中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
2期
104-107
,共4页
聂静敏%蔡卫平%胡凤玉%李凌华%刘金峰%许敏%关玉娟%唐小平
聶靜敏%蔡衛平%鬍鳳玉%李凌華%劉金峰%許敏%關玉娟%唐小平
섭정민%채위평%호봉옥%리릉화%류금봉%허민%관옥연%당소평
肝炎,丙型,慢性%核苷脱氨酶类%单核细胞%病毒载量%RNA信使%RNA,病毒
肝炎,丙型,慢性%覈苷脫氨酶類%單覈細胞%病毒載量%RNA信使%RNA,病毒
간염,병형,만성%핵감탈안매류%단핵세포%병독재량%RNA신사%RNA,병독
Hepatitis C,chronic%Nucleoside deaminases%Monocytes%Viral load%RNA messenger%RNA,viral
目的 探讨慢性丙型肝炎患者外周血单个核细胞(PBMC)APOBEC3G mRNA表达水平与HCV慢性感染的关系.方法 用TaqMan实时(RT)-PCR法检测49例慢性丙型肝炎患者以及31名健康人PBMC中APOBEC3G mRNA的水平,同时检测丙型肝炎患者外周血HCV RNA载量.所得数据采用SPSS 11.0分析软件进行t检验和回归分析.结果 慢性丙型肝炎患者PBMC中APOBEC3G mRNA的表达水平为(1.5±1.9)×10-5拷贝/mL,低于健康对照组(5.2±5.5)×10-5拷贝/mL,两组比较差异具有统计学意义(t=-3.005,P＜0.01),而与外周血HCVRNA载量无明显相关性(r=-0.082,P＞0.05).结论 HCV感染抑制APOBEC3G的表达,但APOBEC3G不影响HCV复制.
目的 探討慢性丙型肝炎患者外週血單箇覈細胞(PBMC)APOBEC3G mRNA錶達水平與HCV慢性感染的關繫.方法 用TaqMan實時(RT)-PCR法檢測49例慢性丙型肝炎患者以及31名健康人PBMC中APOBEC3G mRNA的水平,同時檢測丙型肝炎患者外週血HCV RNA載量.所得數據採用SPSS 11.0分析軟件進行t檢驗和迴歸分析.結果 慢性丙型肝炎患者PBMC中APOBEC3G mRNA的錶達水平為(1.5±1.9)×10-5拷貝/mL,低于健康對照組(5.2±5.5)×10-5拷貝/mL,兩組比較差異具有統計學意義(t=-3.005,P＜0.01),而與外週血HCVRNA載量無明顯相關性(r=-0.082,P＞0.05).結論 HCV感染抑製APOBEC3G的錶達,但APOBEC3G不影響HCV複製.
목적 탐토만성병형간염환자외주혈단개핵세포(PBMC)APOBEC3G mRNA표체수평여HCV만성감염적관계.방법 용TaqMan실시(RT)-PCR법검측49례만성병형간염환자이급31명건강인PBMC중APOBEC3G mRNA적수평,동시검측병형간염환자외주혈HCV RNA재량.소득수거채용SPSS 11.0분석연건진행t검험화회귀분석.결과 만성병형간염환자PBMC중APOBEC3G mRNA적표체수평위(1.5±1.9)×10-5고패/mL,저우건강대조조(5.2±5.5)×10-5고패/mL,량조비교차이구유통계학의의(t=-3.005,P＜0.01),이여외주혈HCVRNA재량무명현상관성(r=-0.082,P＞0.05).결론 HCV감염억제APOBEC3G적표체,단APOBEC3G불영향HCV복제.
Objective To study the relationship between APOBEC3G mRNA level in peripheral blood mononuclear cells (PBMC) and serum hepatitis C viral RNA level in patients with chronic hepatitis C infection. Methods TaqMan real-time fluorescence relative quantitative polymerase chain reaction (RT-PCR) was used to quantify APOBEC3G mRNA levels in PBMC from 49 patients with chronic hepatitis C (CHC) and 31 healthy subjects. The relationship between APOBEC3G mRNA level and hepatitis C virus (HCV) viral load was analyzed. SPSS11. 0 statistics software was used for t test and regression analysis. Results APOBEC3G mRNA level in CHC patients [(1.5×10-5±1.9×10-5 ) copy/mL] was significantly lower than that [( 5. 2 × 10-5 ± 5. 5 × 10-5 ) copy/mL] in the healthy control subjects (t=-3.005, P＜0.01). While APOBEC3G mRNA level was not related with HCV viral loads (r=-0.082, P＞0.05). Conclusion HCV has an inhibitive effect on APOBEC3G expression, whereas APOBEC3G doesn't affect HCV replication directly in vivo.
