CAJ | 학술논문

背景:血管细胞外基质作为来源于血管组织的天然生物支架材料,在植入宿主体内后能迅速刺激血管生成,加速组织工程化组织血管化,但其确切机制还不清楚.目的:探讨血管细胞外基质在修复输尿管缺损中释放血管内皮生长因子增强血管化的作用.设计、时间及地点:细胞学体外观察,于2009-04/08在武汉大学第一临床医学院生物医学工程实验室完成.材料:取5只供体兔的腹主动脉经手术取出制备血管细胞外基质.方法:用ELISA法体外检测血管细胞外基质在PBS孵育液中血管内皮生长因子的含量,应用MTT法体外检测血管细胞外基质对内皮细胞的增殖作用;应用家兔同种异体血管细胞外基质体内修复输尿管缺损,术后不同时间检测兔输尿管缺损修复情况及血管再生情况.主要观察指标:血管细胞外基质内血管内皮细胞生长因子含量的检测,血管细胞外基质对内皮细胞的增殖作用及对输尿管缺损修复的影响.结果:体外实验显示血管细胞外基质在PBS孵育液中血管内皮生长因子含量峰值为(124.10±1.42)ng/L,对内皮细胞有促进增殖作用.体内移植修复试验显示术后2周移植物可见新生毛细血管,腔内面边缘部见移行上皮细胞爬行,管壁有平滑肌细胞长入;第8周移植物内大量毛细血管生成,且管腔增大,移行上皮细胞层增厚,平滑肌规则排列成束;术后16周血管细胞外基质修复区已接近正常输尿管组织结构.结论:血管细胞外基质作为生物支架材料,在降解的同时能释放血管内皮生长因子刺激血管再生,有可能成为一种理想的输尿管重建材料.
배경:혈관세포외기질작위래원우혈관조직적천연생물지가재료,재식입숙주체내후능신속자격혈관생성,가속조직공정화조직혈관화,단기학절궤제환불청초.목적:탐토혈관세포외기질재수복수뇨관결손중석방혈관내피생장인자증강혈관화적작용.설계、시간급지점:세포학체외관찰,우2009-04/08재무한대학제일림상의학원생물의학공정실험실완성.재료:취5지공체토적복주동맥경수술취출제비혈관세포외기질.방법:용ELISA법체외검측혈관세포외기질재PBS부육액중혈관내피생장인자적함량,응용MTT법체외검측혈관세포외기질대내피세포적증식작용;응용가토동충이체혈관세포외기질체내수복수뇨관결손,술후불동시간검측토수뇨관결손수복정황급혈관재생정황.주요관찰지표:혈관세포외기질내혈관내피세포생장인자함량적검측,혈관세포외기질대내피세포적증식작용급대수뇨관결손수복적영향.결과:체외실험현시혈관세포외기질재PBS부육액중혈관내피생장인자함량봉치위(124.10±1.42)ng/L,대내피세포유촉진증식작용.체내이식수복시험현시술후2주이식물가견신생모세혈관,강내면변연부견이행상피세포파행,관벽유평활기세포장입;제8주이식물내대량모세혈관생성,차관강증대,이행상피세포층증후,평활기규칙배렬성속;술후16주혈관세포외기질수복구이접근정상수뇨관조직결구.결론:혈관세포외기질작위생물지가재료,재강해적동시능석방혈관내피생장인자자격혈관재생,유가능성위일충이상적수뇨관중건재료.
BACKGROUND: Vessel extracellular matrix (VECM) is a natural scaffold material obtained from vascular tissues, which can stimulate angiogenesis and accelerate vascularization of tissue-engineered graft, however, the mechanism is poorly understood.OBJECTIVE: To explore the vascularization effects of release of vascular endothelial growth factor (VEGF) from VECM in ureteral reconstitution.DESIGN, TIME AND SETTING: An in vitro cytology observation. The experiment was performed at the Biomedical Engineering Laboratory of First Clinical Medical Science College, Wuhan University, between April and August in 2009.MATERIALS: Abdominal aorta was obtained from 5 rabbits to prepare VECM.METHODS: The VEGF released from VECM in vitro was evaluated by ELISA, the effects of cell proliferation by the released VEGF was detected by MTT colorimetric assay. The defected ureters of rabbits were repaired by homologous VECM in vivo.Then the recovery of the defected ureters and the situation of vasculogenesis were detected at different time point.MAIN OUTCOME MEASURES: The detection of VEGF contents in VECM; and the effects of VECM on vascular endothelial cell proliferation and ureteral reconstitution.RESULTS: In vitro experiment presented that the peak amplitude concentration of VEGF released from VECM in PBS solution was (124.10±1.42) ng/L, which showed proliferative effect on vascular endothelial cells. In vivo, there were some blood vessels on the VECM at 2 weeks after implantation. Epithelial coverage was evident in the lumen of the marginal part of the VECM grafts and the smooth muscle extended from the transition zone. After 8 weeks, the quantity of the blood vessel was increased and the caliber of the blood vessels became wide. There was thickness epithelial lamina in the graft, and the muscle fibers had an organized spatial alignment, forming variably sized bundles. After 16 weeks, there were no significant differences between the regenerative tissue and the normal tissue in morphology.CONCLUSION: The homologous VECM can release VEGF when implanted as tissue engineer biomaterial and might be an ideal replacement biomaterial for ureteral reconstitution.