CAJ | 학술논문

为探讨冬枣优良单株选择的遗传基础,本研究收集了河北、山东冬枣优良单株及对照冬枣共52株,以金丝小枣和尖枣做类外对照进行AFLP分子标记研究.实验选用16对M/E引物组合,共扩增出565条带,基于SM相似系数,利用UPGMA法对多态性谱带进行聚类分析,结果表明:冬枣优良单株群体内存在一定的遗传差异,但这种差异小于冬枣与金丝小枣和尖枣间的遗传距离,因此在聚类图中将冬枣、金丝小枣和尖枣分为3类;冬枣优良单株群体在聚类图中分成若干组,其中河北黄骅原始冬枣林古树和源于原始冬枣林的黄骅采穗圃冬枣优良单株,遗传距离相对较远;山东滨州各县的冬枣优良单株和沾化冬枣古树遗传距离较近,但存在个别变异较大的单株.上述结果证实冬枣是一个存在变异的群体、包含不同类型的品系,不同来源的冬枣优良单株存在一定的遗传变异,说明冬枣优良单株的优良性状不完全是立地条件和栽培技术引起的环境饰变,这一研究结果为冬枣的遗传改良和品种内选优提供了理论依据.
위탐토동조우량단주선택적유전기출,본연구수집료하북、산동동조우량단주급대조동조공52주,이금사소조화첨조주류외대조진행AFLP분자표기연구.실험선용16대M/E인물조합,공확증출565조대,기우SM상사계수,이용UPGMA법대다태성보대진행취류분석,결과표명:동조우량단주군체내존재일정적유전차이,단저충차이소우동조여금사소조화첨조간적유전거리,인차재취류도중장동조、금사소조화첨조분위3류;동조우량단주군체재취류도중분성약간조,기중하북황화원시동조림고수화원우원시동조림적황화채수포동조우량단주,유전거리상대교원;산동빈주각현적동조우량단주화첨화동조고수유전거리교근,단존재개별변이교대적단주.상술결과증실동조시일개존재변이적군체、포함불동류형적품계,불동래원적동조우량단주존재일정적유전변이,설명동조우량단주적우량성상불완전시입지조건화재배기술인기적배경식변,저일연구결과위동조적유전개량화품충내선우제공료이론의거.
This study analyzed genetic background of 52 advanced Dongzao( Zizyphus jujuba Mill.cv. Dongzao )trees and their control,Jinsixiaozao and Jianzao cultivars from Hebei and Shandong provinces using amplified fragment length polymorphism(AFLP)markers with 16 pair of Mse I/ EcoR I primer combinations.Cluster analysis was performed by using of unweighted pair group method with arithmatic mean(UPGMA)method based on SM similarity coefficient.The results were as follows: a total of 565 bands were amplified by 16 primer pairs,of which,225 were the polymorphic bands including Jinsixiaozao and Jianzao cultivars,99 were the polymorphic bands among Dongzao advanced trees.There was a little difference among the group of Dongzao advanced trees(the average similarity coefficient was 0.9792),though the difference was smaller than the difference between Dongzao with Jinsixiaozao and Jianzao cultivars(the similarity coefficient were 0.8294 and 0.7977,respectively).The three cultivars were divided into 3 groups and the group of Dongzao advanced trees were divided into several part in the cluster analyzing map.The genetic distance of the old Dongzao trees and the Dongzao advanced trees from Huanghua county,Hebei province were bigger than the ones from Binzhou city,Shandong province,the similarity coefficient were 0.9649 and 0.9946,respectively.But there were some individual candidate of aberrance among the latter.The above-mentioned results showed that there were some variation among Dongzao cultivar and the eximious traits of the advanced trees were not all attributed to the different growth condition and cultivating methods.So the results were very helpful to the genetic improvement and the selection of advanced trees of Dongzao cultivar.