CAJ | 학술논문

为了更好地掌握代谢规律和代谢方式,代谢流作为一个关键而重要的变量需要测定.然而,由于代谢流测定的困难,在许多代谢研究中代谢流并没有被完全应用.在肌苷高产菌枯草芽孢杆菌中,利用得到的实验数据包括葡萄糖的消耗速率和代谢主产物以及副产物的形成速率,利用代谢通量平衡模型,得到糖酵解、三羟酸循环及磷酸戊糖途径的代谢流,并对其进行了分析.
위료경호지장악대사규률화대사방식,대사류작위일개관건이중요적변량수요측정.연이,유우대사류측정적곤난,재허다대사연구중대사류병몰유피완전응용.재기감고산균고초아포간균중,이용득도적실험수거포괄포도당적소모속솔화대사주산물이급부산물적형성속솔,이용대사통량평형모형,득도당효해、삼간산순배급린산무당도경적대사류,병대기진행료분석.
It is well recognized that metabolic fluxes are the key variables that must be determined in orderto understand metabolic regulation and patterns. However, owing to difficulties in measuring the flux values,evaluation of metabolic fluxes has not been an integral part of the most metabolic studies. Flux values formetabolites of glycolysis (EMP), tricarboxylic acid (TCA) cycle, and hexose monophosphate (HMP) pathwaywas obtained for batch cultures of inosine overproducing Bacilus subtilis by combining the information from thestoichiometry of key biosynthetic reactions with the experimental data on uptake rate of glucose and formationrates of metabolic product and byproducts.