CAJ | 학술논문

目的探讨Her2基因过表达的比例及其与临床病理之间的联系.方法收集乳腺浸润型导管癌标本50例,总结其临床及病理资料,应用石蜡包埋组织切片,以EnVision两步法进行免疫组织化学染色标记ER、PR及HER2,以金菩嘉DNA探针试剂盒行荧光原位杂交检测HER2基因.结果患者平均年龄55.5岁,病理组织分级Ⅰ级11例,Ⅱ级30例,Ⅲ级9例.术后分期Ⅰ期13例,ⅡA期15例,ⅡB期13例,ⅢA期6例,ⅢB期2例,ⅢC期1例.淋巴结中位转移率6.91%.ER阳性33例,PR阳性32例,IHC法HER2阳性40例,FISH阳性33例,阴性17例.FISH法检测HER2基因与HER2蛋白过表达之间相关(P<0.05),其与病理分级、术后分期、淋巴结转移率及ER、PR的表达无相关(P>0.05),3例脉管内瘤栓及3例原发瘤数大于1者,FISH均为阳性.结论 FISH检测HER2基因扩增和免疫组化检测HER2蛋白一致性较好,HER2基因与脉管内瘤栓及多个原发瘤有一定关系,但其作为间接预测预后的指标有待于进一步研究.
목적 탐토Her2기인과표체적비례급기여림상병리지간적련계.방법 수집유선침윤형도관암표본50례,총결기림상급병리자료,응용석사포매조직절편,이EnVision량보법진행면역조직화학염색표기ER、PR급HER2,이금보가DNA탐침시제합행형광원위잡교검측HER2기인.결과 환자평균년령55.5세,병리조직분급Ⅰ급11례,Ⅱ급30례,Ⅲ급9례.술후분기Ⅰ기13례,ⅡA기15례,ⅡB기13례,ⅢA기6례,ⅢB기2례,ⅢC기1례.림파결중위전이솔6.91%.ER양성33례,PR양성32례,IHC법HER2양성40례,FISH양성33례,음성17례.FISH법검측HER2기인여HER2단백과표체지간상관(P<0.05),기여병리분급、술후분기、림파결전이솔급ER、PR적표체무상관(P>0.05),3례맥관내류전급3례원발류수대우1자,FISH균위양성.결론 FISH검측HER2기인확증화면역조화검측HER2단백일치성교호,HER2기인여맥관내류전급다개원발류유일정관계,단기작위간접예측예후적지표유대우진일보연구.
Purpose To detect the HER2 gene amplification by fluorescence in-situ hybridization(FISH) and to explore its clinicopathologic relationship with the breast cancer.Methods A prospective study was conducted in 50 cases of breast invasive ductal carcinoma from Beijing Toren Hospital. Clinicopathologic data were summarized and FISH was performed in the paraffin-embedded sections for HER2 gene amplificayion using DNA probe, and immunohistochemical stain for ER, PR and HER2.Results The average age of the patients was 55.5 years. The pathologic grading showed that 11 cases were in grade Ⅰ, 30 cases in grade Ⅱ, and 9 cases in grade Ⅲ. The TNM staging showed that 13 cases were in stage Ⅰ,15 cases in ⅡA,13 cases in ⅡB,6 cases in ⅢA,2 cases in ⅢB,and 1 case in ⅢC.The median metastasis rate of lymph node was 6.91%.33 cases were positive for ER,and 32 cases positive for PR.For HER2 detection, 40 cases were positive by IHC and 33 positive by FISH. HER2 gene amplification by FISH was closely related with the expression of HER2 protein by immunohistochemistry, but not significantly related with pathologic grading, The TNM staging, median lymph node metastasis rate, ER and PR status (P>0.05). FISH test was positive in 3 cases of tumor embolus and 3 cases of multiple primary tumors. Conclusions FISH and immunohistochemistry for detecting HER2 have a good conformance, HER2 gene amplification may be related with tumor embolus and multiple primary tumors, but it can not be used as an indirect marker to predict the prognosis.