实用口腔医学杂志
實用口腔醫學雜誌
실용구강의학잡지
JOURNAL OF PRACTICAL STOMATOLOGY
2009年
6期
816-819
,共4页
氟化物%成牙本质细胞%牙本质涎蛋白
氟化物%成牙本質細胞%牙本質涎蛋白
불화물%성아본질세포%아본질연단백
Fluoride%Odontoblasts%DSP
目的:研究短期高浓度氟对小鼠磨牙成牙本质细胞形态及牙本质涎蛋白(dentin sialoprotein, DSP)表达的影响,探讨氟对牙本质发育的作用机制.方法:选择4 d龄的ICR小鼠共32 只,随机分为2 组,每组16 只,各组中实验动物和对照动物各半.实验动物单次腹腔注射剂量分别为10 mg/kg体重和20 mg/kg体重的NaF,对照动物单次腹腔注射等剂量的NaCl,注射量均为10 μl/g, 24 h后处死动物.采用HE染色、免疫组化染色观察高浓度氟对小鼠磨牙不同分化阶段成牙本质细胞形态及DSP的表达,采用SPSS 13.0软件对数据进行分析.结果:实验组分泌期成牙本质细胞形态紊乱,正常的高柱状形态丧失,DSP的表达明显强于对照动物,差异有统计学意义(P<0.01),而成熟期成牙本质细胞未见明显变化.结论:短期高浓度氟能增强分泌期成牙本质细胞中DSP的表达,抑制成牙本质细胞的增殖分化及随后的基质合成与分泌,从而影响牙本质的发育.
目的:研究短期高濃度氟對小鼠磨牙成牙本質細胞形態及牙本質涎蛋白(dentin sialoprotein, DSP)錶達的影響,探討氟對牙本質髮育的作用機製.方法:選擇4 d齡的ICR小鼠共32 隻,隨機分為2 組,每組16 隻,各組中實驗動物和對照動物各半.實驗動物單次腹腔註射劑量分彆為10 mg/kg體重和20 mg/kg體重的NaF,對照動物單次腹腔註射等劑量的NaCl,註射量均為10 μl/g, 24 h後處死動物.採用HE染色、免疫組化染色觀察高濃度氟對小鼠磨牙不同分化階段成牙本質細胞形態及DSP的錶達,採用SPSS 13.0軟件對數據進行分析.結果:實驗組分泌期成牙本質細胞形態紊亂,正常的高柱狀形態喪失,DSP的錶達明顯彊于對照動物,差異有統計學意義(P<0.01),而成熟期成牙本質細胞未見明顯變化.結論:短期高濃度氟能增彊分泌期成牙本質細胞中DSP的錶達,抑製成牙本質細胞的增殖分化及隨後的基質閤成與分泌,從而影響牙本質的髮育.
목적:연구단기고농도불대소서마아성아본질세포형태급아본질연단백(dentin sialoprotein, DSP)표체적영향,탐토불대아본질발육적작용궤제.방법:선택4 d령적ICR소서공32 지,수궤분위2 조,매조16 지,각조중실험동물화대조동물각반.실험동물단차복강주사제량분별위10 mg/kg체중화20 mg/kg체중적NaF,대조동물단차복강주사등제량적NaCl,주사량균위10 μl/g, 24 h후처사동물.채용HE염색、면역조화염색관찰고농도불대소서마아불동분화계단성아본질세포형태급DSP적표체,채용SPSS 13.0연건대수거진행분석.결과:실험조분비기성아본질세포형태문란,정상적고주상형태상실,DSP적표체명현강우대조동물,차이유통계학의의(P<0.01),이성숙기성아본질세포미견명현변화.결론:단기고농도불능증강분비기성아본질세포중DSP적표체,억제성아본질세포적증식분화급수후적기질합성여분비,종이영향아본질적발육.
Objective: To study the effect of short exposure to high levels of fluoride on cellular structure and the DSP expression of odontoblasts of mice molars. Methods: 32 4-day-old ICR mice were randomly divided into two groups. The experimental animals were received a single intraperitoneal dose of 20 mg(n =8) andlO(n=8) mg NaF/kg( body weight) respectively. Equal doses of NaCl were given to the controls (n = 8 for each group). The injected volume was kept constant (10 μ/g). After 24 hours all mice were sacrificed. HE staining and immunohistochemical staining were used to observe the structural changes and the expression of DSP in odontoblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the results. Results: The secretory odontoblasts distorted and lost its normal column contour. The immunohistochemistry results demonstrated that the expression of DSP was more intense than that in control group. Results of statistics analysis showed there were significant differences between the two groups(P<0.01). No remarkable differences were found in mature odontoblasts. Conclusion: Short exposure to high concentration of fluoride can enhance the expression of DSP in the secretory odontoblasts,and inhibit differentiation of the odontoblasts and matrix secretion. This lead to the abnormal development of dentinogenesis.