中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2008年
5期
376-379
,共4页
李大卫%彭志海%黄伟翼%杨兆瑞%吴晴
李大衛%彭誌海%黃偉翼%楊兆瑞%吳晴
리대위%팽지해%황위익%양조서%오청
胃肿瘤%多药耐药蛋白质类%氨基末端激酶
胃腫瘤%多藥耐藥蛋白質類%氨基末耑激酶
위종류%다약내약단백질류%안기말단격매
Stomach neoplasms%Muhidrng resistance-associated proteins%N-terminal kinase
目的 研究c-jun氨基末端激酶(e-jun N-terminal kinase,JNK)的磷酸化活性形式p-JNK与多药耐药蛋白P糖蛋白(p-glycoprotein,P-gP)、多药耐药相关蛋白1(multidrng resistance associated proteinl,MRP1)和肺耐药相关蛋白(lung resistance protein,LRP)在胃癌组织中的表达及p-JNK在胃癌耐药中的作用.方法 通过免疫组化方法研究168例胃癌和27例正常胃组织的组织芯片中p-JNK和P-gP、MRP1、LRP的表达及其关系.结果 p-JNK和耐药蛋白P-gP、MRP1、LRP在胃癌组织中的阳性表达率分别为45.8%、51.8%、45.8%和55.4%,均明显高于在正常胃组织中的表达.p-JNK与肿瘤大小、浸润深度、脉管浸润、淋巴结转移和远处转移显著相关.p-JNK与P-gP和MRPl表达正相关(P＜0.01).Kaplan-Meier分析显示p-JNK和P-gp、MRP1阳性组的预后较阴性组差(P＜0.01).结论 p-JNK高表达参与胃癌的恶性生物学行为,通过上调耐药蛋白P-gp和MRP1的表达参与胃癌化疗耐药的形成;p-JNK可成为评价胃癌预后和耐药的有价值指标.
目的 研究c-jun氨基末耑激酶(e-jun N-terminal kinase,JNK)的燐痠化活性形式p-JNK與多藥耐藥蛋白P糖蛋白(p-glycoprotein,P-gP)、多藥耐藥相關蛋白1(multidrng resistance associated proteinl,MRP1)和肺耐藥相關蛋白(lung resistance protein,LRP)在胃癌組織中的錶達及p-JNK在胃癌耐藥中的作用.方法 通過免疫組化方法研究168例胃癌和27例正常胃組織的組織芯片中p-JNK和P-gP、MRP1、LRP的錶達及其關繫.結果 p-JNK和耐藥蛋白P-gP、MRP1、LRP在胃癌組織中的暘性錶達率分彆為45.8%、51.8%、45.8%和55.4%,均明顯高于在正常胃組織中的錶達.p-JNK與腫瘤大小、浸潤深度、脈管浸潤、淋巴結轉移和遠處轉移顯著相關.p-JNK與P-gP和MRPl錶達正相關(P＜0.01).Kaplan-Meier分析顯示p-JNK和P-gp、MRP1暘性組的預後較陰性組差(P＜0.01).結論 p-JNK高錶達參與胃癌的噁性生物學行為,通過上調耐藥蛋白P-gp和MRP1的錶達參與胃癌化療耐藥的形成;p-JNK可成為評價胃癌預後和耐藥的有價值指標.
목적 연구c-jun안기말단격매(e-jun N-terminal kinase,JNK)적린산화활성형식p-JNK여다약내약단백P당단백(p-glycoprotein,P-gP)、다약내약상관단백1(multidrng resistance associated proteinl,MRP1)화폐내약상관단백(lung resistance protein,LRP)재위암조직중적표체급p-JNK재위암내약중적작용.방법 통과면역조화방법연구168례위암화27례정상위조직적조직심편중p-JNK화P-gP、MRP1、LRP적표체급기관계.결과 p-JNK화내약단백P-gP、MRP1、LRP재위암조직중적양성표체솔분별위45.8%、51.8%、45.8%화55.4%,균명현고우재정상위조직중적표체.p-JNK여종류대소、침윤심도、맥관침윤、림파결전이화원처전이현저상관.p-JNK여P-gP화MRPl표체정상관(P＜0.01).Kaplan-Meier분석현시p-JNK화P-gp、MRP1양성조적예후교음성조차(P＜0.01).결론 p-JNK고표체삼여위암적악성생물학행위,통과상조내약단백P-gp화MRP1적표체삼여위암화료내약적형성;p-JNK가성위평개위암예후화내약적유개치지표.
Objective To investigate the clinicopathogenical significance of phosphorylation type of c-jun N-terminal kinase(p-JNK)and muhidmg resistance protein P-glycopretein(P-gP),multidrug resistance proteinl(MRP1)and lung resistance protein(LRP)in gastric cancer. Methods The expression of p-JNK,P-gP,MRP1 and LRP was detected in a tissue microarray containing 168 spots of gastric cancer tissue and 27 spots of normal gastric tissue by immunohistochemistry.Results The positive expression rate of p-JNK,P-gp,MRP1 and LRP in gastric cancer wag 45.8%,51.8%,45.8%and 55.4%respectively,which Was significantly higher than that in normal gastric tissue(P＜0.05).The p-JNK expression correlated with depth of invasion(P＜0.01),histology grade(P＜0.05),vessel invasion (P＜0.01),lymph node metastasis and distant metastasis(P＜0.01).The expression of p-JNK,P-gp and MRP1 in a positive relationship(P＜0.05).Kaplan-Meier analysis revealed a significant impact on survival by p-JNK,P-gp and MRPl(P＜0.05). Conclusion The p-JNK expression in gastric cancer is correlated with malignant biological behavior and may be involved in the chemotherapeutic resistance by upregulating the expression of P-g[ and MRP1.
