CAJ | 학술논문

目的了解引起医院内感染暴发临床分离的泛耐药肺炎克雷伯菌(Kpn)KPC型碳青霉烯酶基因blaKPC存在状况.方法 2008年11月至2009年7月从住院患者中分离19株泛耐药Kpn,采用改良Hodge试验检测菌株产碳青霉烯酶情况,采用PCR及序列分析的方法分析blaKPC基因.结果 19株泛耐药Kpn改良Hodge试验结果均阳性、blaKPC基因阳性率为100.0%,序列分析确认均为blaKPC-2亚型,其中HZ001号菌株(原始编号HZ9871)blaKPC-2基因序列已登录GenBank,注册号为GU086225.结论临床分离的泛耐药Kpn均携带blaKPC-2基因,而产KPC-2型碳青霉烯酶是分离菌株对碳青霉烯类抗生素耐药的主要原因.
목적 료해인기의원내감염폭발림상분리적범내약폐염극뢰백균(Kpn)KPC형탄청매희매기인blaKPC존재상황.방법 2008년11월지2009년7월종주원환자중분리19주범내약Kpn,채용개량Hodge시험검측균주산탄청매희매정황,채용PCR급서렬분석적방법분석blaKPC기인.결과 19주범내약Kpn개량Hodge시험결과균양성、blaKPC기인양성솔위100.0%,서렬분석학인균위blaKPC-2아형,기중HZ001호균주(원시편호HZ9871)blaKPC-2기인서렬이등록GenBank,주책호위GU086225.결론 림상분리적범내약Kpn균휴대blaKPC-2기인,이산KPC-2형탄청매희매시분리균주대탄청매희류항생소내약적주요원인.
Objective To investigate the status of genotype of the KPC(Klebsiella pneumoniae carbapenemase)-encoding genes in Pan-resistant K. Pneumoniae, isolated from the 98th Hospital of People' s Liberation Army, Huzhou district, Zhejiang province, China. Methods 19 strains of Pan-resistant K. Pneumoniae were isolated from the inpatients between November, 2008 and July,2009. Phenotypic confirmatory test for suspected carbapenemases production were carried out by Modified Hodge test. Carbapenemase gene of blaKPC was analyzed by PCR and verified by DNA sequencing. Results In 19 strains of K. Pneumoniae, the positive rates of Modified Hodge test and gene of blaKPC were both 100.0%. These genes all belonged to blaKPC-2 subtype confirmed by nucleotide sequence analysis. Among them, the blaKPC-2 gene sequence of the HZ001 strain (its original serial number was HZ9871 ) had been registered in GenBank (GenBank Accession Number: GU086225).Conclusion All of the Pan-resistant K. Pneumoniae isolated from the inpatients harbored blaKPC-2 type carbapenemases gene and causing an outbreak in a hospital. Carbapenemases that producing type KPC-2 might be the major reason which causing the resistance to Carbapenems antibiotics.