CAJ | 학술논문

采用解剖镜观察及组织切片和电镜技术,研究了三疣梭子蟹促雄腺的位置、形态、显微及亚显微结构.结果发现,三疣梭子蟹促雄腺1对,附着在左右射精管的表面,呈不规则索状.随着个体繁殖周期的变化,促雄腺结构各异,可划分为增殖期、合成期和分泌期3个发育时期.腺体进行全浆分泌,有A、B 2种细胞类型,A型细胞具常染色质,粗面内质网和线粒体丰富,B型细胞具异染色质,细胞核固缩.处于增殖期的促雄腺量少,体积小,细胞界限清晰;合成期促雄腺体积大,呈索状,内质网和线粒体发达,A型细胞居多;分泌期分为分泌前期和分泌后期,分泌前期促雄腺体积较大,以B型细胞居多,分泌后期促雄腺体积小,部分有脱落现象,细胞器解体,细胞内存在大量空泡,有的细胞已经解体.
채용해부경관찰급조직절편화전경기술,연구료삼우사자해촉웅선적위치、형태、현미급아현미결구.결과발현,삼우사자해촉웅선1대,부착재좌우사정관적표면,정불규칙색상.수착개체번식주기적변화,촉웅선결구각이,가화분위증식기、합성기화분비기3개발육시기.선체진행전장분비,유A、B 2충세포류형,A형세포구상염색질,조면내질망화선립체봉부,B형세포구이염색질,세포핵고축.처우증식기적촉웅선량소,체적소,세포계한청석;합성기촉웅선체적대,정색상,내질망화선립체발체,A형세포거다;분비기분위분비전기화분비후기,분비전기촉웅선체적교대,이B형세포거다,분비후기촉웅선체적소,부분유탈락현상,세포기해체,세포내존재대량공포,유적세포이경해체.
The location,morphology,microstructure and ultrastructure of the androgenic gland in swimming crab Portunus trituberculatus were studied under zoom stereo microscope,histological section and electron microscope. The results showed that a pair of androgenic glands was found on the surface of the ejaculatory duct. The cytological development of the irregular cord-shape gland can be distinctly divided into three stages:proliferation phase,synthesis phase and secretion phase. The gland secreted in a holocrine fashion,and had two types of cells:type A,whose nuclei were rich in euchromatin,mitochondria and rough endoplasmic reticulum,and type B,which had nuclei rich in heterochromation,and showed karyopyknosis. In proliferation phase the androgenic glands were short and the glandular cells were of small amount,and had a distinct cell boundary. In synthesis phase,the androgenic glands were bigger in size than that in the proliferation phase. The glands with more type A cells appeared like a cord-shape,and had well-developed endoplasmic reticulum and mitochondria. In the prophase of the secretion phase,the androgenic glands were still big with more type B cells,and in the anaphase of the secretion phase,the androgenic glands were smaller in size,and some were broken down. There were many vacuoles in the glandular cells.