分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2010年
3期
44-46
,共3页
单扫描极谱法%1-(2-吡啶偶氮)-2-萘酚(PAN)%牛血清白蛋白(BSA)%人血清白蛋白(HSA)络合物
單掃描極譜法%1-(2-吡啶偶氮)-2-萘酚(PAN)%牛血清白蛋白(BSA)%人血清白蛋白(HSA)絡閤物
단소묘겁보법%1-(2-필정우담)-2-내분(PAN)%우혈청백단백(BSA)%인혈청백단백(HSA)락합물
Single sweep polarography%1-(2-Pyridylazo)-2-naphthol (PAN)%Bovine serum albumin (BSA)%Human serum albumin (HSA)%Complex
在0.05 mol/L HAc 溶液中,PAN-牛血清白蛋白/人血清白蛋白络合物在 -0.58 V (vs. SCE)处产生一灵敏吸附还原峰,峰电位较之游离PAN还原峰负约0.28 V,峰电流与牛血清白蛋白、人血清白蛋白浓度在0.1～12 mg/L,0.1～11 mg/L范围内呈线性关系;检测限均为0.05 mg/L. 运用该法测定了人血清样品蛋白质含量.
在0.05 mol/L HAc 溶液中,PAN-牛血清白蛋白/人血清白蛋白絡閤物在 -0.58 V (vs. SCE)處產生一靈敏吸附還原峰,峰電位較之遊離PAN還原峰負約0.28 V,峰電流與牛血清白蛋白、人血清白蛋白濃度在0.1～12 mg/L,0.1～11 mg/L範圍內呈線性關繫;檢測限均為0.05 mg/L. 運用該法測定瞭人血清樣品蛋白質含量.
재0.05 mol/L HAc 용액중,PAN-우혈청백단백/인혈청백단백락합물재 -0.58 V (vs. SCE)처산생일령민흡부환원봉,봉전위교지유리PAN환원봉부약0.28 V,봉전류여우혈청백단백、인혈청백단백농도재0.1～12 mg/L,0.1～11 mg/L범위내정선성관계;검측한균위0.05 mg/L. 운용해법측정료인혈청양품단백질함량.
A sensitive reduction wave of PAN-proteins at -0.58 V (vs. SCE)has been observed by using single sweep polarography in 0.05 mol/L acetic acid and 7.5×10~(-6)mol/L PAN solution. The peak potential is 0.28 V more negative than that of PAN. The second order derivative peak height is linearly proportional to the concentration of BSA from 0.1 to 12 mg/L and to the concentration of HSA from 0.1 to 11 mg/L. The detection limit is 0.05 mg/L both for BSA and for HSA. The method has been used for the determination of total protein in human serum samples. The result is satisfactory.
