中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
8期
618-620
,共3页
MIC%空气%工作场所%高效液相色谱法
MIC%空氣%工作場所%高效液相色譜法
MIC%공기%공작장소%고효액상색보법
MIC%Workplace air%High performance liquid chromatography
目的 建立工作场所空气中异氰酸甲酯(MIC)的高效液相色谱测定方法.方法 空气中蒸气态的MIC用浸渍过1-2-吡啶基哌嗪(1-2PP)的XAD-7管采集,MIC与荧光衍生剂1-2PP反应,在XAD-7管上生成稳定的荧光化合物,用乙腈解吸,经色谱柱分离,荧光检测器检测.结果 该法在0.01～10 μg/ml范围内呈线性关系,检出限为5.3×10-4 μg/ml;定量下限为0.01 μg/ml;最低检出浓度为3.3× 10-3 mg/m3(以采集3L空气样品计),相对标准偏差为2.2％～5.3％,平均解吸效率为90％,采样效率为100％,样品在冷藏状态下(5℃)能保存10d.结论 方法各项指标均符合GBT/T 210.4-2008《职业卫生标准制定指南第4部分:工作场所空气中化学物质测定方法》的要求,适用于工作场所空气中蒸气态异氰酸甲酯的检测.
目的 建立工作場所空氣中異氰痠甲酯(MIC)的高效液相色譜測定方法.方法 空氣中蒸氣態的MIC用浸漬過1-2-吡啶基哌嗪(1-2PP)的XAD-7管採集,MIC與熒光衍生劑1-2PP反應,在XAD-7管上生成穩定的熒光化閤物,用乙腈解吸,經色譜柱分離,熒光檢測器檢測.結果 該法在0.01～10 μg/ml範圍內呈線性關繫,檢齣限為5.3×10-4 μg/ml;定量下限為0.01 μg/ml;最低檢齣濃度為3.3× 10-3 mg/m3(以採集3L空氣樣品計),相對標準偏差為2.2％～5.3％,平均解吸效率為90％,採樣效率為100％,樣品在冷藏狀態下(5℃)能保存10d.結論 方法各項指標均符閤GBT/T 210.4-2008《職業衛生標準製定指南第4部分:工作場所空氣中化學物質測定方法》的要求,適用于工作場所空氣中蒸氣態異氰痠甲酯的檢測.
목적 건립공작장소공기중이청산갑지(MIC)적고효액상색보측정방법.방법 공기중증기태적MIC용침지과1-2-필정기고진(1-2PP)적XAD-7관채집,MIC여형광연생제1-2PP반응,재XAD-7관상생성은정적형광화합물,용을정해흡,경색보주분리,형광검측기검측.결과 해법재0.01～10 μg/ml범위내정선성관계,검출한위5.3×10-4 μg/ml;정량하한위0.01 μg/ml;최저검출농도위3.3× 10-3 mg/m3(이채집3L공기양품계),상대표준편차위2.2％～5.3％,평균해흡효솔위90％,채양효솔위100％,양품재랭장상태하(5℃)능보존10d.결론 방법각항지표균부합GBT/T 210.4-2008《직업위생표준제정지남제4부분:공작장소공기중화학물질측정방법》적요구,괄용우공작장소공기중증기태이청산갑지적검측.
Objective To establish the method of detecting the concentrations of methyl isocyanate (MIC) in air ofworkplaces with high performance liquid chromatographic (HPLC).Methods Samples are collected by XAD-7 tubes coated with 1-(2-pyridyl) piperazine (1-2PP).Samples are desorbed with acetonitrile (ACN) and analyzed by high performance liquid chromatography (HPLC) using a fluorescence detector.Results There was a linear relationship within the range of 0.01 ～10 μg/ml,and the detection limit was 5.3×10-4 μg/ml.The minimum detectable concentration was 0.01 μg/ml,and the lowest detected concentration was 3.3×10-3 mg/m3.The relative standard deviation was 2.2％～5.3％.The average desorption efficiency was 90％ and the sampling efficiencies were 100％.The samples could be stored for 10 days in cold storage condition.Conclusion The present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of MIC in workplace air.
