CAJ | 학술논문

白细胞介素-6(interleukin-6,IL-6)具有直接或间接的抗肿瘤活性，本组在以前的体内外实验中证明其具有明显的抑制肝癌作用。本文主要报告应用流式细胞仪和共聚焦显微镜检测IL-6对肝癌细胞(BEL-7402)凋亡的作用和该过程中Ca2＋转导机制。生长曲线描绘以及MTT分析结果表明，IL-6(6000u/ml)作用于BEL-7402细胞24小时后，生长抑制率达12%左右，而流式细胞仪结果显示IL-6(6000u/ml)作用于BEL-7402细胞24小时后，BEL-7402细胞凋亡率达8.2%。流式细胞仪分析还表明，IL-6(6000u/ml)作用于BEL-7402细胞24小时后，对照组平均FITC荧光值为1.03而IL-6(6000u/ml)组为0.759，也就是说，IL-6引起了bcl-2基因表达下降。激光共聚焦显微镜测定表明，IL-6(6000u/ml)作用于BEL-7402细胞后，胞浆[Ca2＋]c升高达2倍。若事先加入TG(thapsigargin)，15min后再加入IL-6，则抑制了胞浆内[Ca2＋]c升高；事先10min或5min分别加入EGTA和普鲁卡因(procaine)也有同样的抑制作用。上述结果表明，IL-6在一定剂量下可以诱导肝癌细胞BEL-7402发生细胞凋亡，该凋亡过程可能与Ca2＋转导及bcl-2基因表达下调有关。
백세포개소-6(interleukin-6,IL-6)구유직접혹간접적항종류활성，본조재이전적체내외실험중증명기구유명현적억제간암작용。본문주요보고응용류식세포의화공취초현미경검측IL-6대간암세포(BEL-7402)조망적작용화해과정중Ca2＋전도궤제。생장곡선묘회이급MTT분석결과표명，IL-6(6000u/ml)작용우BEL-7402세포24소시후，생장억제솔체12%좌우，이류식세포의결과현시IL-6(6000u/ml)작용우BEL-7402세포24소시후，BEL-7402세포조망솔체8.2%。류식세포의분석환표명，IL-6(6000u/ml)작용우BEL-7402세포24소시후，대조조평균FITC형광치위1.03이IL-6(6000u/ml)조위0.759，야취시설，IL-6인기료bcl-2기인표체하강。격광공취초현미경측정표명，IL-6(6000u/ml)작용우BEL-7402세포후，포장[Ca2＋]c승고체2배。약사선가입TG(thapsigargin)，15min후재가입IL-6，칙억제료포장내[Ca2＋]c승고；사선10min혹5min분별가입EGTA화보로잡인(procaine)야유동양적억제작용。상술결과표명，IL-6재일정제량하가이유도간암세포BEL-7402발생세포조망，해조망과정가능여Ca2＋전도급bcl-2기인표체하조유관。
Interleukin-6(IL-6) has shown direct or indirect antitumoractivity.In our previous experiments in vivo and vitro we hav e demonstrated that it produced pronounced antitumor effects on liver cancer .In this paper we mainly explored the apoptosis of BEL-7402 cells induced by IL-6 and the calcium signal transduction pathway during this process. The results of cell culture curve and MTT assay showed that IL-6(6000u/ml,for 24 hours) inhibited the growth of BEL-7402 cells by 12%,and th e results of Flow Cytometrical analysis demonstrate that IL-6(6000u/ml,for 24 h o urs)induced BEL-7402 cells to undergo apoptosis by 8.2%.The results of FCM thr ough BCL-2 staining suggested that the fluoresent value in control BEL-7402 cells wa s 1.03 while that in treated cells was 0.759 after the treatment of IL-6 for 24 hours.In other words,IL-6 caused the expression of bcl-2 to be down-regulated.Through confocal laser microscopy,we observed that after treatment of IL-6(6000 u /ml) cytosolic calcium [Ca2＋]c level determination in BEL-7402 inc rease d by 2-fold;Pretreated with TG for 15 minutes ,IL-6(6000u/ml)failed to induce the [Ca2＋]c increase;After treatment of EGTA for 10 minutes and procai ne for 5 minutes the [Ca2＋]c increase was also similarly inhibited. The above results demonstrated that IL-6 under certain dose can induce liver ca n cer cells BEL-7402 to undergo apoptosis and calcium signal transduction and dow n-regulation of the expression of bcl-2 may participate in this process