共检出40个碱基突变位点,包括27个同义突变位点和13个错义突变位点.耐一种药物的突变菌株中每株菌只发生一处错义突变或无错义突变,而耐二种或三种药物的菌株中每株菌还可以同时出现两处或三处错义突变.结论 ERG 11基因错义突变与白念珠菌耐药有关.
목적 탐토애자병환자적백념주균서류내약주중서류항진균약물(불강서、이곡강서、복립강서)적작용파위기인ERG 11기인돌변여내약적관계.방법 용PCR대림상분리적93주백념주균적Erg11기인진행확증、측서,DNAman연건장측서결과여기인고중적X13296진행비대,장돌변감기번역위안기산,학정시부발생착의돌변.결과 공검출40개감기돌변위점,포괄27개동의돌변위점화13개착의돌변위점.내일충약물적돌변균주중매주균지발생일처착의돌변혹무착의돌변,이내이충혹삼충약물적균주중매주균환가이동시출현량처혹삼처착의돌변.결론 ERG 11기인착의돌변여백념주균내약유관.
Objective To investigate the relationship between the mutation of ERG11 gene,a target of azole antifungal drugs (fluconazole,itraconazole,voriconazole),and azole-resistance in Candida albicans isolates from patients with AIDS.Methods Ninety-three Candida albicans strains were isolated from patients with AIDS.DNA was extracted from these isolates,and ERG11 gene was amplified by PCR followed by bidirectional sequencing.DNAman software was used to compare the resultant sequence with the reference sequence of ERG11 gene (GenBank accession no.X13296).Then,different base sequences were translated into amino acid sequences to determine whether missense mutations occured.Results A total of 40 mutation sites were identified in these isolates,including 27 silent mutations and 13 missense mutations.One or no missense mutation was detected in Candida albicans strains resistant to 1 antifungal agent,while those resistant to 2 or 3 antifungal agents simultaneously harbored 2 or 3 missense mutations.Conclusion The missense mutations in ERG11 gene are probably connected with azole resistance in Candida albicans.