中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
6期
669-672
,共4页
杨建轩%闫惠平%檀玉芬%刘妍%马冬梅%李俊强%向代军%赵丹彤%周冬燕%孔祥沙
楊建軒%閆惠平%檀玉芬%劉妍%馬鼕梅%李俊彊%嚮代軍%趙丹彤%週鼕燕%孔祥沙
양건헌%염혜평%단옥분%류연%마동매%리준강%향대군%조단동%주동연%공상사
肝炎,自身免疫性%乙醇脱氢酶%酶联免疫吸附测定
肝炎,自身免疫性%乙醇脫氫酶%酶聯免疫吸附測定
간염,자신면역성%을순탈경매%매련면역흡부측정
Hepatitis,antoimmune%Alcohol dehydrogenase%Enzyme-linked imnmnosorbent assay
目的 建立血清抗乙醇脱氢酶(alcohol dehydrogenase,ADH)抗体ELISA法,并评价抗.ADH在诊断自身免疫性肝炎(AIH)中的价值.方法 用免疫印迹试验对酵母ADH与人血清抗.ADH之间的反应性进行验证.用酵母ADH建立检测血清抗-ADH的ELISA法.以67例AIH、94例原发性胆汁性肝硬化(PBC)、199例慢性乙型肝炎(CHB)、132例慢性丙型肝炎(CHC)、24例酒精性肝病(ALD)和99例结缔组织病(CTD)患者及31名健康对照者为研究对象,对其血清中的抗-ADH进行检测并统计其阳性率,阳性率的比较用χ2检验.结果 建立了一种检测人血清抗-ADH的ELISA法,并确定了最佳反应条件;免疫印迹试验证实酵母ADH与人血清抗-ADH有良好反应性.AIH患者血清抗-ADH阳性率为59.7%(40/67),高于健康对照组(0,χ2=31.271,P<0.05)、PBC组(6.4%,χ2=54.492,P<0.05)、CHB组(14.1%,χ2=54.848,P<0.05)、CHC组(21.2%,χ2=29.269,P<0.05)、ALD组(25.0%,χ2=8.512,P<0.05)和CTD组(43.4%,χ2=4.229.P<0.05).结论 AIH患者血清中抗-ADH阳性率高于其他肝病和某些自身免疫性疾病患者,可能对AIH有一定辅助诊断价值.
目的 建立血清抗乙醇脫氫酶(alcohol dehydrogenase,ADH)抗體ELISA法,併評價抗.ADH在診斷自身免疫性肝炎(AIH)中的價值.方法 用免疫印跡試驗對酵母ADH與人血清抗.ADH之間的反應性進行驗證.用酵母ADH建立檢測血清抗-ADH的ELISA法.以67例AIH、94例原髮性膽汁性肝硬化(PBC)、199例慢性乙型肝炎(CHB)、132例慢性丙型肝炎(CHC)、24例酒精性肝病(ALD)和99例結締組織病(CTD)患者及31名健康對照者為研究對象,對其血清中的抗-ADH進行檢測併統計其暘性率,暘性率的比較用χ2檢驗.結果 建立瞭一種檢測人血清抗-ADH的ELISA法,併確定瞭最佳反應條件;免疫印跡試驗證實酵母ADH與人血清抗-ADH有良好反應性.AIH患者血清抗-ADH暘性率為59.7%(40/67),高于健康對照組(0,χ2=31.271,P<0.05)、PBC組(6.4%,χ2=54.492,P<0.05)、CHB組(14.1%,χ2=54.848,P<0.05)、CHC組(21.2%,χ2=29.269,P<0.05)、ALD組(25.0%,χ2=8.512,P<0.05)和CTD組(43.4%,χ2=4.229.P<0.05).結論 AIH患者血清中抗-ADH暘性率高于其他肝病和某些自身免疫性疾病患者,可能對AIH有一定輔助診斷價值.
목적 건립혈청항을순탈경매(alcohol dehydrogenase,ADH)항체ELISA법,병평개항.ADH재진단자신면역성간염(AIH)중적개치.방법 용면역인적시험대효모ADH여인혈청항.ADH지간적반응성진행험증.용효모ADH건립검측혈청항-ADH적ELISA법.이67례AIH、94례원발성담즙성간경화(PBC)、199례만성을형간염(CHB)、132례만성병형간염(CHC)、24례주정성간병(ALD)화99례결체조직병(CTD)환자급31명건강대조자위연구대상,대기혈청중적항-ADH진행검측병통계기양성솔,양성솔적비교용χ2검험.결과 건립료일충검측인혈청항-ADH적ELISA법,병학정료최가반응조건;면역인적시험증실효모ADH여인혈청항-ADH유량호반응성.AIH환자혈청항-ADH양성솔위59.7%(40/67),고우건강대조조(0,χ2=31.271,P<0.05)、PBC조(6.4%,χ2=54.492,P<0.05)、CHB조(14.1%,χ2=54.848,P<0.05)、CHC조(21.2%,χ2=29.269,P<0.05)、ALD조(25.0%,χ2=8.512,P<0.05)화CTD조(43.4%,χ2=4.229.P<0.05).결론 AIH환자혈청중항-ADH양성솔고우기타간병화모사자신면역성질병환자,가능대AIH유일정보조진단개치.
Objective To establish the enzyme-linked immunosorbent assay (ELISA) for the detection of serum alcohol dehydrogenase (ADH) antibody and evaluate its role in its diagnosis of autoimmune hepatitis( AIH ). Methods The reactivity between yeast ADH and human anti-ADH serum antibody was tested by Western blot analysis. ELISA was established using yeast ADH. The method was applied in serums of 67 AIH patients,94 primary biliary cirrhosis(PBC) patients, 199 chronic hepatitis B (CHB) patients, 132 chronic hepatitis(CHC) patients, 24 alcohol hepatitis disease(ALD) patients, 99 connective tissue disease(CTD) patients and 31 healthy individuals. The positive rate of ADH antibody in the patients and healthy individuals was measured. The χ2 test was used to compare the positive rates. Results The ELISA method for detecting human anti-ADH serum antibody was established successfully and the optimum reaction conditions were defined. Western blot showed that yeast ADH has cross reactivity with human anti-ADH antibody. The positive rate of anti-ADH antibody in the AIH group [59. 7% ,40/67 ] was higher than that in the normal control group(0,χ2 = 31. 271 ,P <0. 05), PBC group (6. 4% ,χ2 =54. 492,P <0. 05), CHB group( 14. 1% ,χ2 =54. 848,P <0. 05) ,CHC group(21.2% ,χ2 = 29.269,P<0.05), ALl) group ( 25. 0% ,χ2 =8.512,P <0.05)and CTD group ( 43. 4% ,χ2 =4.229, P <0. 05). Conclusions Compared with the PBC, CHB, CHC, ALD and CTD group, the anti-ADH antibody positive rate in the serums of AIH was significantly increased. The antibody may be helpful to the diagnosis of autoimmune hepatitis.
