CAJ | 학술논문

[目的]为了明确茉莉酸对小麦白粉病抗性的诱导作用和对抗病相关基因的激活作用,以及抗病性变化与基因表达变化之间的相关性,探索小麦抗白粉病分子机理.[方法]以田间表现不同的代表性感白粉病小麦品种"中国春"、"濮麦9号"和"周麦18"为材料,用茉莉酸甲酯(methyl jasmonate,MeJA)处理小麦幼苗叶片进行诱导,通过离体叶段培养法接种白粉菌(Blumeria graminis f.sp.tritici,Bgt)进行抗性鉴定;用实时定量PCR技术检测叶片中PR1(PR1.1)、PR2(β,1-3葡聚糖苷酶)、PR3(几丁质酶)、PR4、PR5(类甜蛋白)、PR9(TaPERO,过氧化物酶)、PR10、TaGLP2a(类胚素蛋白)和Ta-JA2(茉莉酸甲酯诱导蛋白)基因的表达变化.[结果]MeJA处理可以显著提高"中国春"、"濮麦9号"和"周麦18"对白粉菌的抗性水平.诱导抗性可以从MeJA处理后12～96h检测到,24 h达到峰值.虽然存在一定差异,但MeJA对3个品种中除TaGLP2a外的8个抗病相关基因的表达有显著激活作用,在处理12、24或48h 后达到峰值.MeJA对PR9和PR1的诱导作用最强,可达100倍,对PR2、PR4、PR5、PR3、PR10和Ta-JA2的诱导作用强,可达10-70倍;对TaGLP2a没有明显作用.茉莉酸诱导的抗病性提高与8个抗病相关基因的表达增强呈正相关.[结论]茉莉酸诱导的抗病性增强与抗病相关基因的表达增强呈正相关,茉莉酸信号传导途径在小麦抗白粉病反应中起作用,对这一途径的调控可提高小麦的白粉病抗性.
[목적]위료명학말리산대소맥백분병항성적유도작용화대항병상관기인적격활작용,이급항병성변화여기인표체변화지간적상관성,탐색소맥항백분병분자궤리.[방법]이전간표현불동적대표성감백분병소맥품충"중국춘"、"복맥9호"화"주맥18"위재료,용말리산갑지(methyl jasmonate,MeJA)처리소맥유묘협편진행유도,통과리체협단배양법접충백분균(Blumeria graminis f.sp.tritici,Bgt)진행항성감정;용실시정량PCR기술검측협편중PR1(PR1.1)、PR2(β,1-3포취당감매)、PR3(궤정질매)、PR4、PR5(류첨단백)、PR9(TaPERO,과양화물매)、PR10、TaGLP2a(류배소단백)화Ta-JA2(말리산갑지유도단백)기인적표체변화.[결과]MeJA처리가이현저제고"중국춘"、"복맥9호"화"주맥18"대백분균적항성수평.유도항성가이종MeJA처리후12～96h검측도,24 h체도봉치.수연존재일정차이,단MeJA대3개품충중제TaGLP2a외적8개항병상관기인적표체유현저격활작용,재처리12、24혹48h 후체도봉치.MeJA대PR9화PR1적유도작용최강,가체100배,대PR2、PR4、PR5、PR3、PR10화Ta-JA2적유도작용강,가체10-70배;대TaGLP2a몰유명현작용.말리산유도적항병성제고여8개항병상관기인적표체증강정정상관.[결론]말리산유도적항병성증강여항병상관기인적표체증강정정상관,말리산신호전도도경재소맥항백분병반응중기작용,대저일도경적조공가제고소맥적백분병항성.
[Objective]This study was carried out to determine the induction effect of jasmonic acid (JA) on powdery mildew resistance in wheat, the activation effect on the expressions of plant disease resistance related genes, and to investigate the relationship between the induced resistance and the gene expression patterns.[Method]Three powdery mildew susceptible cultivars of "Chinese Spring" , "Pumai 9".and "Zhoumai 18" typically representing different phenotypes in the field were employed. The powdery mildew was assessed by detached leaf assay, and real time quantitative RT-PCR was used to determine the expression patterns of 9 disease resistance related genes of PR1 (PR1.1), PR2(β, 1-3 glucanase), PR3(chitinase), PR4(Wheatwin1), PR5(thaumatin-like protein), PR9(TaPERO, peroxidase), PR10, TaGLP2a resistances of "Chinese Spring" , "Pumai 9" and "Zhoumai 18".The induced powdery mildew resistance could be detected from 12 h to 96 h after MeJA treatment, and the peak value Was at 24 h.Though there were differences between the three cultivars, MeJA significantly effect on the expressions of the 8 disease resistance related genes except TaGLP2a, and the peak values were at 12h, 24 h or 48 h after treatments. The strongest activation of MeJA was on PR9 and PR1 that their expressions could reach more than 100 times of the untreated samples. MeJA strongly activated PR2、PR4、PR5、PR3、PR10 and tA-JA2, their expression could reach 10 to 70 times, and there was almost no activation effect on TaGLP2a.The induced powdery mildew resistance positively correlated with the induced expressions of the 8 disease related genes.[Conclusion]The induced powdery mildew resistance positively correlated with the induced expressions of the disease related genes. Jasmonate signalling plays a role in defence against Blumeria graminis f.sp.tritici.and future manipulation of this pathway may improve powdery mildew resistance in wheat.